JournalToxicological sciences : an official journal of the Society of Toxicology
PubMed ID15673846
4-(3-pentylamino)-2,7-dimethyl-8-(2-methyl-4-methoxyphenyl)-pyrazolo-[1,5-a]-pyri midine (DMP 904) is a potent and selective antagonist of corticotropin releasing factor receptor-1 (CRF1 receptor) with an efficacious anxiolytic profile in preclinical animal models. In subchronic toxicity studies in Sprague-Dawley rats, DMP 904 produced thyroid follicular cell hypertrophy and hyperplasia, and a low incidence of follicular cell ... More
Calcineurin/nuclear factor of activated T cells and MAPK signaling induce TNF-{alpha} gene expression in pancreatic islet endocrine cells.
AuthorsLawrence MC, Naziruddin B, Levy MF, Jackson A, McGlynn K
JournalJ Biol Chem
PubMed ID21059644
Cytokines contribute to pancreatic islet inflammation, leading to impaired glucose homeostasis and diabetic diseases. A plethora of data shows that proinflammatory cytokines are produced in pancreatic islets by infiltrating mononuclear immune cells. Here, we show that pancreatic islet alpha cells and beta cells express tumor necrosis factor-alpha (TNF-alpha) and other ... More
TGF-beta regulates miR-206 and miR-29 to control myogenic differentiation through regulation of HDAC4.
AuthorsWinbanks CE, Wang B, Beyer C, Koh P, White L, Kantharidis P, Gregorevic P
JournalJ Biol Chem
PubMed ID21324893
MicroRNAs (miRs) are emerging as prominent players in the regulation of many biological processes, including myogenic commitment and skeletal muscle formation. Members of the TGF-beta family can influence the proliferation and myogenic differentiation of cells, although it is presently not clear what role miRNAs play in the TGF-beta-mediated control of ... More
Somatic cell plasticity and Niemann-Pick type C2 protein: fibroblast activation.
AuthorsCsepeggi C, Jiang M, Kojima F, Crofford LJ, Frolov A
JournalJ Biol Chem
PubMed ID21084287
A growing body of evidence points toward activated fibroblasts, also known as myofibroblasts, as one of the leading mediators in several major human pathologies including proliferative fibrotic disorders, invasive tumor growth, rheumatoid arthritis, and atherosclerosis. Niemann-Pick Type C2 (NPC2) protein has been recently identified as a product of the second ... More
Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.
AuthorsSmith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW
JournalJ Exp Med
PubMed ID19652016
The cause of Crohn's disease (CD) remains poorly understood. Counterintuitively, these patients possess an impaired acute inflammatory response, which could result in delayed clearance of bacteria penetrating the lining of the bowel and predispose to granuloma formation and chronicity. We tested this hypothesis in human subjects by monitoring responses to ... More
In vitro and in vivo enhanced generation of human A9 dopamine neurons from neural stem cells by Bcl-XL.
AuthorsCourtois ET, Castillo CG, Seiz EG, Ramos M, Bueno C, Liste I, Martinez-Serrano A
JournalJ Biol Chem
PubMed ID20106970
Human neural stem cells derived from the ventral mesencephalon (VM) are powerful research tools and candidates for cell therapies in Parkinson disease. Previous studies with VM dopaminergic neuron (DAn) precursors indicated poor growth potential and unstable phenotypical properties. Using the model cell line hVM1 (human ventral mesencephalic neural stem cell ... More
Tamoxifen increases nuclear respiratory factor 1 transcription by activating estrogen receptor beta and AP-1 recruitment to adjacent promoter binding sites.
AuthorsIvanova MM, Luken KH, Zimmer AS, Lenzo FL, Smith RJ, Arteel MW, Kollenberg TJ, Mattingly KA, Klinge CM
JournalFaseb J
PubMed ID21233487
Little is known about endogenous estrogen receptor beta (ERbeta) gene targets in human breast cancer. We reported that estradiol (E(2)) induces nuclear respiratory factor-1 (NRF-1) transcription through ERalpha in MCF-7 breast cancer cells. Here we report that 4-hydroxytamoxifen (4-OHT), with an EC(50) of ~1.7 nM, increases NRF-1 expression by recruiting ... More
Pulmonary immune responses to Propionibacterium acnes in C57BL/6 and BALB/c mice.
Propionibacterium acnes (PA) is a gram-positive anaerobic bacterium implicated as a putative etiologic agent of sarcoidosis. To characterize the pulmonary immune response to PA, C57BL/6 and BALB/c mice were intraperitoneally sensitized and intratracheally challenged with heat-killed bacteria. C57BL/6 mice challenged with PA developed a cellular immune response characterized by elevations ... More
Target genes of neuron-restrictive silencer factor are abnormally up-regulated in human myotilinopathy.
AuthorsBarrachina M, Moreno J, Juves S, Moreno D, Olive M, Ferrer I
JournalAm J Pathol
PubMed ID17823282
Myotilinopathy is a subgroup of myofibrillar myopathies caused by mutations in the myotilin gene in which there is aggregation of abnormal cytoskeletal proteins and ubiquitin. We report here on the accumulation of neuron-related proteins such as ubiquitin carboxy-terminal hydrolase L1 (UCHL1), synaptosomal-associated protein 25, synaptophysin, and alpha-internexin in aberrant protein ... More
Nuclear pregnane X receptor cross-talk with FoxA2 to mediate drug-induced regulation of lipid metabolism in fasting mouse liver.
AuthorsNakamura K, Moore R, Negishi M, Sueyoshi T
JournalJ Biol Chem
PubMed ID17267396
Upon drug activation, the nuclear pregnane X receptor (PXR) regulates not only hepatic drug but also energy metabolism. Using Pxr(-/-) mice, we have now investigated the PXR-mediated repression of lipid metabolism in the fasting livers. Treatment with PXR activator pregnenolone 16alpha-carbonitrile (PCN) down-regulated the mRNA levels of carnitine palmitoyltransferase 1A ... More
Xenobiotic-metabolizing cytochromes p450 in human white adipose tissue: expression and induction.
AuthorsEllero S, Chakhtoura G, Barreau C, Langouet S, Benelli C, Penicaud L, Beaune P, de Waziers I
JournalDrug Metab Dispos
PubMed ID20035023
Lipophilic pollutants can accumulate in human white adipose tissue (WAT), and the consequences of this accumulation are still poorly understood. Cytochromes P450 (P450s) have recently been found in rat WAT and shown to be inducible through mechanisms similar to those in the liver. The aim of our study was to ... More
RNAi Suppression of Arogenate Dehydratase1 Reveals That Phenylalanine Is Synthesized Predominantly via the Arogenate Pathway in Petunia Petals.
AuthorsMaeda H, Shasany AK, Schnepp J, Orlova I, Taguchi G, Cooper BR, Rhodes D, Pichersky E, Dudareva N
JournalPlant Cell
PubMed ID20215586
L-Phe, a protein building block and precursor of numerous phenolic compounds, is synthesized from prephenate via an arogenate and/or phenylpyruvate route in which arogenate dehydratase (ADT) or prephenate dehydratase, respectively, plays a key role. Here, we used Petunia hybrida flowers, which are rich in Phe-derived volatiles, to determine ... More
Upstream transcription factor 1 influences plasma lipid and metabolic traits in mice.
Upstream transcription factor 1 (USF1) has been associated with familial combined hyperlipidemia, the metabolic syndrome, and related conditions, but the mechanisms involved are unknown. In this study, we report validation of Usf1 as a causal gene of cholesterol homeostasis, insulin sensitivity and body composition in mouse models using several ... More
Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors.
AuthorsValencia J, Hernandez-Lopez C, Martinez VG, Hidalgo L, Zapata AG, Vicente A, Varas A, Sacedon R
JournalJ Leukoc Biol
PubMed ID19952356
Increasing evidence includes Wnt proteins inside the group of master-signaling pathways that govern immune and nonimmune differentiation systems, fundamental for normal development and homeostasis. Although their precise functions in bone marrow and thymus are still controversial, numerous studies have shown that Wnt signaling is able to control the proliferation ... More
Acute Lung Injury but not Sepsis is Associated with Increased Colony Formation by Peripheral Blood Mononuclear Cells.
AuthorsBurnham EL, Mealer M, Gaydos J, Majka S, Moss M
JournalAm J Respir Cell Mol Biol
PubMed ID19843706
""Acute lung injury (ALI) and severe sepsis are common critical illnesses associated with mobilization of bone marrow-derived cells into circulation. By identifying and determining these cellsa functional characteristics, unique prognostic biomarkers can be developed to help investigators understand mechanisms underlying the pathophysiology of these disorders. We previously demonstrated increased colony ... More
Discerning the complexity of community interactions using a Drosophila model of polymicrobial infections.
A number of human infections are characterized by the presence of more than one bacterial species and are defined as polymicrobial diseases. Methods for the analysis of the complex biological interactions in mixed infections with a large number of microorganisms are limited and do not effectively determine the contribution ... More
MicroRNA-125b is a novel negative regulator of p53.
AuthorsLe MT, Teh C, Shyh-Chang N, Xie H, Zhou B, Korzh V, Lodish HF, Lim B
JournalGenes Dev
PubMed ID19293287
The p53 transcription factor is a key tumor suppressor and a central regulator of the stress response. To ensure a robust and precise response to cellular signals, p53 gene expression must be tightly regulated from the transcriptional to the post-translational levels. Computational predictions suggest that several microRNAs are involved in ... More
EphrinB reverse signaling contributes to endothelial and mural cell assembly into vascular structures.
AuthorsSalvucci O, Maric D, Economopoulou M, Sakakibara S, Merlin S, Follenzi A, Tosato G
JournalBlood
PubMed ID19411631
EphrinB transmembrane ligands and their cognate EphB receptor tyrosine kinases regulate vascular development through bidirectional cell-to-cell signaling, but little is known about the role of EphrinB during postnatal vascular remodeling. We report that EphrinB is a critical mediator of postnatal pericyte-to-endothelial cell assembly into vascular structures. This function is ... More
Regulatory T cell suppression is potentiated by target T cells in a cell contact, IL-35- and IL-10-dependent manner.
AuthorsCollison LW, Pillai MR, Chaturvedi V, Vignali DA
JournalJ Immunol
PubMed ID19414764
Regulatory T cells (T(reg)) are believed to suppress conventional T cell (T(conv)) proliferation in vitro in a contact-dependent, cytokine-independent manner, based in part on experiments in which T(reg) and T(conv) are separated by a permeable membrane. We show that the production of IL-35, a novel inhibitory cytokine expressed by natural ... More
Is there a uniform basal endometrial gene expression profile during the implantation window in women who became pregnant in a subsequent ICSI cycle?
AuthorsAllegra A, Marino A, Coffaro F, Lama A, Rizza G, Scaglione P, Sammartano F, Santoro A, Volpes A
JournalHum Reprod
PubMed ID19542542
BACKGROUND: To understand which genes are really involved in the implantation process, we planned to study the gene basal expression profile during the window of implantation (WOI) of patients who became pregnant in a subsequent ICSI cycle. METHODS: Women attending their first ICSI cycle at ANDROS Day Surgery for ... More
An essential role for p120-catenin in Src- and Rac1-mediated anchorage-independent cell growth.
AuthorsDohn MR, Brown MV, Reynolds AB
JournalJ Cell Biol
PubMed ID19188496
p120-catenin regulates epithelial cadherin stability and has been suggested to function as a tumor suppressor. In this study, we used anchorage-independent growth (AIG), a classical in vitro tumorigenicity assay, to examine the role of p120 in a different context, namely oncogene-mediated tumorigenesis. Surprisingly, p120 ablation by short hairpin RNA completely ... More
Matrix metalloproteinase 2 and tissue inhibitors of metalloproteinases regulate human aortic smooth muscle cell migration during in vitro aging.
AuthorsVigetti D, Moretto P, Viola M, Genasetti A, Rizzi M, Karousou E, Pallotti F, De Luca G, Passi A
JournalFaseb J
PubMed ID16770011
As a direct correlation between aging and the risk of onset of vascular disease has been universally accepted, we prepared an in vitro aging model consisting in sequential passages of human aortic smooth muscle cells (AoSMC) in order to evaluate the cell behavior changes during aging. Because matrix metalloproteinases ... More
A novel vitamin D derivative activates bone morphogenetic protein signaling in MCF10 breast epithelial cells.
AuthorsLee HJ, Wislocki A, Goodman C, Ji Y, Ge R, Maehr H, Uskokovic M, Reiss M, Suh N
JournalMol Pharmacol
PubMed ID16533909
We investigated the action of 1alpha,25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)], a novel Gemini vitamin D(3) analog Ro-438-3582 [1alpha,25-dihydroxy-20S-21(3-hydroxy-3-methyl-butyl)-23-yne-26,27-hexafluorochol ecalciferol (Ro3582)], and a classic vitamin D(3) analog Ro-26-2198 [1alpha,25-dihydroxy-16,23(Z)-diene-26,27-hexafluoro-19-nor-cholecalciferol (Ro2198)] in modulating the transforming growth factor-beta (TGF-beta)/bone morphogenetic protein (BMP) system in MCF10 immortalized breast epithelial cells. We found that 1alpha,25(OH)(2)D(3), Ro3582, and ... More
Evidence for the involvement of nitric oxide in A2B receptor-mediated vasorelaxation of mouse aorta.
AuthorsAnsari HR, Nadeem A, Talukder MA, Sakhalkar S, Mustafa SJ
JournalAm J Physiol Heart Circ Physiol
PubMed ID16920807
We have investigated the role of adenosine and its analogs on vasorelaxation of mouse aorta in intact endothelium with rank order of potency as follows: 5'-N-ethylcarboxamidoadenosine (NECA) > 2-chloroadenosine > adenosine >> CGS-21680, which is consistent with the profile of A(2B)-adenosine receptor (A(2B)AR). In endothelium-intact tissues, acetylcholine produced relaxation ranging ... More
Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells.
AuthorsGee K, Angel JB, Ma W, Mishra S, Gajanayaka N, Parato K, Kumar A
JournalJ Biol Chem
PubMed ID16920714
Human immunodeficiency virus (HIV)-Tat plays an important role in virus replication and in various aspects of host immune responses, including dysregulation of cytokine production. IL-10, an anti-inflammatory cytokine, is up-regulated during the course of HIV infection representing an important pathway by which HIV may induce immunodeficiency. Here we show that ... More
Selective up-regulation of LXR-regulated genes ABCA1, ABCG1, and APOE in macrophages through increased endogenous synthesis of 24(S),25-epoxycholesterol.
Liver X receptor (LXR) activation represents a mechanism to prevent macrophage foam cell formation. Previously, we demonstrated that partial inhibition of oxidosqualene:lanosterol cyclase (OSC) stimulated synthesis of the LXR agonist 24(S),25-epoxycholesterol (24(S),25-epoxy) and enhanced ABCA1-mediated cholesterol efflux. In contrast to a synthetic, nonsteroidal LXR activator, TO-901317, triglyceride accumulation was not ... More
Silencing of bidirectional promoters by DNA methylation in tumorigenesis.
AuthorsShu J, Jelinek J, Chang H, Shen L, Qin T, Chung W, Oki Y, Issa JP
JournalCancer Res
PubMed ID16707430
CpG island methylation within promoters is known to silence individual genes in cancer. The involvement of this process in silencing gene pairs controlled by bidirectional promoters is unclear. In a screen for hypermethylated CpG islands in cancer, bidirectional promoters constituted 25.2% of all identified promoters, which matches with the genomic ... More
Histone H3 lysine 4 dimethylation signals the transcriptional competence of the adiponectin promoter in preadipocytes.
AuthorsMusri MM, Corominola H, Casamitjana R, Gomis R, Parrizas M
JournalJ Biol Chem
PubMed ID16613853
Adipogenesis is regulated by a coordinated cascade of sequence-specific transcription factors and coregulators with chromatin-modifying activities that are between them responsible for the establishment of the gene expression pattern of mature adipocytes. Here we examine the histone H3 post-translational modifications occurring at the promoters of key adipogenic genes during adipocyte ... More
Rapid onset of gene expression in lung, supportive of formation of alveolar septa, induced by refeeding mice after calorie restriction.
AuthorsMassaro D, Alexander E, Reiland K, Hoffman EP, Massaro GD, Clerch LB
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID17237152
Alveolar regenerative gene expression is unidentified partly because its onset, after a regenerative stimulus, is unknown. Toward addressing this void, we used a mouse model in which calorie restriction produces alveolar loss, and ad libitum access to food after calorie restriction induces alveolar regeneration. We selected four processes (cell replication, ... More
Optimization procedure for small interfering RNA transfection in a 384-well format.
AuthorsBorawski J, Lindeman A, Buxton F, Labow M, Gaither LA
JournalJ Biomol Screen
PubMed ID17435168
High-throughput screening of RNAi libraries has become an essential part of functional analysis in academic and industrial settings. The transition of a cell-based RNAi assay into a 384-well format requires several optimization steps to ensure the phenotype being screened is appropriately measured and that the signal-to-background ratio is above ... More
Patterns of gene expression in pig adipose tissue: transforming growth factors, interferons, interleukins, and apolipoproteins.
AuthorsHausman GJ, Barb CR, Dean RG
JournalJ Anim Sci
PubMed ID17644780
Although cDNA microarray studies have indicated the expression of unique and unexpected genes and their products in human and rodent adipose tissue, cDNA microarray studies of adipose tissue from growing pigs have not been reported. Total RNA was collected at slaughter from outer s.c. adipose tissue (OSQ), middle s.c. adipose ... More
Genome-wide functional analysis of human cell-cycle regulators.
AuthorsMukherji M, Bell R, Supekova L, Wang Y, Orth AP, Batalov S, Miraglia L, Huesken D, Lange J, Martin C, Sahasrabudhe S, Reinhardt M, Natt F, Hall J, Mickanin C, Labow M, Chanda SK, Cho CY, Schultz PG
JournalProc Natl Acad Sci U S A
PubMed ID17001007
Human cells have evolved complex signaling networks to coordinate the cell cycle. A detailed understanding of the global regulation of this fundamental process requires comprehensive identification of the genes and pathways involved in the various stages of cell-cycle progression. To this end, we report a genome-wide analysis of the human ... More
Zic3 is required for maintenance of pluripotency in embryonic stem cells.
AuthorsLim LS, Loh YH, Zhang W, Li Y, Chen X, Wang Y, Bakre M, Ng HH, Stanton LW
JournalMol Biol Cell
PubMed ID17267691
Embryonic stem (ES) cell pluripotency is dependent upon sustained expression of the key transcriptional regulators Oct4, Nanog, and Sox2. Dissection of the regulatory networks downstream of these transcription factors has provided critical insight into the molecular mechanisms that regulate ES cell pluripotency and early differentiation. Here we describe a role ... More
Enhanced airway reactivity and inflammation in A2A adenosine receptor-deficient allergic mice.
AuthorsNadeem A, Fan M, Ansari HR, Ledent C, Jamal Mustafa S
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID17293374
A(2A) adenosine receptor (A(2A)AR) has potent anti-inflammatory properties, which may be important in the regulation of airway reactivity and inflammation. Inflammatory cells that possess A(2A)AR also produce nitrosative stress, which is associated with pathophysiology of asthma, so we hypothesized that A(2A)AR deficiency may lead to increased airway reactivity and inflammation ... More
Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses.
AuthorsKleivi K, Lind GE, Diep CB, Meling GI, Brandal LT, Nesland JM, Myklebost O, Rognum TO, Giercksky KE, Skotheim RI, Lothe RA
JournalMol Cancer
PubMed ID17201907
BACKGROUND: Despite the fact that metastases are the leading cause of colorectal cancer deaths, little is known about the underlying molecular changes in these advanced disease stages. Few have studied the overall gene expression levels in metastases from colorectal carcinomas, and so far, none has investigated the peritoneal carcinomatoses ... More
Macrophages are important determinants of acute ocular HSV-1 infection in immunized mice.
AuthorsMott K, Brick DJ, van Rooijen N, Ghiasi H
JournalInvest Ophthalmol Vis Sci
PubMed ID18055810
PURPOSE: To determine the effect of macrophage depletion on herpes simplex virus type (HAV)-1 replication in the eye and on the establishment of latency in trigeminal ganglia (TG) of immunized and ocularly infected mice. METHODS: BALB/c mice were immunized with five HSV-1 glycoprotein DNA genes or were sham ... More
Cathepsin D, a lysosomal protease, regulates ABCA1-mediated lipid efflux.
To identify genes involved in the regulation of plasma high density lipoprotein (HDL) cholesterol (HDL-C) levels, patients with low HDL-C and age- and sex-matched controls (normal HDL-C) were extensively characterized. Comparative transcriptome analysis was carried out in cholesterol-loaded monocyte-derived macrophages from low HDL subjects segregated into groups with or without ... More
Role of scavenger receptor class B type I and sphingosine 1-phosphate receptors in high density lipoprotein-induced inhibition of adhesion molecule expression in endothelial cells.
AuthorsKimura T, Tomura H, Mogi C, Kuwabara A, Damirin A, Ishizuka T, Sekiguchi A, Ishiwara M, Im DS, Sato K, Murakami M, Okajima F
JournalJ Biol Chem
PubMed ID17046831
We characterized the molecular mechanisms by which high density lipoprotein (HDL) inhibits the expression of adhesion molecules, including vascular cell adhesion molecule-1 and intercellular adhesion molecule-1, induced by sphingosine 1-phosphate (S1P) and tumor necrosis factor (TNF) alpha in endothelial cells. HDL inhibited S1P-induced nuclear factor kappaB activation and adhesion molecule ... More
Human TIM-1 associates with the TCR complex and up-regulates T cell activation signals.
AuthorsBinne LL, Scott ML, Rennert PD
JournalJ Immunol
PubMed ID17371991
The T cell, Ig domain, and mucin domain-1 (TIM-1) gene is associated with Th2 T cell responses and human atopic diseases. The mechanism by which TIM-1 influences T cell responses remains unknown. We demonstrate that TIM-1 is recruited to the TCR-signaling complex via association with CD3. TIM-1 up-regulates TCR-associated signaling ... More
Virion packaging determinants and reverse transcription of SRP RNA in HIV-1 particles.
AuthorsTian C, Wang T, Zhang W, Yu XF
JournalNucleic Acids Res
PubMed ID17959647
Diverse retroviruses have been shown to package host SRP (7SL) RNA. However, little is known about the viral determinants of 7SL RNA packaging. Here we demonstrate that 7SL RNA is more selectively packaged into HIV-1 virions than are other abundant Pol-III-transcribed RNAs, including Y RNAs, 7SK RNA, U6 snRNA and ... More
Arginine methylation of the histone H3 tail impedes effector binding.
AuthorsIberg AN, Espejo A, Cheng D, Kim D, Michaud-Levesque J, Richard S, Bedford MT
JournalJ Biol Chem
PubMed ID18077460
Histone tail post-translational modification results in changes in cellular processes, either by generating or blocking docking sites for histone code readers or by altering the higher order chromatin structure. H3K4me3 is known to mark the promoter regions of active transcription. Proteins bind H3K4 in a methyl-dependent manner and aid ... More
The hedgehog signaling pathway in the mouse ovary.
AuthorsRussell MC, Cowan RG, Harman RM, Walker AL, Quirk SM
JournalBiol Reprod
PubMed ID17392501
The hedgehog (HH) signaling pathway plays an essential role in the Drosophila ovary, regulating cell proliferation and differentiation, but a role in the mammalian ovary has not been defined. Expression of components of the HH pathway in the mouse ovary and effects of altering HH signaling in vitro were ... More
A novel mechanism of tissue inhibitor of metalloproteinases-1 activation by interleukin-1 in primary human astrocytes.
AuthorsWilczynska KM, Gopalan SM, Bugno M, Kasza A, Konik BS, Bryan L, Wright S, Griswold-Prenner I, Kordula T
JournalJ Biol Chem
PubMed ID17012236
Reactive astrogliosis is the gliotic response to brain injury with activated astrocytes and microglia being the major effector cells. These cells secrete inflammatory cytokines, proteinases, and proteinase inhibitors that influence extracellular matrix (ECM) remodeling. In astrocytes, the expression of tissue inhibitor of metalloproteinases-1 (TIMP-1) is up-regulated by interleukin-1 (IL-1), which ... More
Blood leukocyte microarrays to diagnose systemic onset juvenile idiopathic arthritis and follow the response to IL-1 blockade.
AuthorsAllantaz F, Chaussabel D, Stichweh D, Bennett L, Allman W, Mejias A, Ardura M, Chung W, Smith E, Wise C, Palucka K, Ramilo O, Punaro M, Banchereau J, Pascual V
JournalJ Exp Med
PubMed ID17724127
Systemic onset juvenile idiopathic arthritis (SoJIA) represents up to 20% of juvenile idiopathic arthritis. We recently reported that interleukin (IL) 1 is an important mediator of this disease and that IL-1 blockade induces clinical remission. However, lack of specificity of the initial systemic manifestations leads to delays in diagnosis and ... More
In vitro assays fail to predict in vivo effects of regulatory polymorphisms.
AuthorsCirulli ET, Goldstein DB
JournalHum Mol Genet
PubMed ID17566082
A typical paradigm in the investigation of complex human disease is to assess the effects of cis-regulatory polymorphisms implicated in association studies on transcription in cellular expression systems. Evidence from in vitro transfection studies is often assumed to be sufficient evidence for the in vivo functional importance of a polymorphism ... More
Unique gene expression and hepatocellular injury in the lipopolysaccharide-ranitidine drug idiosyncrasy rat model: comparison with famotidine.
AuthorsLuyendyk JP, Lehman-McKeeman LD, Nelson DM, Bhaskaran VM, Reilly TP, Car BD, Cantor GH, Maddox JF, Ganey PE, Roth RA
JournalToxicol Sci
PubMed ID16415329
Rats cotreated with lipopolysaccharide (LPS) and ranitidine (RAN) but not LPS and famotidine (FAM) develop hepatocellular injury in an animal model of idiosyncratic drug reactions. Evaluation of liver gene expression in rats given LPS and/or RAN led to confirmation that the hemostatic system, hypoxia, and neutrophils (PMNs) are critical mediators ... More
Absence of immunoglobulin class switch in primary lymphomas of the central nervous system.
AuthorsMontesinos-Rongen M, Schmitz R, Courts C, Stenzel W, Bechtel D, Niedobitek G, Blumcke I, Reifenberger G, von Deimling A, Jungnickel B, Wiestler OD, Kuppers R, Deckert M
JournalAm J Pathol
PubMed ID15920162
Primary lymphomas of the central nervous system (PCNSLs) were investigated for their capacity to perform further maturation steps. We studied a series of 11 PCNSLs derived from immunocompetent patients for immunoglobulin (Ig) class switch recombination (CSR) by performing reverse transcriptase-polymerase chain reaction (RT-PCR) for transcripts of Ig constant region gene ... More
Skeletal involution by age-associated oxidative stress and its acceleration by loss of sex steroids.
AuthorsAlmeida M, Han L, Martin-Millan M, Plotkin LI, Stewart SA, Roberson PK, Kousteni S, O'Brien CA, Bellido T, Parfitt AM, Weinstein RS, Jilka RL, Manolagas SC
JournalJ Biol Chem
PubMed ID17623659
Both aging and loss of sex steroids have adverse effects on skeletal homeostasis, but whether and how they may influence each others negative impact on bone remains unknown. We report herein that both female and male C57BL/6 mice progressively lost strength (as determined by load-to-failure measurements) and bone mineral density ... More
The thymus is similar to the testis in its pattern of circadian clock gene expression.
AuthorsAlvarez JD, Sehgal A
JournalJ Biol Rhythms
PubMed ID15834108
The molecular basis for the circadian clock in mammals consists of a number of genes and proteins that form transcription-translation feedback loops. These loops result in a 24-h rhythm in the expression of mRNA and protein levels. Although the anatomical site of the central circadian clock is the SCN of ... More
A PCR-based expression signature of malignancy in follicular thyroid tumors.
AuthorsFoukakis T, Gusnanto A, Au AY, Hoog A, Lui WO, Larsson C, Wallin G, Zedenius J
JournalEndocr Relat Cancer
PubMed ID17639052
The diagnosis of follicular thyroid carcinoma (FTC) in the absence of metastasis can only be established postoperatively. Moreover, high-risk FTCs are often not identifiable at the time of diagnosis. In this study, we aimed to identify transcriptional markers of malignancy and high-risk disease in follicular thyroid tumors. The expression ... More
Overexpression of the cytotoxic T cell (CT) carbohydrate inhibits muscular dystrophy in the dyW mouse model of congenital muscular dystrophy 1A.
AuthorsXu R, Chandrasekharan K, Yoon JH, Camboni M, Martin PT
JournalAm J Pathol
PubMed ID17591965
A number of recent studies have demonstrated therapeutic effects of transgenes on the development of muscle pathology in the mdx mouse model for Duchenne muscular dystrophy, but none have been shown also to be effective in mouse models for laminin alpha2-deficient congenital muscular dystrophy (MDC1A). Here, we show that ... More
Prognostic value of CCN3 in osteosarcoma.
AuthorsPerbal B, Zuntini M, Zambelli D, Serra M, Sciandra M, Cantiani L, Lucarelli E, Picci P, Scotlandi K
JournalClin Cancer Res
PubMed ID18245529
PURPOSE: Osteosarcoma, the most common bone tumor, lacks prognostic markers that could distinguish patients before therapy and drive treatment choices. We assessed the prognostic value of CCN1, CCN2, and CCN3 genes, involved in fundamental biological processes. EXPERIMENTAL DESIGN: Expression of CCN1, CCN2, and CCN3 was measured by quantitative ... More
Application of unstable Gfp variants to the kinetic study of Legionella pneumophila icm gene expression during infection.
AuthorsBarysheva OV, Fujii J, Takaesu G, Yoshida S
JournalMicrobiology
PubMed ID18375795
An unstable type of green fluorescent protein (Gfp) tagged with a C-terminal extension, which is a target for tail-specific protease, was used as a reporter gene in Legionella pneumophila. To analyse Gfp expression in legionellae, transcriptional fusions of unstable gfp with the Legionella-specific icm (intracellular multiplication) promoters (P(icmS), P(icmT) and ... More
A global drug inhibition pattern for the human ATP-binding cassette transporter breast cancer resistance protein (ABCG2).
AuthorsMatsson P, Englund G, Ahlin G, Bergstrom CA, Norinder U, Artursson P
JournalJ Pharmacol Exp Ther
PubMed ID17616561
In this article, we explore the entire structural space of registered drugs to obtain a global model for the inhibition of the drug efflux transporter breast cancer resistance protein (BCRP; ABCG2). For this purpose, the inhibitory effect of 123 structurally diverse drugs and drug-like compounds on mitoxantrone efflux ... More
Relations of adipose tissue CIDEA gene expression to basal metabolic rate, energy restriction, and obesity: population-based and dietary intervention studies.
AuthorsGummesson A, Jernas M, Svensson PA, Larsson I, Glad CA, Schele E, Gripeteg L, Sjoholm K, Lystig TC, Sjostrom L, Carlsson B, Fagerberg B, Carlsson LM
JournalJ Clin Endocrinol Metab
PubMed ID17895319
CONTEXT: Cell death-inducing DNA fragmentation factor-alpha-like effector A (CIDEA) could be a potential target for the treatment of obesity via the modulation of metabolic rate, based on the findings that CIDEA inhibits the brown adipose tissue uncoupling process in rodents. OBJECTIVES: Our objects were to investigate the putative link between ... More
ID genes mediate tumor reinitiation during breast cancer lung metastasis.
The establishment of distant metastases depends on the capacity of small numbers of cancer cells to regenerate a tumor after entering a target tissue. The mechanisms that confer this capacity remain to be defined. Here we identify a role for the transcriptional inhibitors of differentiation Id1 and Id3 as ... More
Gene expression profiles of peripheral blood leukocytes after endotoxin challenge in humans.
AuthorsTalwar S, Munson PJ, Barb J, Fiuza C, Cintron AP, Logun C, Tropea M, Khan S, Reda D, Shelhamer JH, Danner RL, Suffredini AF
JournalPhysiol Genomics
PubMed ID16403844
To define gene expression profiles that occur during the initial activation of human innate immunity, we administered intravenous endotoxin (n = 8) or saline (n = 4) to healthy subjects and hybridized RNA from blood mononuclear cells (0, 0.5, 6, 24, 168 h) or whole blood (0, 3, 6, 24, ... More
Abnormalities of germ cell maturation and sertoli cell cytoskeleton in androgen receptor 113 CAG knock-in mice reveal toxic effects of the mutant protein.
AuthorsYu Z, Dadgar N, Albertelli M, Scheller A, Albin RL, Robins DM, Lieberman AP
JournalAm J Pathol
PubMed ID16400023
An unresolved question in the study of the polyglutamine neurodegenerative disorders is the extent to which partial loss of normal function of the mutant protein contributes to the disease phenotype. To address this, we studied Kennedy disease, a degenerative disorder of lower motor neurons caused by a CAG/glutamine expansion in ... More
5-Hydroxytryptamine (5HT) receptors in the heart valves of cynomolgus monkeys and Sprague-Dawley rats.
5-Hydroxytryptamine-2B receptor (5HT2BR) stimulation is known to cause fibroblast mitogenesis, and the mitogenic effect has been proposed to trigger valvular heart disease in humans. In this study, we used real-time polymerase chain reaction (TaqMan) to quantify transcript levels of 5HT2B, 5HT2C, and 5HT1B receptors and immunohistochemistry (IHC) to detect the ... More
Nuclear import of proinflammatory transcription factors is required for massive liver apoptosis induced by bacterial lipopolysaccharide.
AuthorsLiu D, Li C, Chen Y, Burnett C, Liu XY, Downs S, Collins RD, Hawiger J
JournalJ Biol Chem
PubMed ID15345713
Stimulation of macrophages with lipopolysaccharide (LPS) leads to the production of cytokines that elicit massive liver apoptosis. We investigated the in vivo role of stress-responsive transcription factors (SRTFs) in this process focusing on the precipitating events that are sensitive to a cell-permeant peptide inhibitor of SRTF nuclear import ... More
Molecular cloning and characterization of UDP-glucose dehydrogenase from the amphibian Xenopus laevis and its involvement in hyaluronan synthesis.
AuthorsVigetti D, Ori M, Viola M, Genasetti A, Karousou E, Rizzi M, Pallotti F, Nardi I, Hascall VC, De Luca G, Passi A
JournalJ Biol Chem
PubMed ID16418163
UDP-glucose dehydrogenase (UGDH) supplies the cell with UDP-glucuronic acid (UDP-GlcUA), a precursor of glycosaminoglycan and proteoglycan synthesis. Here we reported the cloning and the characterization of the UGDH from the amphibian Xenopus laevis that is one of the model organisms for developmental biology. We found that X. laevis UGDH (xUGDH) ... More
Regulation of Apobec3F and human immunodeficiency virus type 1 Vif by Vif-Cul5-ElonB/C E3 ubiquitin ligase.
AuthorsLiu B, Sarkis PT, Luo K, Yu Y, Yu XF
JournalJ Virol
PubMed ID16014920
The human cytidine deaminase Apobec3F (h-A3F), a protein related to the previously recognized antiviral factor Apobec3G (h-A3G), has antiviral activity against human immunodeficiency virus type 1 (HIV-1) that is suppressed by the viral protein Vif. The mechanism of HIV-1 Vif-mediated suppression of h-A3F is not fully understood. Here, we demonstrate ... More
Differential nonsense mediated decay of mutated mRNAs in mismatch repair deficient colorectal cancers.
AuthorsEl-Bchiri J, Buhard O, Penard-Lacronique V, Thomas G, Hamelin R, Duval A
JournalHum Mol Genet
PubMed ID16000315
The nonsense-mediated decay (NMD) system normally targets mRNAs with premature termination codons (PTCs) for rapid degradation. We investigated for a putative role of NMD in cancers with microsatellite instability (MSI-H cancers), because numerous mutant mRNAs containing PTC are generated in these tumors as a consequence of their mismatch repair deficiency. ... More
Selective activation of mitogen-activated protein (MAP) kinase kinase 3 and p38alpha MAP kinase is essential for cyclic AMP-dependent UCP1 expression in adipocytes.
AuthorsRobidoux J, Cao W, Quan H, Daniel KW, Moukdar F, Bai X, Floering LM, Collins S
JournalMol Cell Biol
PubMed ID15964803
The sympathetic nervous system regulates the activity and expression of uncoupling protein 1 (UCP1) through the three beta-adrenergic receptor subtypes and their ability to raise intracellular cyclic AMP (cAMP) levels. Unexpectedly, we recently discovered that the cAMP-dependent regulation of multiple genes in brown adipocytes, including Ucp1, occurred through the ... More
Adenovirus IL-13-induced airway disease in mice - A corticosteroid-resistant model of severe asthma
JournalAMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
PubMed ID
Interleukin 13 (IL-13) is considered to be a key driver of the development of airway allergic inflammation and remodeling leading to airway hyperresponsiveness (AHR). How precisely IL-13 leads to the development of airway inflammation, AHR, and mucus production is not fully understood. In order to identify key mediators downstream of ... More
Nuclear alpha NAC influences bone matrix mineralization and osteoblast maturation in vivo.
AuthorsMeury T, Akhouayri O, Jafarov T, Mandic V, St-Arnaud R
JournalMol Cell Biol
PubMed ID19884350
'Nascent-polypeptide-associated complex and coactivator alpha (alpha NAC) is a protein shuttling between the nucleus and the cytoplasm. Upon phosphorylation at residue serine 43 by integrin-linked kinase, alpha NAC is translocated to the nuclei of osteoblasts, where it acts as an AP-1 coactivator to increase osteocalcin gene transcription. To determine ... More
Down Regulation of Genes Involved in T Cell Polarity and Motility during the Induction of Heart Allograft Tolerance by Allochimeric MHC I
AuthorsLisik, W; Tejpal, N; Gong, YQ; Skelton, TS; Ganachari, M; Bremer, EG; Kloc, M; Ghobrial, RM
Journal
PubMed ID
'Background: The allochimeric MHC class I molecule [α1h1/u]-RT1. Aa that contains donor-type (Wistar Furth, WF; RT1u) epitopes displayed on recipient-type (ACI, RT1a) administered in conjunction with sub-therapeutic dose of cyclosporine (CsA) induces indefinite survival of heterotopic cardiac allografts in rat model. In vascularized transplantation models, the spleen contributes to graft ... More
A conserved E2F6-binding element in murine meiosis-specific gene promoters.
AuthorsKehoe SM, Oka M, Hankowski KE, Reichert N, Garcia S, McCarrey JR, Gaubatz S, Terada N
JournalBiol Reprod
PubMed ID18667754
'During gametogenesis, germ cells must undergo meiosis in order to become viable haploid gametes. Successful completion of this process is dependent upon the expression of genes whose protein products function specifically in meiosis. Failure to express these genes in meiotic cells often results in infertility, whereas aberrant expression in somatic ... More
A Postweaning Reduction in Circulating Ghrelin Temporarily Alters Growth Hormone (GH) Responsiveness to GH-Releasing Hormone in Male Mice But Does Not Affect Somatic Growth.
AuthorsAriyasu H, Iwakura H, Yamada G, Kanamoto N, Bando M, Kohno K, Sato T, Kojima M, Nakao K, Kangawa K, Akamizu T
JournalEndocrinology
PubMed ID20185766
'Ghrelin was initially identified as an endogenous ligand for the GH secretagogue receptor. When administrated exogenously, ghrelin stimulates GH release and food intake. Previous reports in ghrelin-null mice, which do not exhibit impaired growth nor appetite, question the physiologic role of ghrelin in the regulation of the GH/IGF-I ... More
'Our aim was to test the hypothesis that exposure to whole diesel exhaust (WDE) would enhance angiogenesis/vasculogenesis. Male apolipoprotein E-deficient mice, with either scaffold implantation subcutaneously or hindlimb ischemia, were exposed to either WDE (containing diesel exhaust particle [DEP] at a concentration of about 1 mg/m(3)) or filtered air 6 ... More
IL-1F5, -F6, -F8, and -F9: a novel IL-1 family signaling system that is active in psoriasis and promotes keratinocyte antimicrobial peptide expression.
AuthorsJohnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
JournalJ Immunol
PubMed ID21242515
'IL-1F6, IL-1F8, and IL-1F9 and the IL-1R6(RP2) receptor antagonist IL-1F5 constitute a novel IL-1 signaling system that is poorly characterized in skin. To further characterize these cytokines in healthy and inflamed skin, we studied their expression in healthy control, uninvolved psoriasis, and psoriasis plaque skin using quantitative RT-PCR and immunohistochemistry. ... More
Tissue-type plasminogen activator and the low-density lipoprotein receptor-related protein mediate cerebral ischemia-induced nuclear factor-kappaB pathway activation.
AuthorsZhang X, Polavarapu R, She H, Mao Z, Yepes M
JournalAm J Pathol
PubMed ID17717150
'Tissue-type plasminogen activator (tPA) is a serine proteinase found in the intravascular space and the central nervous system. The low-density lipoprotein receptor-related protein (LRP) is a member of the low-density lipoprotein receptor gene family found in neurons and astrocytes. Cerebral ischemia induces activation of the nuclear factor (NF)-kappaB pathway. The ... More
UCP1-independent thermogenesis in white adipose tissue of cold-acclimated Ucp1-/- mice.
AuthorsUkropec J, Anunciado RP, Ravussin Y, Hulver MW, Kozak LP
JournalJ Biol Chem
PubMed ID16914547
'Apart from UCP1-based nonshivering thermogenesis in brown adipocytes, the identity of thermogenic mechanisms that can be activated to reduce a positive energy balance is largely unknown. To identify potentially useful mechanisms, we have analyzed physiological and molecular mechanisms that enable mice, genetically deficient in UCP1 and sensitive to acute ... More
Gene expression signatures that predict radiation exposure in mice and humans.
'BACKGROUND: The capacity to assess environmental inputs to biological phenotypes is limited by methods that can accurately and quantitatively measure these contributions. One such example can be seen in the context of exposure to ionizing radiation. METHODS AND FINDINGS: We have made use of gene expression analysis of peripheral ... More
Regulation of peroxisome proliferator-activated receptor-alpha by MDM2.
'Peroxisome proliferator-activated receptor-alpha (PPARalpha) belongs to the nuclear receptor (NR) family of transcription factors and regulates lipid and glucose metabolism. Like other NRs, the regulation of gene expression by PPARalpha depends on cofactor recruitment to the transcription complex and multiple protein-protein interactions. In this study, Murine Double Minute 2 (MDM2), ... More
Genome-wide analysis of mRNA targets for Caenorhabditis elegans FBF, a conserved stem cell regulator.
AuthorsKershner AM, Kimble J
JournalProc Natl Acad Sci U S A
PubMed ID20142496
'Stem cells are essential for tissue generation during the development of multicellular creatures, and for tissue homeostasis in adults. The great therapeutic promise of stem cells makes understanding their regulation a high priority. PUF RNA-binding proteins have a conserved role in promoting self-renewal of germline stem cells. Here we use ... More
Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regulation and apoptosis inhibitor-5 as a microRNA-224-specific target.
AuthorsWang Y, Lee AT, Ma JZ, Wang J, Ren J, Yang Y, Tantoso E, Li KB, Ooi LL, Tan P, Lee CG
JournalJ Biol Chem
PubMed ID18319255
'Like other cancers, aberrant gene regulation features significantly in hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) were recently found to regulate gene expression at the post-transcriptional/translational levels. The expression profiles of 157 miRNAs were examined in 19 HCC patients, and 19 up-regulated and 3 down-regulated miRNAs were found to be associated with ... More
A Cav3.2 T-type calcium channel point mutation has splice-variant-specific effects on function and segregates with seizure expression in a polygenic rat model of absence epilepsy.
AuthorsPowell KL, Cain SM, Ng C, Sirdesai S, David LS, Kyi M, Garcia E, Tyson JR, Reid CA, Bahlo M, Foote SJ, Snutch TP, O'Brien TJ
JournalJ Neurosci
PubMed ID19144837
'Low-voltage-activated, or T-type, calcium (Ca(2+)) channels are believed to play an essential role in the generation of absence seizures in the idiopathic generalized epilepsies (IGEs). We describe a homozygous, missense, single nucleotide (G to C) mutation in the Ca(v)3.2 T-type Ca(2+) channel gene (Cacna1h) in the genetic absence epilepsy ... More
Identification of a novel nurr1-interacting protein.
AuthorsLuo Y, Xing F, Guiliano R, Federoff HJ
JournalJ Neurosci
PubMed ID18784308
'The orphan nuclear receptor Nurr1 is required for the development of ventral mesencephalic dopaminergic neurons in mice. One of the possible mechanisms that might contribute to the regulation activity of Nurr1 is through interaction with other proteins. To identify potential partners of Nurr1, we screened a yeast two-hybrid library ... More
GCN2 protein kinase is required to activate amino acid deprivation responses in mice treated with the anti-cancer agent L-asparaginase.
AuthorsBunpo P, Dudley A, Cundiff JK, Cavener DR, Wek RC, Anthony TG
JournalJ Biol Chem
PubMed ID19783659
'Asparaginase depletes circulating asparagine and glutamine, activating amino acid deprivation responses (AADR) such as phosphorylation of eukaryotic initiation factor 2 (p-eIF2) leading to increased mRNA levels of asparagine synthetase and CCAAT/enhancer-binding protein beta homologous protein (CHOP) and decreased mammalian target of rapamycin complex 1 (mTORC1) signaling. The objectives of this ... More
Biotic and abiotic stimulation of root epidermal cells reveals common and specific responses to arbuscular mycorrhizal fungi.
AuthorsGenre A, Ortu G, Bertoldo C, Martino E, Bonfante P
JournalPlant Physiol
PubMed ID19151131
'During arbuscular mycorrhizal (AM) colonization, a focal accumulation of organelles occurs in root epidermal cells, prior to fungal penetration, beneath adhering hyphopodia. This is followed by the appearance of the prepenetration apparatus (PPA), a transcellular column of cytoplasm connected to the nucleus and rich in cytoskeleton and secretory endomembranes. This ... More
Lack of ABCG2 Expression and Side Population Properties in Human Pluripotent Stem Cells
AuthorsZeng, H; Park, JW; Guo, M; Lin, G; Crandall, L; Compton, T; Wang, XF; Li, XJ; Chen, FP; Xu, RH
Journal
PubMed ID
'The multidrug transporter ABCG2 in cell membranes enables various stem cells and cancer cells to efflux chemicals, including the fluorescent dye Hoechst 33342. The Hoechst(-) cells can be sorted out as a side population with stem cell properties. Abcg2 expression in mouse embryonic stem cells (ESCs) reduces accumulation of DNA-damaging ... More
Helicobacter pylori eradication prevents progression of gastric cancer in hypergastrinemic INS-GAS mice.
AuthorsLee CW, Rickman B, Rogers AB, Ge Z, Wang TC, Fox JG
JournalCancer Res
PubMed ID18441088
'Helicobacter pylori infection results in chronic gastritis, which may progress to gastric cancer. In this study, we investigated the efficacy of H. pylori eradication in preventing the progression of gastritis to gastric cancer in H. pylori-infected transgenic INS-GAS mice. H. pylori infection induced severe dysplasia and gastric cancer classified as ... More
Splice variants of Enigma homolog, differentially expressed during heart development, promote or prevent hypertrophy.
AuthorsYamazaki T, Walchli S, Fujita T, Ryser S, Hoshijima M, Schlegel W, Kuroda S, Maturana AD
JournalCardiovasc Res
PubMed ID20097676
'AIMS: Proteins with a PDZ (for PSD-95, DLG, ZO-1) and one to three LIM (for Lin11, Isl-1, Mec-3) domains are scaffolding sarcomeric and cytoskeletal elements that form structured muscle fibres and provide for the link to intracellular signalling by selectively associating protein kinases, ion channels, and transcription factors with the ... More
Protective role of 17 beta -estradiol against the development of Helicobacter pylori-induced gastric cancer in INS-GAS mice.
AuthorsOhtani M, Garcia A, Rogers AB, Ge Z, Taylor NS, Xu S, Watanabe K, Marini RP, Whary MT, Wang TC, Fox JG
JournalCarcinogenesis
PubMed ID17724378
'The incidence of gastric cancer is higher in men than women. Epidemiological studies suggest that female hormones reduce gastric cancer risk. We examined the effect of ovarian-dependent female hormones on Helicobacter pylori-induced gastric cancer in hypergastrinemic INS-GAS mice. Male and female sexually intact or ovariectomized (OVX) mice were ... More
Polymeric linear Peptide chimeric vaccine-induced antimalaria immunity is associated with enhanced in vitro antigen loading.
AuthorsSilva-Flannery LM, Cabrera-Mora M, Dickherber M, Moreno A
JournalInfect Immun
PubMed ID19237530
'Immunization of mice with Plasmodium berghei or Plasmodium yoelii synthetic linear peptide chimeras (LPCs) based on the circumsporozoite protein protects against experimental challenge with viable sporozoites. The immunogenicity of LPCs is significantly enhanced by spontaneous polymerization. To better understand the antigenic properties of polymeric antimalarial peptides, we studied the immune ... More
Cloning and molecular characterization of R2R3-MYB and bHLH-MYC transcription factors from Citrus sinensis
AuthorsCultrone, A; Cotroneo, PS; Recupero, GR
Journal
PubMed ID
'Members of the MYB and MYC family regulate the biosynthesis of phenylpropanoids in several plant species. Two sequences, called CsMYB8 and CsMYC2, were identified from Citrus sinensis, and both the cDNA and the genomic clones were isolated and characterized from the flesh of common and blood oranges. Analysis by real-time ... More
PemK toxin of Bacillus anthracis is a ribonuclease: an insight into its active site, structure, and function.
AuthorsAgarwal S, Mishra NK, Bhatnagar S, Bhatnagar R
JournalJ Biol Chem
PubMed ID20022964
'Bacillus anthracis genome harbors a toxin-antitoxin (TA) module encoding pemI (antitoxin) and pemK (toxin). This study describes the rPemK as a potent ribonuclease with a preference for pyrimidines (C/U), which is consistent with our previous study that demonstrated it as a translational attenuator. The in silico structural modeling of the ... More
REDD1 is a major target of testosterone action in preventing dexamethasone-induced muscle loss.
AuthorsWu Y, Zhao W, Zhao J, Zhang Y, Qin W, Pan J, Bauman WA, Blitzer RD, Cardozo C
JournalEndocrinology
PubMed ID20032058
'Glucocorticoids are a well-recognized and common cause of muscle atrophy that can be prevented by testosterone. However, the molecular mechanisms underlying such protection have not been described. Thus, the global effects of testosterone on dexamethasone-induced changes in gene expression were evaluated in rat gastrocnemius muscle using DNA microarrays. Gene expression ... More
Abscisic acid regulates root hydraulic conductance via aquaporin expression modulation in Nicotiana tabacum
AuthorsMahdieh, M; Mostajeran, A
Journal
PubMed ID
'Abscisic acid (ABA) modifies the hydraulic properties of roots by increasing root water flux. The effects of ABA on aquaporin content and root hydraulic conductance are controversial. We addressed these effects via a combination of experiments. Tobacco (Nicotiana tabacum) plants were grown hydroponically, and ABA (1 μM) was exogenously applied ... More
Expression of a non-DNA-binding isoform of Helios induces T-cell lymphoma in mice.
AuthorsZhang Z, Swindle CS, Bates JT, Ko R, Cotta CV, Klug CA
JournalBlood
PubMed ID17110463
'Helios is a zinc-finger protein belonging to the Ikaros family of transcriptional regulators. It is expressed, along with Ikaros, throughout early stages of thymocyte development where it quantitatively associates with Ikaros through C-terminal zinc-finger domains that mediate heterodimerization between Ikaros family members. To understand the role of Helios in T-cell ... More
Upper airway collapse and reopening induce inflammation in a sleep apnoea model.
AuthorsAlmendros I, Carreras A, Ramirez J, Montserrat JM, Navajas D, Farre R
JournalEur Respir J
PubMed ID18448490
'The upper airway of obstructive sleep apnoea patients is subjected to recurrent negative pressure swings promoting its collapse and reopening. The aim of the present study was to ascertain whether this mechanical stress induces upper airway inflammation in a rat model. The upper airway of Sprague-Dawley rats was subjected to ... More
Interleukin-10 promotes pathological angiogenesis by regulating macrophage response to hypoxia during development.
AuthorsDace DS, Khan AA, Kelly J, Apte RS
JournalPlos One
PubMed ID18852882
'Aberrant angiogenesis in the eye is the most common cause of blindness. The current study examined the role of interleukin-10 (IL-10) in ischemia-induced pathological angiogenesis called neovascularization during postnatal development. IL-10 deficiency resulted in significantly reduced pathological retinal angiogenesis. In contrast to the choroicapillaris where IL-10 interferes with macrophage influx, ... More
Identification of hypoxia-inducible factor-1 alpha as a novel target for miR-17-92 microRNA cluster.
AuthorsTaguchi A, Yanagisawa K, Tanaka M, Cao K, Matsuyama Y, Goto H, Takahashi T
JournalCancer Res
PubMed ID18632605
'MicroRNAs (miRNAs) are a distinct class of small noncoding RNAs that posttranscriptionally repress expression of target genes through imperfect base pairing with the 3'' untranslated region. We previously reported amplification and overexpression of the miR-17-92 miRNA cluster at 13q31.3 in lung cancers, as well as growth inhibition by treatment with ... More
The Ron receptor tyrosine kinase negatively regulates mammary gland branching morphogenesis
AuthorsMeyer, SE; Zinser, GM; Stuart, WD; Pathrose, P; Waltz, SE
Journal
PubMed ID
'The Ron receptor tyrosine kinase is expressed in normal breast tissue and is overexpressed in approximately 50% of human breast cancers. Despite the recent studies on Ron in breast cancer, nothing is known about the importance of this protein during breast development. To investigate the functional significance of Ron in ... More
Glucose regulates ghrelin, neuropeptide Y, and the GH/IGF-I axis in the tilapia, Oreochromis mossambicus
'In general, a fish''s ability to clear glucose is sluggish in relation to mammals, which has lead to the idea that fish are glucose intolerant. It has been reported that circulating glucose levels do fluctuate in response to environmental challenges. Recent reports suggest that glucose may function as a metabolic ... More
Mitochondrial abnormalities in spinal and bulbar muscular atrophy.
'Spinal and bulbar muscular atrophy (SBMA) is a motor neuron disease caused by polyglutamine expansion mutation in the androgen receptor (AR). We investigated whether the mutant protein alters mitochondrial function. We found that constitutive and doxycycline-induced expression of the mutant AR in MN-1 and PC12 cells, respectively, are associated with ... More
Endocardial cells are a distinct endothelial lineage derived from Flk1+ multipotent cardiovascular progenitors
'Identification of multipotent cardiac progenitors has provided important insights into the mechanisms of myocardial lineage specification, yet has done little to clarify the origin of the endocardium. Despite its essential role in heart development, characterization of the endocardial lineage has been limited by the lack of specific markers of this ... More
Neuroinflammatory response to lipopolysaccharide is exacerbated in mice genetically deficient in cyclooxygenase-2.
AuthorsAid S, Langenbach R, Bosetti F
JournalJ Neuroinflammation
PubMed ID18489773
'BACKGROUND: Cyclooxygenases (COX) -1 and -2 are key mediators of the inflammatory response in the central nervous system. Since COX-2 is inducible by inflammatory stimuli, it has been traditionally considered as the most appropriate target for anti-inflammatory drugs. However, the specific roles of COX-1 and COX-2 in modulating ... More