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Thermo Scientific™
SulfoLink™ 肽固定试剂盒，2 mL
Thermo Scientific SulfoLink Immobilization Kit for Peptides, 2 mL is a kit containing all the reagents for preparing the peptide了解更多信息
| 货号 | 数量 |
|---|---|
| 44999 | 5 个色谱柱试剂盒 |
货号 44999
价格（CNY)
8,370.00
Each
数量:
5 个色谱柱试剂盒
价格（CNY)
8,370.00
Each
Thermo Scientific SulfoLink Immobilization Kit for Peptides, 2 mL is a kit containing all the reagents for preparing the peptide sample for efficient immobilization and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.
Features of SulfoLink Immobilization Kit for Peptides, 2 mL:
• Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Fast—spin columns increase protocol speed; prepare and couple peptide samples in 2 hours
• Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity—Immobilize 1 to 2 mg of peptide per 2 mL column of SulfoLink Coupling Resin
Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
When incubated with a solution of peptide that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Related Products
SulfoLink™ Coupling Resin
SulfoLink™ Immobilization Kit for Proteins, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL
Features of SulfoLink Immobilization Kit for Peptides, 2 mL:
• Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Fast—spin columns increase protocol speed; prepare and couple peptide samples in 2 hours
• Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity—Immobilize 1 to 2 mg of peptide per 2 mL column of SulfoLink Coupling Resin
Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
When incubated with a solution of peptide that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Related Products
SulfoLink™ Coupling Resin
SulfoLink™ Immobilization Kit for Proteins, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和，琼脂糖树脂
产品规格离心柱
数量5 个色谱柱试剂盒
固定相卤乙酰
足够用于1-2 mg 巯基肽/柱
容量（公制）2 mL
产品线SulfoLink™
类型固定试剂盒
Unit SizeEach
内容与储存
足够用于：固定 0.1 至 1 mg 巯基肽/柱
•SulfoLink 柱，2 mL，5 个柱
•SulfoLink 偶联缓冲液，120 mL
•洗涤液，120 mL
•PBS 包（制成 500 mL），1 包
•Bond-Breaker TCEP 溶液，中性 pH 值，0.5 mL
•L-半胱氨酸·HCl，100 mg
•柱附件
避光储存在 4°C 下。
•SulfoLink 柱，2 mL，5 个柱
•SulfoLink 偶联缓冲液，120 mL
•洗涤液，120 mL
•PBS 包（制成 500 mL），1 包
•Bond-Breaker TCEP 溶液，中性 pH 值，0.5 mL
•L-半胱氨酸·HCl，100 mg
•柱附件
避光储存在 4°C 下。