AminoLink™ 还原剂

Thermo Scientific AminoLink 还原剂（氰基硼氢化钠）促进含醛分子和含胺分子之间的稳定键形成，从而实现蛋白和其他分子的有效标记、偶联和固定。羰基（如醛、酮和乙二醛了解更多信息

货号	数量
44892	2 x 1 g

货号 44892

价格（CNY)

1,028.00

添加至购物车

2 x 1 g

请求批量或定制报价

价格（CNY)

1,028.00

添加至购物车

Thermo Scientific AminoLink 还原剂（氰基硼氢化钠）促进含醛分子和含胺分子之间的稳定键形成，从而实现蛋白和其他分子的有效标记、偶联和固定。羰基（如醛、酮和乙二醛）与氨基自发反应，形成与其游离形式相平衡的席夫碱中间体。相互作用取决于 pH 值，在酸性条件下更有效，特别是在高 pH 值下。向形成席夫碱的反应中添加 AminoLink 还原剂会导致不稳定席夫碱中间体完全还原为化学稳定键。与硼氢化钠不同，氰基硼氢化钠具有足够的温和性，可避免醛被反向还原为非反应性羟基。

羰基与伯胺基的总体反应（使用氰基硼氢化钠）称为还原性胺化。通过还原性胺化将含胺生物分子固定在含醛固相支持物上，用于生成抗体和其他分子亲和纯化的基质。为通过还原性胺化作用偶联糖蛋白，必须使用偏高碘酸钠氧化多糖基团中的糖，以生成反应性醛基。用偏高碘酸钠处理糖蛋白通常会生成足够的醛基，用作与含胺分子或含酰肼分子高效偶联的靶标，包括其他蛋白、生物素试剂和荧光标记。由于糖蛋白含有其自身的伯胺基，可能发生氧化蛋白的聚合，除非在反应中加入足够摩尔过量的预期含胺靶分子。

相关产品
AminoLink™ 偶联树脂
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仅供科研使用。不可用于诊断程序。

数量2 x 1 g

产品线AminoLink™

类型还原剂

Unit SizeEach

内容与储存

在室温下干燥储存。在环境温度下运输

常见问题解答 (FAQ)

Does sodium azide interfere with AminoLink Support coupling?

No, the gel is stored in sodium azide with no loss in activity. Azide is not a primary amine and is rather unreactive.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between AminoLink Coupling Resin and NHS-Activated Agarose?

Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between AminoLink and AminoLink Plus supports?

AminoLink Plus Coupling Resin is activated at a higher level and has higher flow rates than the original AminoLink Coupling Resin, resulting in higher capacity and faster purification. AminoLink Supports can be used to immobilize any molecule with a primary amine.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do your AminoLink Coupling Resin and AminoLink Plus Coupling Resin work?

AminoLink and AminoLink Plus Supports are activated with aldehyde groups which will react with primary amines to form Schiff bases, which are reduced to stable, non-reversible secondary amines. Coupling efficiency often exceeds 85% with this support.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.