SulfoLink™ 蛋白质固定试剂盒,2 mL
SulfoLink™ 蛋白质固定试剂盒,2 mL
Thermo Scientific™

SulfoLink™ 蛋白质固定试剂盒,2 mL

Thermo Scientific SulfoLink 固定试剂盒(适用于蛋白质,2 mL)包含用于制备蛋白质样品进行有效固定的所有试剂,以及五个含有 2 mL SulfoLink了解更多信息
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货号数量
449955 个色谱柱试剂盒
货号 44995
价格(CNY)
9,398.00
Each
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数量:
5 个色谱柱试剂盒
请求批量或定制报价
价格(CNY)
9,398.00
Each
添加至购物车
Thermo Scientific SulfoLink 固定试剂盒(适用于蛋白质,2 mL)包含用于制备蛋白质样品进行有效固定的所有试剂,以及五个含有 2 mL SulfoLink 树脂并可以重力流或离心管形式用于进行高效耦合反应和多个周期亲和纯化的的色谱柱。

SulfoLink 固定试剂盒(适用于蛋白质,2 mL)的特点:

巯基 (-SH) 基团特异性—碘乙酸基团与巯基发生特异性反应,形成不可逆的硫醚键
快速—离心柱可提高方案速度;在 3.5 小时内制备和偶联蛋白质样品
灵活的偶联条件—可根据偶联反应期间的蛋白质或肽溶解度需求,使用 pH 为 7.5 至 9.0 的水性缓冲液、有机溶剂(例如 20% DMSO)或变性剂(盐酸·胍)
简单易懂的说明—简化的样品制备、固定化和亲和纯化方案
高容量—每 2mL SulfoLink 耦合树脂柱可固定 2 至 20mg 蛋白质

应用:
• 固定含末端半胱氨酸残基的各种肽以纯化由通过马来酰亚胺偶联制备的肽免疫原生成的各种抗体
•通过铰链区巯基,以定向方式固定各种抗体以确保抗原结合位点没有受到位阻,可进行抗原亲和纯化

在用含还原半胱氨酸残基的蛋白溶液孵育时,SulfoLink 树脂的碘乙酸基团与暴露的巯基 (-SH) 发生特异性高效反应,形成不可逆的共价硫醚键,将蛋白质永久固定到树脂上。产物是一种用于纯化各种抗体、抗原和其他目标分子的定制亲和树脂。

相关产品
SulfoLink™ 偶联树脂
SulfoLink™ 肽固定试剂盒,2 mL
SulfoLink™ 固定试剂盒试用装,2 mL
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和,琼脂糖树脂
产品规格离心柱
数量5 个色谱柱试剂盒
固定相卤乙酰
足够用于2-20 mg 抗体/柱
容量(公制)2 mL
产品线SulfoLink™
类型固定试剂盒
Unit SizeEach
内容与储存
足够用于:每柱固定 1 至 10 mg 抗体
• SulfoLink 柱,2 mL,5 个柱
• SulfoLink 制备缓冲液,7.5 mL
• SulfoLink 偶联缓冲液,500 mL
• 洗涤液,120 mL
• PBS 包(制备 500 mL),1 包
• 2-Mercaptoethylamine·HCl,5 x 6 mg
• L-Cysteine·HCl,100 mg
• Zeba 脱盐离心柱,5 mL,5 个离心柱
• 柱附件

在 4°C 下避光储存。

常见问题解答 (FAQ)

What is the binding capacity of SulfoLink Resin?

SulfoLink Resin can bind approximately 5 mg of reduced IgG per mL of support. For peptides, the capacity is approximately 1 mg of peptide per mL of gel. Capacity for other compounds will vary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When using the SulfoLink Coupling Resins and Kits, my peptide is not soluble in the coupling buffer. What can I do?

SulfoLink Immobilization is compatible with 3-4 M fresh urea or guanidine. Alternatively, dissolve the peptide in 100% DMSO. Add the peptide in DMSO to the coupling buffer so that the DMSO does not exceed 20% of the final solution.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Must I block the resin before each use when using the SulfoLink Coupling Resins and Kits?

No. The resin will no longer react with subsequent sulfhydryls after the ligand is attached and the remaining active sites are blocked.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How many purifications can I perform using the same SulfoLink Column?

The columns can be reused at least 10 times without loss of activity.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does SulfoLink Immobilization work?

SulfoLink Resin is crosslinked 6% beaded agarose that has been derivatized with a 12-atom spacer arm that ends in an iodoacetyl group. This spacer arm is helpful for peptides or large proteins that might otherwise be sterically hindered in binding their ligand. The iodoacetyl group reacts with free sulfhydryls at pH 7.5-8.5 to form covalent thioether bonds. The sulfhydryl must be present in the reduced form because disulfides will not react with the iodoacetyl group.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.