POROS™ CaptureSelect™ hGH 亲和柱，4.6 x 50 mm
POROS™ CaptureSelect™ hGH 亲和柱，4.6 x 50 mm
Thermo Scientific™

POROS™ CaptureSelect™ hGH 亲和柱，4.6 x 50 mm

Thermo Scientific™ POROS™ CaptureSelect™ hGH HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.
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货号数量
44851651 个色谱柱
货号 4485165
价格（CNY)
33,357.00
Each
添加至购物车
数量:
1 个色谱柱
价格（CNY)
33,357.00
Each
添加至购物车

Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS CaptureSelect hGH HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands. The combination allows three-minute quantitation of human growth factor (hGH).

  • Broad utility—POROS A, G, and CaptureSelect affinity ligands address most biological drug classes
  • Rapid—POROS resins offer fast quantitation or purification of immunoglobulins, fusion proteins
  • Hands-off operation—HPLC method can be automated for streamlined operation
  • Robust—allows for high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity

The combination of CaptureSelect hGH affinity ligand and POROS 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid titer determination and small-scale purification of hGH.

POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and allows for an eluted product that is highly purified and suitable for further analysis, including mass spectrometry.

Automation

POROS affinity chromatography columns are suitable for use with HPLC systems, which allows for the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles

Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime can result in strong re-use performance. POROS affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和、生长激素亲和配体、人特异性
最大洗脱体积29 根色谱柱体积
最小洗脱体积14 根色谱柱体积
高通量能力自动化方案
基质POROS
最大压力2600 psi，180 bar
数量1 个色谱柱
固定相CaptureSelect™ hGH
靶标分子蛋白质
容积（公制）0.8 mL
粒径20 微米
产品线CaptureSelect，POROS
类型亲和柱
Unit SizeEach
内容与储存
层析柱包括 EZ grip 配件和层析柱检测证书。
在 2–8°C 下储存。切勿冷冻。

常见问题解答 (FAQ)

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all POROS™ CaptureSelect™ hGH 亲和柱，4.6 x 50 mm FAQs