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Thermo Scientific™
SurePac™ Oligo RP MDi™ HPLC Columns
SurePac Oligo RP MDI is a robust reversed-phase column designed for oligonucleotide analysis by HPLC and LC-MS. It delivers high recovery, bioinert column hardware, and reproducible separations, enabling reliable profiling of oligonucleotides of any size.
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| Catalog Number | Diameter (Metric) | Length (Metric) |
|---|---|---|
| 43712-022132 | 2.1 mm | 20 mm |
| 43712-052132 | 2.1 mm | 50 mm |
| 43712-102132 | 2.1 mm | 100 mm |
| 43712-050332 | 300 μm | 50 mm |
| 43712-0510032 | 10 mm | 50 mm |
| 43712-0521232 | 21.2 mm | 50 mm |
Catalog number 43712-022132
Price (USD)
-
Diameter (Metric):
2.1 mm
Length (Metric):
20 mm
Thermo Scientific™ SurePac™ Oligo RP MDi™ HPLC columns deliver high-resolution, reproducible separations for therapeutic oligonucleotides and nucleic acids across R and D and QC workflows. Built on monodisperse, supermacroporous polymeric packing with bio-inert hardware, they provide stable performance even under elevated pH and temperature conditions, resolving truncation impurities while supporting large oligos and intact nucleic acids for scalable LC-MS and LC-UV analysis.
- Purpose-built for oligonucleotide RP separations
- Optimized for DNA and RNA oligonucleotides, including modified sequences, delivering consistent retention and selectivity across short and long oligos.
- Bioinert, metal-free fluid path
- Reduces metal/oligonucleotide interactions to improve recovery, minimize adsorption, and deliver cleaner peak shapes for sensitive analyses.
- Optimized for ion-pair RP LC-MS
- Fully compatible with common ion-pairing reagents such as HFIP/TEA, enabling sensitive, reproducible LC-MS detection.
- High recovery of long and modified oligonucleotides
- High resolution power for oligonucleotides spanning over a wide size range from small to (ultra) large
- Supports efficient analysis of long, hydrophobic, and chemically complex oligos without compromising signal or resolution.
- Robust stationary phase for demanding methods
- Maintains stable performance under high organic content and elevated temperatures, supporting fast gradients and long column lifetimes.
- Excellent peak shape and impurity resolution
- Delivers sharp, symmetrical peaks and reliable separation of closely related species, including impurities and sequence variants.
- Reproducible, manufacturing-controlled performance
- Ensures consistent results across columns and batches, simplifying method transfer and routine use.
- Flexible for development and routine workflows
- Suitable for method development, impurity profiling, and QC, reducing the need for multiple column chemistries.
- Compatible with UHPLC and HPLC systems
- Designed for seamless integration into modern LC platforms and existing laboratory setups.
- Built for confident bioanalytical results
- Improves robustness, reduces variability, and supports reliable data generation in oligonucleotide workflows.
Specifications
Column FormatAnalytical
Flow Rate0.2 to 1 mL/min.
Max. Pressure6500 psi (448 bar)
Packing MaterialPolymeric
Stationary PhasePoly-DVB
Temperature5°C to 90°C
Diameter (Metric)2.1 mm
Length (Metric)20 mm
Particle Size2.5 μm
Pore SizeProprietary Wide Pore
pH2 to 12
Unit SizeEach