TaqMan™ 基因表达Assay (FAM)
TaqMan™ 基因表达Assay (FAM)
Applied Biosystems™

TaqMan™ 基因表达Assay (FAM)

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Applied Biosystems TaqMan 基因表达Assay用于采用定量实时 PCR 分析基因表达,包括一对未标记的 PCR 引物和 TaqMan 探针,TaqMan了解更多信息
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货号反应次数
4351372S(360次反应 x 360 μL),按订单生产
4331182S(250次反应 x 250 μL),现货库存
4351368L(2900次反应 x 967 μL),按订单生产
4351370M(750次反应 x 750 μL),按订单生产
4448892XS(75次反应 x 75 μL),按订单生产
4453320XS(75次反应 x 75 μL),现货库存
货号 4351372
价格(CNY)
4,408.00
Each
立刻订购
反应次数:
S(360次反应 x 360 μL),按订单生产
Applied Biosystems TaqMan 基因表达Assay用于采用定量实时 PCR 分析基因表达,包括一对未标记的 PCR 引物和 TaqMan 探针,TaqMan 探针5’端含标记染料 (FAM) 和复合探针(Minor Groove Binder,以下简称 MGB),3’端含非荧光淬灭基团 (NFQ)。

TaqMan 基因表达Assay具有以下特点:
操作简便—只需添加 cDNA 和预混液,然后运行 qPCR—不需要生成熔解曲线
特异性—TaqMan Assay采用专有的含 MGB 的探针进行测定,这种探针比非 MGB 探针短15个碱基,从而提高Assay的特异性
灵敏度—TaqMan Assay是检测低表达或低丰度靶标的理想方法
准确度—可以检测较小的倍数变化,具有较高的定量准确度
适用范围广—可用于超过25个种属的180多万种预先设计的Assay
金标准 TaqMan qPCR 化学法—TaqMan Assay采用赛默飞世尔科技公司的生物信息学Assay流程,可确保较高的特异性和极低的交叉反应性,即使是高序列同源性的基因变异也不例外
交货快—试剂盒可在1-2天发货(见下文)

近似发货时间
盘点:北美1-2天,欧洲3-5天*
订货:北美4-6天,欧洲6-10天

TaqMan 基因表达Assay是实时 PCR 基因表达研究的金标准,具有20多年的使用经验。每种Assay均包括目标引物和序列特异性探针(已经过优化从而获得较佳的功能表现)。无需额外设计、优化或熔解曲线分析。可提供多种形式和种属,不断增加新的测定设计以满足您的研究需求。TaqMan Assay已被超过40,000份出版物引用,并且拥有超过350项专利支持。

预设计的所有 TaqMan 基因表达Assay均由 TaqMan 测定 qPCR 保证。**

推荐的预混液:TaqMan Fast Advanced 预混液

*我们热门的检测试剂盒在北美和欧洲库存,如可能,会隔夜交付。如果Assay缺货,可能还需要3–5个工作日才能发货。

**根据条款和条件而定。有关完整的详细信息,请访问 www.thermofisher.cn/taqmanguarantee

仅供科研使用。不可用于诊断程序。
规格
保真度(相对于 Taq)60 X
适用于(设备)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
环保功能绿色可持续包装
内部探针修改FAM (5'),MGB(小沟结合物)(3')
反应次数S(360次反应 x 360 μL),按订单生产
产品线TaqMan™
产品类型基因表达检测试剂盒
数量S (360 reactions/360 μL), made to order
运输条件室温
种属C. elegans, Cattle/Bovine, Dog/Canine, Drosophila, Horse/Equine, Swine/Porcine, Chicken, Zebrafish, Horse, Arabidopsis, Rabbit, Porcine, Rat, Human, Monkey, Mouse
最大浓度60X
检测方法引物-探针
形式液体
高 GC PCR 扩增效果
标签或染料FAM
PCR 方法qPCR
反应速度快速
Unit SizeEach
内容与储存
1管含20X(XS、S 和 M 大小)或60X(L 大小)预配制检测试剂(1探针和2引物)。

在 -15° C 至 -25° C 条件下储存。

常见问题解答 (FAQ)

Can I multiplex my assays if my amplicons are not all the same length?

Yes, it is possible. The amplicons do not have to be the same length in order to duplex the assays. You need to use different reporter dyes on the probes in multiplex - they should have a substantial difference in maximum emission wavelength to be effective. A classic example is to use FAM and VIC for the 2 probes. Depending on the expression of the targets, you may also need to limit the primer concentration for one of the assays. This is common when duplexing a target and endogenous control (the control probe is VIC labeled and the primer concentration is decreased so the target assay can properly compete for use of enzyme and dNTPs).

I want to order a TaqMan Gene Expression Assay that you have discontinued. Is there a way do that?

Yes, you can order it as a custom assay. Since the assay is discontinued, it can not be ordered on-line. You will have to call or email the order to genomics@appliedbiosystems.com, and include the assay ID. The assay will be synthesized once the order is received. It will be designed and priced as a Custom TaqMan Gene Expression Assay so you will need to choose the scale.

Can I use TaqMan Gene Expression Assays for a gene copy number study?

TaqMan Gene Expression Assays with “_s1” as assay ID suffix are the assays whose primers and probes are designed within a single exon, and therefore, will detect genomic DNA. However, the application of these assays in gene copy number studies has not been validated. Other gene expression assays will not detect gDNA, let alone the region of variance.

I am working on both mouse and human samples for gene expression studies. Do you have one endogenous control that I can use with both species?

The 18S rRNA is highly conserved among eukaryotes. We have several configurations of assays for this gene: P/N 4331182, Assay ID: Hs99999901_s1; PN 4319413E, 4310893E, 4333760T, 4333760F, 4308329. You can use any of these for both human and mouse samples, as well as samples from any other eukaryotic species.

Can I check the end product of a TaqMan Gene Expression Assay by running an agarose gel?

Yes, you can check the end product on an agarose gel. If you use the TaqMan Universal PCR Master Mix (including AmpErase UNG, Cat. No. 4304437), we recommend running the gel immediately after the PCR, since the trace amount of AmpErase UNG may digest the end product that contains double-stranded DNA with dUTP incorporated. The average length of amplicon ranges from 50-150 bp. The exact amplicon length is indicated on the detailed page of each assay on our website. You can get to that page by clicking on the Assay ID; the amplicon information is listed in the section "Assay Details".