TaqMan™ Non-coding RNA Assay
TaqMan™ Non-coding RNA Assay
Applied Biosystems™

TaqMan™ Non-coding RNA Assay

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Applied Biosystems TaqMan Noncoding RNA assays 用于 RNA 表达的定量实时荧光定量 PCR了解更多信息
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货号数量
4426961S (360 rxns), made to order
4426962M (750 rxns), made to order
4426963L (2900 rxns), made to order
货号 4426961
价格（CNY)
4,542.00
Each
立刻订购
数量:
S (360 rxns), made to order
Applied Biosystems TaqMan Noncoding RNA assays 用于 RNA 表达的定量实时荧光定量 PCR 分析，包括一对未标记的 PCR 引物和一对末端5’标记染料 (FAM) 及末端3’ 存在小沟结合物 (MGB) 和非荧光染淬灭基团 (NFQ) 的 TaqMan 探针。

特征包括：
•使用方便—仅需添加 cDNA 和预混液，即可运行 qPCR— 无需熔解曲线
•特异性— TaqMan 检测使用专有的含 MGB 探针（比15碱基短，且短于非 MGB 探针），从而提高了检测特异性
•灵敏— TaqMan 检测是测量低表达水平或低丰度靶标的较佳方法
•准确—识别较小倍数变化，且在定量方面具有高准确性
•金标准 TaqMan qPCR 化学方法—采用赛默飞世尔科技公司的生物信息学检测设计产品组合进行 TaqMan 测定，以帮助确保高特异性和较小的交叉反应性，即使对于具有高序列同源性的基因变异亦是如此

大致运输时间
定制：4–6欧洲 6–10 天

TaqMan 基因表达检测是实时荧光定量 PCR 基因表达研究中的金标准，建立在超过 20 年经验的基础上。每种测定均包括目标引物和序列特异性探针（已经过优化从而获得较佳的功能表现）。无需额外设计、优化或熔解曲线分析。可提供多种形式和种属，不断增加新的测定设计以满足您的研究需求。TaqMan 试剂盒已被超过40,000份出版物引用，并且拥有超过350项专利支持。我们所有的预设计 TaqMan Gene Expression assays 均可见于 TaqMan ssays qPCR 保证书中。 *

推荐预混液：TaqMan Fast Advanced 预混液

* 受条款和条件约束。有关完整详细信息，请访问 www.thermofisher.cn/taqmanguarantee。

仅供科研使用。不可用于诊断程序。
规格
最大浓度60 X
环保功能绿色可持续包装
反应次数360 次反应
PCR 方法qPCR
产品线TaqMan™
产品类型非编码 RNA 测定试剂盒
数量S (360 rxns), made to order
反应速度快速
运输条件室温
检测方法引物-探针
产品规格管装
Unit SizeEach
内容与储存
1 个含 20X（S 和 M 规格）或 60X（L 规格）预配制检测试剂（1 种探针和 2 种引物）混合物的管。

在 -15 至 -25°C 下储存。

常见问题解答 (FAQ)

Did you take into account sequence homology when you developed the TaqMan Gene Expression Assays product?

During the TaqMan Gene Expression Assays product (including TaqMan Gene Expression Assays and Custom Plus TaqMan RNA Assays) design process, we take the following steps to avoid regions of ambiguity: 1. We BLAST the primer and probe designs against transcript databases to ensure specificity to ensure the chosen assay detects only transcript(s) from the gene of interest. 2. We BLAST primer and probe designs against genome databases in order to avoid assays that detect pseudo-genes or genomic DNA.

What region of my gene of interest does the TaqMan Gene Expression Assay product cover?

You can view TaqMan Gene Expression Assay product coverage on the Assay details page. To do this, search for your gene of interest, for example "Cox".  On your results page, select the "Assay ID" for a particular TaqMan Gene Expression Assay; for example: Hs00187909_m1.  The resulting page is the assay details page and will list regions covered by the TaqMan Gene Expression Assay.

When preparing the TaqMan or Custom TaqMan Gene Expression Assays, what is the recommended reaction volume?

When preparing the TaqMan or Custom TaqMan Gene Expression Assays, the recommended and supported volume is 20-50 µL for a 96-well standard plate set-up; 10-20 µL for a 96-well fast plate set-up and 5-10 µL for a 384-well set-up. For more information, please consult the TaqMan Gene Expression Assays and Custom TaqMan Gene Expression Assays protocols.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

How much cDNA is recommended per TaqMan or Custom TaqMan Gene Expression reaction?

For optimal performance of the TaqMan and Custom TaqMan Gene Expression Assays, use 1 to 100 ng of cDNA per 50 µL total reaction volume. For more information, please consult the TaqMan Gene Expression Assay protocol and Custom TaqMan Gene Expression Assay protocol.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

Can I use SYBR Green I dye in conjunction with the TaqMan Gene Expression Assays or Custom TaqMan Gene Expression Assays?

No, SYBR Green dye I is not compatible with the TaqMan Gene Expression Assays or Custom TaqMan Gene Expression Assays. TaqMan Gene Expression Assays or Custom TaqMan Gene Expression Assays contain TaqMan MGB probes combined with primers at non-limiting concentrations. The probes already have detection dyes conjugated to them, and SYBR Green is not needed. For more information, please consult the protocol of TaqMan Gene Expression Assays and Custom TaqMan Gene Expression Assays.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

View all TaqMan™ Non-coding RNA Assay FAQs