AminoLink™ 固定试剂盒
AminoLink™ 固定试剂盒
Thermo Scientific™

AminoLink™ 固定试剂盒

AminoLink 固定试剂盒含有 AminoLink 偶联树脂以及用于进行 5 次蛋白固定的相关试剂,预期得率达到 2 至 20 mg了解更多信息
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货号数量
448905 个色谱柱试剂盒
货号 44890
价格(CNY)
6,052.00
Each
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数量:
5 个色谱柱试剂盒
请求批量或定制报价
价格(CNY)
6,052.00
Each
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AminoLink 固定试剂盒含有 AminoLink 偶联树脂以及用于进行 5 次蛋白固定的相关试剂,预期得率达到 2 至 20 mg 蛋白/柱。AminoLink 偶联树脂是一种已经过醛基活化的交联 4% 微珠状琼脂糖,通过伯胺实现抗体和其他蛋白的共价固定。

AminoLink 偶联树脂的特点:

AminoLink 偶联树脂 — 醛活化交联 4% 微珠状琼脂糖
• 非常适用于抗体和其他蛋白 — 通过伯胺 (-NH2) 固定分子
灵活的偶联条件 — 可在多种 pH 值 (4-10) 和缓冲液(PBS 或其他含有或不含有机溶剂的非胺缓冲液)条件下实现高效 (>85%) 偶联
稳定、永久固定 — 偶联反应在树脂和配体之间产生稳定、防渗漏的仲胺键
优于 CNBr-活化琼脂糖的固定化 — 化学键更为稳定且不带电荷,在亲和纯化步骤中产生更少的非特异性结合
多功能且可重复使用 — 制备的亲和树脂可适应色谱柱和批次亲和技术,并可根据蛋白结合相互作用为常规应用提供可重复使用的树脂

蛋白和含有伯胺的其他分子可以共价连接(固定)到 AminoLink 树脂上,从而使色谱柱可用于亲和纯化。醛基与伯胺形成稳定的仲胺键,如存在于赖氨酸 (K) 残基的侧链中,该残基通常在蛋白中丰度高且易于进入蛋白。蛋白固定化后,制备的亲和树脂可用于涉及固定化蛋白结合相互作用的各种批次和柱亲和纯化方法。树脂和连结在亲和色谱分析中通常使用的大多数结合和洗脱条件下是稳定的,使得制备好的树脂可用于多次亲和纯化程序。

AminoLink 树脂固定反应涉及在醛类和胺自然形成席夫基体连结,并可使用温和还原剂培养对其进行后续稳定。整个偶联反应(称为还原胺化)发生在简单非胺缓冲液(如 PBS)中,在 4 至 6 小时内完成。与各种抗体和常见蛋白的偶联效率通常大于 85%,每毫升树脂可固定 1 至 20 mg 蛋白。

相关产品
AminoLink™ 偶联树脂
AminoLink™ 还原剂
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和,琼脂糖树脂
产品规格离心柱
数量5 个色谱柱试剂盒
固定相醛类
足够用于5 次蛋白固定
靶标抗体
产品线AminoLink™
类型固定试剂盒
Unit SizeEach
内容与储存
足够用于:固定 2 至 20 mg 蛋白/柱
• AminoLink 柱,2 mL,5 个柱
• AminoLink 偶联缓冲液(pH 值 7.0),250 mL
• 淬灭缓冲液,50 mL
• 洗涤缓冲液,240 mL
• 氰基硼氢化钠溶液,5 M,0.5 mL
• 柱配件

在 4°C 下储存

常见问题解答 (FAQ)

Does sodium azide interfere with AminoLink Support coupling?

No, the gel is stored in sodium azide with no loss in activity. Azide is not a primary amine and is rather unreactive.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between AminoLink Coupling Resin and NHS-Activated Agarose?

Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between AminoLink and AminoLink Plus supports?

AminoLink Plus Coupling Resin is activated at a higher level and has higher flow rates than the original AminoLink Coupling Resin, resulting in higher capacity and faster purification. AminoLink Supports can be used to immobilize any molecule with a primary amine.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do your AminoLink Coupling Resin and AminoLink Plus Coupling Resin work?

AminoLink and AminoLink Plus Supports are activated with aldehyde groups which will react with primary amines to form Schiff bases, which are reduced to stable, non-reversible secondary amines. Coupling efficiency often exceeds 85% with this support.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.