Custom Plus TaqMan ™RNA 测定,FAM
Custom Plus TaqMan ™RNA 测定,FAM
Applied Biosystems™

Custom Plus TaqMan ™RNA 测定,FAM

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Applied Biosystems Custom Plus TaqMan RNA Assay是我们用于实时荧光定量 PCR 的预设计 TaqMan了解更多信息
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货号数量
4441118L (2900 reactions), made to order
4441117M (750 reactions), made to order
4441114S (360 reactions), made to order
货号 4441118
价格(CNY)
15,360.00
Each
立刻订购
数量:
L (2900 reactions), made to order
Applied Biosystems Custom Plus TaqMan RNA Assay是我们用于实时荧光定量 PCR 的预设计 TaqMan Assay的个性化定制版本。Custom Plus TaqMan RNA Assay包括一对未标记的 PCR 引物和 5’ 端染色标记 (FAM) 的 TaqMan 探针,以及 3’ 端小沟结合物 (MGB) 和非荧光淬灭剂 (NFQ)。

特点包括:
易于使用—仅添加 cDNA 和预混液运行 qPCR—无需熔点曲线
特异性—TaqMan 分析使用专利含 MGB 的探针,该探针比非 MGB 探针少 15 个碱基,提高了检测的特异性。
灵敏性—TaqMan 分析是测量低表达水平或低丰度目标的理想方法
准确性—识别小倍数变化病具有高准确性定量
定制含量—设计易于检测超过 20 个物种
订制设计T基于强大的生信能力和专业算法,生成数百万个Assay,并被视为业内 qPCR 设计的基准

大致运输时间
定制:4–6欧洲 6–10 天

TaqMan 基因表达Assay是实时荧光定量 PCR 基因表达研究中的金标准,建立在超过 20 年经验的基础上。每种Assay均包括目标引物和序列特异性探针(已经过优化从而获得较佳的功能表现)。无需额外设计、优化或熔解曲线分析。可提供多种形式和种属,不断增加新的Assay以满足您的研究需求。TaqMan Assay已被超过 40,000 篇出版物引用,并且获得超过 350 项专利的支持。

推荐的预混液:TaqMan Fast Advanced预混液

仅供科研使用。不可用于诊断程序。
规格
适用于(设备)7500 系统、7500 Fast 系统、7900HT 系统、StepOne™、StepOnePlus™、ViiA™ 7 系统、QuantStudio™ 3、QuantStudio™ 5、QuantStudio™ 6 Flex、QuantStudio™ 7 Flex、QuantStudio 6 Pro、QuantStudio 7 Pro、QuantStudio™ 12k Flex
环保功能绿色可持续包装
反应次数2900 reactions, made to order
产品线TaqMan™
数量L (2900 reactions), made to order
运输条件室温
最大浓度60X
Unit SizeEach
内容与储存
1 个含 20X(S 和 M 规格)或 60X(L 规格)预配制检测试剂(1 种探针和 2 种引物)混合物的管。

在 -15 至 -25°C 下储存。

常见问题解答 (FAQ)

Can I set up and store the TaqMan Fast Universal PCR Master Mix at room temperature if the instrument is in use?

For optimal results, we recommend to run the reaction plate right after setting up the reaction. If a reaction plate cannot be run within 2 hours of completing the reaction setup, then freeze/refrigerate the reaction plate until it can be loaded and run on the fast PCR instrument platform.

Can I run a reaction made with the standard TaqMan Universal PCR Master Mix in fast thermal cycling mode?

No, we do not recommend this approach. Since the standard TaqMan Universal PCR Master Mix is not optimized for running with fast thermal cycling, assay results will be significantly compromised.

Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

Can I compare performance of assays between the TaqMan Fast Universal PCR Master Mix compare and TaqMan Universal PCR Master Mix?

In general, assays have been found to perform comparably with these reagents, but they may not provide exactly identical results.

What are the storage conditions and the shelf life of the TaqMan Fast Universal PCR Master Mix?

We recommend a storage temperature of 4C and the shelf life is 9 months from the manufacturing date.