TaqMan™ Rodent GAPDH Control Reagents - FAQs

View additional product information for TaqMan™ Rodent GAPDH Control Reagents - FAQs (4308313)

6 product FAQs found

What type of control RNA is included in the TaqMan Rodent GAPDH kit (P/N 4308313)?

The RNA included in the TaqMan Rodent GAPDH kit is total RNA extracted from P388D1 lymphoma mouse cell line.

What is the GenBank accession number of the sequence that was used to design the primers and probe for the TaqMan Rodent GAPDH Control Reagent Kit (P/N 4308313)?

The GenBank accession number used is NM_008084. For more information on this kit, please refer to the TaqMan Rodent GAPDH kit protocol.

Since the TaqMan Rodent GAPDH Control Reagents (Cat. No. 4308313) will also amplify pseudogenes and/or genomic DNA, is there some equation that can be used to correct for genomic DNA contamination?

There is no equation for the correction of data for genomic contamination; however, a rule of thumb is that if the Ct difference between your target and the contaminant is 10 or more, you can assume it is not having an effect in your reaction. Please consult the TaqMan Rodent GAPDH Control Reagents Protocol for more information on these reagents. Enter the catalog number in the main Search field on our web site to view the product detail page and the Protocol.

Do the human and rodent GAPDH primers and TaqMan probe sequences manufactured by Applied Biosystems detect genomic DNA?

This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:

- TaqMan GAPDH Control Reagents, human, 402869, JOE: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH for human, 4326317E, VIC : Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4310884E, VIC: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764F, FAM: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764T, FAM: Does not detect genomic DNA
- TaqMan Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: Does not detect genomic DNA

In the TaqMan Rodent GAPDH Control Reagents kit (P/N 4308313), what rodent is the Control RNA isolated from?

The control RNA is total RNA isolated from a mouse lymphoma cell line.

Are there any TaqMan Endogenous Controls reagents available that can be used with rat samples?

We have TaqMan Endogenous Controls for 18S Ribosomal RNA and a kit (Cat. No. 4308313) that can be used with rat samples. The Eukaryotic 18S Ribosomal RNA Reagent is in a highly conserved area that allows this TaqMan Endogenous Control to be used for rat, human and mouse. In addition, we have newer TaqMan Gene Expression Rat Endogenous Controls, including beta glucuronidase and beta-2 microglobulin. These can be found by searching our Gene Expression Assays and specifying the species of interest as R. norvegicus and the set membership as Endogenous Controls.