TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG, 2,500 reactions - FAQs

查看更多产品信息 TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG - FAQs (4366073, 4366072, 4367846, 4364103, 4352042)

6 个常见问题解答

What is the lowest copy number that can be used with the TaqMan Fast Universal PCR Master Mix (P/N 4352042)?

The fast master mix has demonstrated detection down to 10 copies of the RNase P gene with genomic DNA. Of course, detection limits may vary depending on factors such as assay design and sample preparation.

Which TaqMan assay types can be used when using the with TaqMan Fast Universal PCR Master Mix (P/N 4352042)?

The following research assays are supported using the TaqMan Fast Universal and/or Advanced Fast PCR Master Mix: TaqMan Gene Expression Assays, Custom TaqMan Gene Expression Assays, Custom primers and probes designed from Primer Express software-designed quantitative assays.

Are there any recommendations for running primer-limited VIC dye-labeled  TaqMan Endogenous Controls using the Fast Universal PCR Master Mix such as Cat. No. 4352042?

For using primer-limited VIC dye-labeled TaqMan Endogenous Controls in a single-plex reaction, we recommend starting with an annealing/extension time of 30 sec and extension temperature of 62 ºC. For optimal performance, the recommendation is to use the TaqMan Fast Advanced Master Mix for such applications.

Are there any recommendations for running multiplex assays using the Fast Universal PCR Master Mix such as Cat. No. 4352042?

Multiplex assays applications are complex with variable performance results across different assays. Our recommendation is to use the TaqMan Fast Advanced Master Mix for such applications.

Can SNP assays be run using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

It may be technically feasible, but we have not fully validated TaqMan SNP Genotyping assays on the Applied Biosystems 7500 Fast System and therefore cannot recommend it.

What real-time PCR applications can I perform using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

You can perform experiments for Absolute Quantitation and Relative Quantitation with fast cycling. However, Allelic Discrimination (SNP) assays and plus/minus assays using internal positive controls cannot be run with fast reagents and protocols.