Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro
AuthorsSessions, DR; Vick, MM; Fitzgerald, BP
JournalJOURNAL OF ANIMAL SCIENCE
PubMed ID
Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium ... More
Molecular cloning and characterization of porcine calcineurin-alpha subunit expression in skeletal muscle.
AuthorsDepreux FF, Scheffler JM, Grant AL, Bidwell CA, Gerrard DE
JournalJ Anim Sci
PubMed ID19897633
The calmodulin/Ca(2+)-dependent serine/threonine phophatase, calcineurin (CaN), has been implicated in controlling muscle fiber phenotype. However, little information is available concerning the expression of CaN in porcine skeletal muscle. Therefore, the porcine CaN alpha (CaN-A) was cloned by reverse transcription-PCR and its expression characterized in selected porcine skeletal muscles. We successfully ... More
Cell lines as candidate reference materials for quality control of ERBB2 amplification and expression assays in breast cancer.
AuthorsXiao Y, Gao X, Maragh S, Telford WG, Tona A
JournalClin Chem
PubMed ID19443566
BACKGROUND: Human epidermal growth factor receptor 2 (HER2) is an important biomarker whose status plays a pivotal role in therapeutic decision-making for breast cancer patients and in determining their clinical outcomes. Ensuring the accuracy and reproducibility of HER2 assays by immunohistochemistry (IHC) and by fluorescence in situ hybridization (FISH) ... More
Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro.
AuthorsSugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K, Saito S, Shimotohno KW, Suzuki T, Shimizu Y, Tobita R, Hijikata M, Takaku H, Shimotohno K
JournalJ Virol
PubMed ID19369330
Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5' untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, ... More
HIV-1 transactivator protein induction of suppressor of cytokine signaling-2 contributes to dysregulation of IFN{gamma} signaling.
AuthorsCheng SM, Li JC, Lin SS, Lee DC, Liu L, Chen Z, Lau AS
JournalBlood
PubMed ID19279332
HIV infection remains a worldwide threat. HIV-1 transactivator protein Tat is one of the retroviral proteins identified as a key immunomodulator in AIDS pathogenesis. Although the primary function of Tat is to regulate HIV-1 replication in the infected cell, it also dysregulates cytokine production resulting in perturbation of the host ... More
PURPOSE: The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in an animal model. METHODS: Retinal tissues from animals ... More
Dynamic PolyConjugates for targeted in vivo delivery of siRNA to hepatocytes.
AuthorsRozema DB, Lewis DL, Wakefield DH, Wong SC, Klein JJ, Roesch PL, Bertin SL, Reppen TW, Chu Q, Blokhin AV, Hagstrom JE, Wolff JA
JournalProc Natl Acad Sci U S A
PubMed ID17652171
Achieving efficient in vivo delivery of siRNA to the appropriate target cell would be a major advance in the use of RNAi in gene function studies and as a therapeutic modality. Hepatocytes, the key parenchymal cells of the liver, are a particularly attractive target cell type for siRNA delivery ... More
A novel one-armed anti-c-Met antibody inhibits glioblastoma growth in vivo.
AuthorsMartens T, Schmidt NO, Eckerich C, Fillbrandt R, Merchant M, Schwall R, Westphal M, Lamszus K
JournalClin Cancer Res
PubMed ID17062691
PURPOSE: Expression of the receptor tyrosine kinase c-Met and its ligand scatter factor/hepatocyte growth factor (SF/HGF) are strongly increased in glioblastomas, where they promote tumor proliferation, migration, invasion, and angiogenesis. We used a novel one-armed anti-c-Met antibody to inhibit glioblastoma growth in vivo. EXPERIMENTAL DESIGN: U87 glioblastoma cells (c-Met ... More
Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina
Infection with Equid Herpesvirus type 1 (EHV-1) leads to respiratory disease, abortion, and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A(2254)/G(2254)) in the genome region of the open reading frame 30 (ORF30), which results in an amino acid variation (N(752)/D(752)) of the EHV-1 ... More
Breed difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα
AuthorsShan, T; Wu, T; Reng, Y; Wang, Y
JournalANIMAL GENETICS
PubMed ID
Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are major novel triglyceride lipases in animals. The aim of this study was to determine if there are differences in the porcine ATGL (pATGL) and HSL genes between Jinhua pigs (a fatty breed) and Landrace pigs (a leaner breed). In addition, ... More
Expression profiling and electrophysiological studies suggest a major role for Orai1 in the store-operated Ca(2+) influx pathway of platelets and megakaryocytes
Store-operated Ca(2+) influx represents a major route by which cytosolic Ca(2+) can be elevated during platelet activation, yet its molecular identity in this cell type remains highly controversial. Using quantitative RT-PCR analysis of candidate receptor-operated cation entry pathways in human platelets, we show a 30-fold higher expression of message for ... More
Longitudinal lipid profile variations and clinical change in Alzheimer's disease dementia.
Effects of Photobiomodulation with Low-level Laser Therapy on Muscle Repair Following a Peripheral Nerve Injury in Wistar Rats.
AuthorsAndreo L,Ribeiro BG,Alves AN,Martinelli ASA,Soldera CB,Horliana ACRT,Bussadori SK,Fernandes KPS,Mesquita-Ferrari RA
JournalPhotochemistry and photobiology
PubMed ID32125691
Delivery of mesenchymal stem cells in chitosan/collagen microbeads for orthopedic tissue repair.
AuthorsWang L,Rao RR,Stegemann JP
JournalCells, tissues, organs
PubMed ID23571151
Microencapsulation and delivery of stem cells in biomaterials is a promising approach to repairing damaged tissue in a minimally invasive manner. An appropriate biomaterial niche can protect the embedded cells from the challenging environment in the host tissue, while also directing stem cell differentiation toward the desired lineage. In this ... More
Co‑inhibition of miRNA‑21 and miRNA‑221 induces apoptosis by enhancing the p53‑mediated expression of pro‑apoptotic miRNAs in laryngeal squamous cell carcinoma.
AuthorsKan X,Sun Y,Lu J,Li M,Wang Y,Li Q,Liu Y,Liu M,Tian L
JournalMolecular medicine reports
PubMed ID27035337
A Set of Active Promoters with Different Activity Profiles for Superexpressing Rhodococcus Strain.
AuthorsShemyakina AO,Grechishnikova EG,Novikov AD,Asachenko AF,Kalinina TI,Lavrov KV,Yanenko AS
JournalACS synthetic biology
PubMed ID33605147
Supplemental antioxidants do not ameliorate colitis development in HLA-B27 transgenic rats despite extremely low glutathione levels in colonic mucosa.
AuthorsSchepens MA,Vink C,Schonewille AJ,Roelofs HM,Brummer RJ,van der Meer R,Bovee-Oudenhoven IM
JournalInflammatory bowel diseases
PubMed ID21910168
Cooperative effect of the attenuation determinants derived from poliovirus sabin 1 strain is essential for attenuation of enterovirus 71 in the NOD/SCID mouse infection model.
AuthorsArita M,Ami Y,Wakita T,Shimizu H
JournalJournal of virology
PubMed ID18057246
Enterovirus 71 (EV71) is a causative agent of hand, foot, and mouth disease and is also associated with serious neurological disorders. An attenuated EV71 strain [EV71(S1-3â²)] has been established in the cynomolgus monkey infection model; this strain contains the attenuation determinants derived from the type 1 poliovirus vaccine strain, Sabin ... More
Opposite effects of interferon-β on new B and T cell release from production sites in multiple sclerosis patients.
Genetic dissociation of ethanol sensitivity and memory formation in Drosophila melanogaster.
AuthorsLaFerriere H,Guarnieri DJ,Sitaraman D,Diegelmann S,Heberlein U,Zars T
JournalGenetics
PubMed ID18430923
The ad hoc genetic correlation between ethanol sensitivity and learning mechanisms in Drosophila could overemphasize a common process supporting both behaviors. To challenge directly the hypothesis that these mechanisms are singular, we examined the learning phenotypes of 10 new strains. Five of these have increased ethanol sensitivity, and the other ... More
The expression of prolyl hydroxylase domain enzymes are up-regulated and negatively correlated with Bcl-2 in non-small cell lung cancer.
AuthorsChen S,Zhang J,Li X,Luo X,Fang J,Chen H
JournalMolecular and cellular biochemistry
PubMed ID21748337
Haplotypes of promoter and intron 1 polymorphisms in the COLIA1 gene are associated with increased risk of osteoporosis.
Macrophage migration inhibitory factor: a regulator of MMP13 and inflammation in titanium particles-stimulated air pouch in vivo.
AuthorsPan X,Mao X,Cheng T,Zhang X
JournalMolecular and cellular biochemistry
PubMed ID21625952
Human cecum content modulates production of extracellular proteins by food and probiotic bacteria.
AuthorsSánchez B,Ruiz L,Suárez A,de Los Reyes-Gavilán CG,Margolles A
JournalFEMS microbiology letters
PubMed ID22092821
Analysis of human alternative first exons and copy number variation of the GJA12 gene in patients with Pelizaeus-Merzbacher-like disease.
AuthorsRuf N,Uhlenberg B
JournalAmerican journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics
PubMed ID18521858
Dynamics of bacterial communities during solid-state fermentation using agro-industrial wastes to produce poly-γ-glutamic acid, revealed by real-time PCR and denaturing gradient gel electrophoresis (DGGE).
AuthorsYong X,Cui Y,Chen L,Ran W,Shen Q,Yang X
JournalApplied microbiology and biotechnology
PubMed ID21670980
A novel arsenical has antitumor activity toward As2O3-resistant and MRP1/ABCC1-overexpressing cell lines.
AuthorsDiaz Z,Mann KK,Marcoux S,Kourelis M,Colombo M,Komarnitsky PB,Miller WH Jr
JournalLeukemia
PubMed ID18633430
Immunological differences in intestine and rectum of Atlantic cod (Gadus morhua L.).
Transplanted donor lymphocytes infused during hematopoietic stem cell transplantation (HSCT) have been shown to cure patients with hematological malignancies. However, less is known about the effects of HSCT on metastatic solid tumors. Thus, a better understanding of the immune cells and their target antigens that mediate tumor regression is urgently ... More
Activation of innate immune defense mechanisms contributes to polyomavirus BK-associated nephropathy.
Porcine sirtuin 1 gene clone, expression pattern, and regulation by resveratrol.
AuthorsShan T,Wang Y,Wu T,Liu C,Guo J,Zhang Y,Liu J,Xu Z
JournalJournal of animal science
PubMed ID18997065
Binding-induced DNA assembly and its application to yoctomole detection of proteins.
AuthorsZhang H,Li XF,Le XC
JournalAnalytical chemistry
PubMed ID22239834
Lysophosphatidylcholine acyltransferase 1 (LPCAT1) overexpression in human colorectal cancer.
AuthorsMansilla F,da Costa KA,Wang S,Kruhøffer M,Lewin TM,Orntoft TF,Coleman RA,Birkenkamp-Demtröder K
JournalJournal of molecular medicine (Berlin, Germany)
PubMed ID18974965
The alteration of the choline metabolite profile is a well-established characteristic of cancer cells. In colorectal cancer (CRC), phosphatidylcholine is the most prominent phospholipid. In the present study, we report that lysophosphatidylcholine acyltransferase 1 (LPCAT1; NM_024830.3), the enzyme that converts lysophosphatidylcholine into phosphatidylcholine, was highly overexpressed in colorectal adenocarcinomas when ... More
Characterisation of antimony-resistant Leishmania donovani isolates: biochemical and biophysical studies and interaction with host cells.
Generation and characterization of chicken bone marrow-derived dendritic cells.
AuthorsWu Z,Rothwell L,Young JR,Kaufman J,Butter C,Kaiser P
JournalImmunology
PubMed ID19909375
Dendritic cells (DCs) are bone marrow-derived professional antigen-presenting cells. The in vitro generation of DCs from either bone marrow or blood is routine in mammals. Their distinct morphology and phenotype and their unique ability to stimulate naïve T cells are used to define DCs. In this study, chicken bone marrow ... More
Allele dependent silencing of collagen type I using small interfering RNAs targeting 3'UTR Indels - a novel therapeutic approach in osteogenesis imperfecta.
Osteogenesis imperfecta, also known as âbrittle bone diseaseâ, is a heterogeneous disorder of connective tissue generally caused by dominant mutations in the genes COL1A1 and COL1A2, encoding the α1 and α2 chains of type I (pro)collagen. Symptomatic patients are usually prescribed bisphosphonates, but this treatment is neither curative nor sufficient. ... More
Comparison of Gen-probe transcription-mediated amplification, Abbott PCR, and Roche PCR assays for detection of wild-type and mutant plasmid strains of Chlamydia trachomatis in Sweden.
AuthorsMøller JK,Pedersen LN,Persson K
JournalJournal of clinical microbiology
PubMed ID18842934
The clinical performance of two nucleic acid amplification assays targeting the cryptic plasmid and two assays targeting rRNA molecules in Chlamydia trachomatis was examined. First-catch urine samples from Malmoe, Sweden, were tested for C. trachomatis with the Abbott real-time PCR assay m2000 and an in-house PCR for the new variant ... More
Estradiol-activated estrogen receptor α does not regulate mature microRNAs in T47D breast cancer cells.
AuthorsKatchy A,Edvardsson K,Aydogdu E,Williams C
JournalThe Journal of steroid biochemistry and molecular biology
PubMed ID22079223
Ectopic KIT copy number variation underlies impaired migration of primordial germ cells associated with gonadal hypoplasia in cattle (Bos taurus).
Impaired migration of primordial germ cells during embryonic development causes hereditary gonadal hypoplasia in both sexes of Northern Finncattle and Swedish Mountain cattle. The affected gonads exhibit a lack of or, in rare cases, a reduced number of germ cells. Most affected animals present left-sided gonadal hypoplasia. However, right-sided and ... More
Transcriptional changes in Huntington disease identified using genome-wide expression profiling and cross-platform analysis.
Evaluation of transcriptional changes in the striatum may be an effective approach to understanding the natural history of changes in expression contributing to the pathogenesis of Huntington disease (HD). We have performed genome-wide expression profiling of the YAC128 transgenic mouse model of HD at 12 and 24 months of age ... More
ITCH is a putative target for a novel 20q11.22 amplification detected in anaplastic thyroid carcinoma cells by array-based comparative genomic hybridization.
Anaplastic thyroid carcinoma (ATC) is one of the most virulent of all human malignancies, with a mean survival time among patients of less than 1Â year after diagnosis. To date, however, cytogenetic information on this disease has been very limited. During the course of a program to screen a panel of ... More
Inhibitory effect of schisandrin B on free fatty acid-induced steatosis in L-02 cells.
AIM: To investigate the effects of schisandrin B (Sch B) on free fatty acid (FFA)-induced steatosis in L-02 cells. METHODS: Cellular steatosis was induced by incubating L-02 cells with a FFA mixture (oleate and palmitate at the ratio of 2:1) for 24 h. Cytotoxicity and apoptosis were evaluated by 3-(4, ... More
Normal development in mice over-expressing the intracellular domain of DLL1 argues against reverse signaling by DLL1 in vivo.
AuthorsRedeker C,Schuster-Gossler K,Kremmer E,Gossler A
JournalPloS one
PubMed ID24167636
The Notch signaling pathway mediates the direct communication between adjacent cells and regulates multiple developmental processes. Interaction of the Notch receptor with its ligands induces the liberation of the intracellular portion of Notch (NICD) referred to as regulated intramembraneous proteolysis (RIP). NICD translocates to the nucleus, and by complexing with ... More
Distinct role of Mediator tail module in regulation of SAGA-dependent, TATA-containing genes in yeast.
The evolutionarily conserved Mediator complex is required for transcription of nearly all RNA Pol II-dependent promoters, with the tail module serving to recruit Mediator to active promoters in current models. However, transcriptional dependence on tail module subunits varies in a gene-specific manner, and the generality of the tail module requirement ... More
Platelet-activating factor production in the spinal cord of experimental allergic encephalomyelitis mice via the group IVA cytosolic phospholipase A2-lyso-PAFAT axis.
AuthorsKihara Y,Yanagida K,Masago K,Kita Y,Hishikawa D,Shindou H,Ishii S,Shimizu T
JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID18802104
Prediction of drug-drug interactions from in vitro induction data: application of the relative induction score approach using cryopreserved human hepatocytes.
JournalDrug metabolism and disposition: the biological fate of chemicals
PubMed ID18519654
Detection of tumor cell-specific mRNA in the peripheral blood of patients with breast cancer—evaluation of several markers with real-time reverse transcription-PCR.
JournalInternational journal of molecular sciences
PubMed ID23299436
It is widely known that cells from epithelial tumors, e.g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic breast cancer can be detected with an array of selected tumor-marker-genes ... More
Efficient production of Hantaan and Puumala pseudovirions for viral tropism and neutralization studies.
AuthorsHiga MM,Petersen J,Hooper J,Doms RW
JournalVirology
PubMed ID22209230
DHHC2 is a protein S-acyltransferase for Lck.
AuthorsZeidman R,Buckland G,Cebecauer M,Eissmann P,Davis DM,Magee AI
JournalMolecular membrane biology
PubMed ID22034844
Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression.
AuthorsWilkinson DS,Tsai WW,Schumacher MA,Barton MC
JournalMolecular and cellular biology
PubMed ID18212064
In hepatic cells, Smad and SnoN proteins converge with p53 to repress transcription of AFP, an oncodevelopmental tumor marker aberrantly reactivated in hepatoma cells. Using p53- and SnoN-depleted hepatoma cell clones, we define a mechanism for repression mediated by this novel transcriptional partnership. We find that p53 anchors activated Smads ... More
Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase.
Th17 cells play major roles in autoimmunity and bacterial infections, yet how T cell receptor (TCR) signaling affects Th17 differentiation is relatively unknown. We demonstrate that CD4(+) T cells deficient in Itk, a tyrosine kinase required for full TCR-induced activation of PLC-γ, exhibit decreased IL-17A expression, yet relatively normal expression ... More
Mitochondrial DNA damage is sensitive to exogenous H(2)O(2) but independent of cellular ROS production in prostate cancer cells.
Polyamines critically regulate all mammalian cell growth and proliferation by mechanisms such as the repression of growth-inhibitory proteins, including JunD. Decreasing the levels of cellular polyamines stabilizes JunD mRNA without affecting its transcription, but the exact mechanism whereby polyamines regulate JunD mRNA degradation has not been elucidated. RNA-binding proteins HuR ... More
Differential growth factor regulation of N-cadherin expression and motility in normal and malignant oral epithelium.
JournalMolecular therapy : the journal of the American Society of Gene Therapy
PubMed ID22108860
The reprogramming of cord blood (CB) cells into induced pluripotent stem cells (iPSCs) has potential applications in regenerative medicine by converting CB banks into iPSC banks for allogeneic cell replacement therapy. Therefore, further investigation into novel approaches for efficient reprogramming is necessary. Here, we show that the lentiviral expression of ... More
Tight spatial and temporal control in dynamic basal to distal migration of epithelial inflammatory responses and infiltration of cytoprotective macrophages determine healing skin flap transplants in mice.
AuthorsSchürmann C,Seitz O,Klein C,Sader R,Pfeilschifter J,Mühl H,Goren I,Frank S
JournalAnnals of surgery
PubMed ID19247044
Adaptation of Porphyromonas gingivalis to microaerophilic conditions involves increased consumption of formate and reduced utilization of lactate.
AuthorsLewis JP,Iyer D,Anaya-Bergman C
JournalMicrobiology (Reading, England)
PubMed ID19684063
Porphyromonas gingivalis, previously classified as a strict anaerobe, can grow in the presence of low concentrations of oxygen. Microarray analysis revealed alteration in gene expression in the presence of 6â% oxygen. During the exponential growth phase, 96 genes were upregulated and 79 genes were downregulated 1.4-fold. Genes encoding proteins that ... More
17Beta-estradiol differentially protects cortical pericontusional zone from programmed cell death after traumatic cerebral contusion at distinct stages via non-genomic and genomic pathways.
AuthorsBao YJ,Li LZ,Li XG,Wang YJ
JournalMolecular and cellular neurosciences
PubMed ID21803156
Complete debromination of tetra- and penta-brominated diphenyl ethers by a coculture consisting of dehalococcoides and desulfovibrio species.
AuthorsLee LK,Ding C,Yang KL,He J
JournalEnvironmental science & technology
PubMed ID21859110
skNAC (skeletal Naca), a muscle-specific isoform of Naca (nascent polypeptide-associated complex alpha), is required for myofibril organization.
AuthorsLi H,Randall WR,Du SJ
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
PubMed ID19211926
Developmental validation of a real-time PCR assay for the simultaneous quantification of total human and male DNA.
AuthorsKrenke BE,Nassif N,Sprecher CJ,Knox C,Schwandt M,Storts DR
JournalForensic science international. Genetics
PubMed ID19083861
Detection of colorectal cancer cells from feces using quantitative real-time RT-PCR for colorectal cancer diagnosis.
AuthorsKoga Y,Yasunaga M,Moriya Y,Akasu T,Fujita S,Yamamoto S,Kozu T,Baba H,Matsumura Y
JournalCancer science
PubMed ID19016757
Early detection of colorectal cancer (CRC) is desired for reducing its mortality rate. Recently, the feasibility of a new method for isolating colonocytes from feces was demonstrated, followed by direct sequencing analysis for detecting colorectal cancer. In the present study, gene expression analysis was conducted using quantitative realâtime reverse transcriptaseâpolymerase ... More
NOD2-expressing bone marrow-derived cells appear to regulate epithelial innate immunity of the transplanted human small intestine.
Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
AuthorsZhang Z,Sun J,Hao L,Liu C,Ma H,Jia L
JournalPloS one
PubMed ID23840320
Among the various posttranslational modification reactions, glycosylation is the most common, and nearly 50% of all known proteins are thought to be glycosylated. In fact, changes in glycosylation readily occur in carcinogenesis, invasion and metastasis. This report investigated the modification of glycosylation mediated the invasive properties of Hca-F and Hca-P ... More
Proliferation and migration of label-retaining cells of the kidney papilla.
JournalJournal of the American Society of Nephrology : JASN
PubMed ID19762493
The kidney papilla contains a population of cells with several characteristics of adult stem cells, including the retention of proliferation markers during long chase periods (i.e., they are label-retaining cells [LRCs]). To determine whether the papillary LRCs generate new cells in the normal adult kidney, we examined cell proliferation throughout ... More
Effect of sex and age on bovine muscle and adipose fatty acid composition and stearoyl-CoA desaturase mRNA expression.
AuthorsBartoň L,Bureš D,Kott T,Rehák D
JournalMeat science
PubMed ID21640489
Remarkable expression in the colon adenocarcinoma of Hmat-Xa, a human mannosyltransferase-like gene, that is homologous to drosophila gene GC15914.
AuthorsShimono N,Nishimura Y,Kamiguchi H,Nishikawa Y
JournalBioscience, biotechnology, and biochemistry
PubMed ID21821951
A duplex real-time polymerase chain reaction assay for the simultaneous detection of long terminal repeat regions and envelope protein gene sequences of Reticuloendotheliosis virus in avian blood samples.
AuthorsSun F,Ferro PJ,Lupiani B,Kahl J,Morrow ME,Flanagan JP,Estevez C,Clavijo A
JournalJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
PubMed ID21908350
Farnesol induces the transcriptional accumulation of the Aspergillus nidulans Apoptosis-Inducing Factor (AIF)-like mitochondrial oxidoreductase.
Involvement of PML nuclear bodies in CBP degradation through the ubiquitin-proteasome pathway.
AuthorsSt-Germain JR,Chen J,Li Q
JournalEpigenetics
PubMed ID19011377
Retinoic acid modulates chromatin to potentiate tumor necrosis factor alpha signaling on the DIF2 promoter.
AuthorsWitcher M,Pettersson F,Dupéré-Richer D,Padovani A,Summers-Deluca L,Baldwin AS,Miller WH Jr
JournalNucleic acids research
PubMed ID18039708
Transcriptional activation by nuclear hormone receptors is well characterized, but their cooperation with other signaling pathways to activate transcription remains poorly understood. Tumor necrosis factor alpha (TNFα) and all-trans retinoic acid (RA) induce monocytic differentiation of acute promyelocytic leukemia (APL) cells in a synergistic manner. We used the promoter of ... More
Estradiol-sertraline synergy in ovariectomized rats.
AuthorsSell SL,Craft RM,Seitz PK,Stutz SJ,Cunningham KA,Thomas ML
JournalPsychoneuroendocrinology
PubMed ID18650020
Mice with inactivation of aryl hydrocarbon receptor-interacting protein (Aip) display complete penetrance of pituitary adenomas with aberrant ARNT expression.
Mutations in the aryl hydrocarbon receptor-interacting protein (AIP) gene have been shown to predispose to pituitary adenoma predisposition, a condition characterized by growth hormone (GH)-secreting pituitary tumors. To study AIP-mediated tumorigenesis, we generated an Aip mouse model. Heterozygous mice developed normally but were prone to pituitary adenomas, in particular to ... More
MicroRNA profiling of developing and regenerating pancreas reveal post-transcriptional regulation of neurogenin3.
AuthorsJoglekar MV,Parekh VS,Mehta S,Bhonde RR,Hardikar AA
JournalDevelopmental biology
PubMed ID17936263
Migration inhibition of mammary epithelial cells by Syk is blocked in the presence of DDR1 receptors.
AuthorsNeuhaus B,Bühren S,Böck B,Alves F,Vogel WF,Kiefer F
JournalCellular and molecular life sciences : CMLS
PubMed ID21499918
The non-receptor tyrosine kinase Syk is a well-characterized hematopoietic signal transducer, which is also expressed in non-hematopoietic cells. In epithelial cells, the function of Syk is not wholly known. It interacts with the receptor tyrosine kinase DDR1 and is frequently lost from metastatic mammary tumors. Here, using genetic tracing, we ... More
Real-time and conventional PCR detection of Liberty Link rice varieties and transgenic soy in rice sampled in the Mexican and American retail markets.
AuthorsQuirasco M,Schoel B,Chhalliyil P,Fagan J,Gálvez A
JournalAnalytical and bioanalytical chemistry
PubMed ID18668229
miRNA analysis of prostate cancer by quantitative real time PCR: comparison between formalin-fixed paraffin embedded and fresh-frozen tissue.
AuthorsLeite KR,Canavez JM,Reis ST,Tomiyama AH,Piantino CB,Sañudo A,Camara-Lopes LH,Srougi M
JournalUrologic oncology
PubMed ID19734068
Differentiation of human embryonic stem cells into osteogenic or hematopoietic lineages: a dose-dependent effect of osterix over-expression.
AuthorsKärner E,Unger C,Cerny R,Ahrlund-Richter L,Ganss B,Dilber MS,Wendel M
JournalJournal of cellular physiology
PubMed ID18932205
MicroRNA miR-7 is preferentially expressed in endocrine cells of the developing and adult human pancreas.
JournalThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
PubMed ID21430260
Laser-based tissue microdissection is an important tool for the molecular evaluation of histological sections. The technology has continued to advance since its initial commercialization in the 1990s, with improvements in many aspects of the process. More recent developments are tailored toward an automated, operator-independent mode that relies on antibodies as ... More
Drug transporter-independent liver cancer cell killing by a marine steroid methyl spongoate via apoptosis induction.
Hepatocellular carcinoma (HCC) is inherently resistant to the majority of clinical anticancer drugs. To obtain drugs that can circumvent or evade such inherent drug resistance of HCC, we investigated the effect of the marinely derived steroid methyl spongoate (MESP) on HCC cells. MESP displayed potent cell killing against a panel ... More
Two different tetracycline resistance mechanisms, plasmid-carried tet(L) and chromosomally located transposon-associated tet(M), coexist in Lactobacillus sakei Rits 9.
AuthorsAmmor MS,Gueimonde M,Danielsen M,Zagorec M,van Hoek AH,de Los Reyes-Gavilán CG,Mayo B,Margolles A
JournalApplied and environmental microbiology
PubMed ID18192429
Lactobacillus sakei is extensively used as functional starter culture in fermented meat products. One of the safety criteria of a starter culture is the absence of potentially transferable antibiotic resistance determinants. However, tetracycline-resistant L. sakei strains have already been observed. In this paper, we show that tetracycline resistance in L. ... More
High-mobility group A1 proteins enhance the expression of the oncogenic miR-222 in lung cancer cells.
AuthorsZhang Y,Ma T,Yang S,Xia M,Xu J,An H,Yang Y,Li S
JournalMolecular and cellular biochemistry
PubMed ID21656127
Identification of Trypanosoma cruzi discrete typing units (DTUs) through the implementation of a high-resolution melting (HRM) genotyping assay.
AuthorsHiguera SL,Guhl F,Ramírez JD
JournalParasites & vectors
PubMed ID23602078
BACKGROUND: Chagas disease, caused by Trypanosoma cruzi, is a geographically widespread anthropozoonosis that is considered a major public health problem in Latin America. Because this parasite presents high genetic variability, a nomenclature has been adopted to classify the parasite into six discrete typing units (DTUs): TcI, TcII, TcIII, TcIV, TcV, ... More
ULK1 regulates melanin levels in MNT-1 cells independently of mTORC1.
AuthorsKalie E,Razi M,Tooze SA
JournalPloS one
PubMed ID24066173
Melanosomes are lysosome-related organelles that serve as specialized sites of melanin synthesis and storage in melanocytes. The progression of melanosomes through the different stages of their formation requires trafficking of specific proteins and membrane constituents in a sequential manner, which is likely to deploy ubiquitous cellular machinery along with melanocyte-specific ... More
Does metformin influence the insulin-, IGF I- and IGF II-receptor gene expression and Akt phosphorylation in human decidualized endometrial stromal cells?
AuthorsCapp E,Jauckus J,von Eye Corleta H,Toth B,Strowitzki T,Germeyer A
JournalEuropean journal of obstetrics, gynecology, and reproductive biology
PubMed ID21664031
A rapid protocol for construction and production of high-capacity adenoviral vectors.
AuthorsJager L,Hausl MA,Rauschhuber C,Wolf NM,Kay MA,Ehrhardt A
JournalNature protocols
PubMed ID19373227
PCSK9 LNA antisense oligonucleotides induce sustained reduction of LDL cholesterol in nonhuman primates.
JournalMolecular therapy : the journal of the American Society of Gene Therapy
PubMed ID22108858
Proprotein convertase subtilisin/kexin type 9 (PCSK9) has emerged as a therapeutic target for the reduction of low-density lipoprotein cholesterol (LDL-C). PCSK9 increases the degradation of the LDL receptor, resulting in high LDL-C in individuals with high PCSK9 activity. Here, we show that two locked nucleic acid (LNA) antisense oligonucleotides targeting ... More
Serum cell-free DNA in renal cell carcinoma: a diagnostic and prognostic marker.
Authorsde Martino M,Klatte T,Haitel A,Marberger M
JournalCancer
PubMed ID21713763
Tethered spheroids as an in vitro hepatocyte model for drug safety screening.