We have modified the packaging to update labeling and improve the protection of our product, #62248. The improvements include thicker glass on the amber vial, and a two component closure that aims to protect from leakage. Additionally our internal verification testing and external customer validation data show that shipping these products at either ambient or freezing temperatures does not impact the functionality, performance, or specificity of staining.
DAPI 溶液 (1 mg/mL)
Thermo Scientific™

DAPI 溶液 (1 mg/mL)

Thermo Scientific Pierce DAPI 细胞核复染剂是用于细胞成像技术中对 DNA 含量和细胞核进行固定细胞荧光染色的高纯度形式的二脒基-2-苯基吲哚染料。可作为室温下稳定的即用型溶液提供:NucBlue Fixed Cell了解更多信息
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货号数量
622481 mL
货号 62248
价格(CNY)
1,990.00
飞享价
Ends: 31-Dec-2026
2,763.00
共减 773.00 (28%)
Each
数量:
1 mL
价格(CNY)
1,990.00
飞享价
Ends: 31-Dec-2026
2,763.00
共减 773.00 (28%)
Each
Thermo Scientific Pierce DAPI 细胞核复染剂是用于细胞成像技术中对 DNA 含量和细胞核进行固定细胞荧光染色的高纯度形式的二脒基-2-苯基吲哚染料。

可作为室温下稳定的即用型溶液提供:NucBlue Fixed Cell Ready Probes 试剂
请参见其他 Ready Probes 即用型成像试剂和附件›

DAPI(二脒基-2-苯基吲哚)是一种蓝色荧光探针,当其选择性地与双链 DNA 的小沟结合时会发出明亮的荧光,这种荧光约是非结合状态下荧光的20倍。其对 DNA 的选择性和高细胞可透过性可实现对细胞核的高效染色,而来自细胞质的背景极小。DAPI 是用于免疫荧光显微技术的经典细胞核复染剂,也是需要对 DNA 含量进行细胞定量的高内涵筛选方法的重要组成部分。

DAPI 细胞核复染剂的特点:

DAPI 染料—二脒基-2-苯基吲哚是一种 DNA 特异性蓝色荧光染色
便利—可选择粉末或易于使用的 DAPI 染料溶液 (1 mg/mL)
细胞成像—细胞渗透性染料可有效对固定细胞进行染色并对 DNA 含量进行定量
复染剂—适用于并行检测特异性靶标与荧光显微镜检查或高内涵筛选 (HCS) 荧光抗体

DAPI 荧光染料的特性:

其他名称:DAPI 染色剂、DAPI 染料、DNA 含量复染剂
化学名称:4,6’-二脒基-2-苯基吲哚二盐酸盐;4',6-二脒基-2-苯基吲哚;2-(4-脒苯基)-6-吲哚胍盐酸盐
分子式:C16H15N5·2HCl
分子量:350.25
激发波长:341±3 nm(与 dsDNA 结合时接近 360 nm)
发射波长:452±3 nm(与 dsDNA 结合时为 456 至 460 nm)
消光系数:347 nm 处 > 30,600/M cm,溶于甲醇中
CAS 号:28718-90-3
纯度:通过 HPLC 法测定,240 nm 处 > 95%(大多数批次 > 98%)
溶解度:> 1 mg/mL,溶于水中;化合物可溶于 DMF、水和各种非磷酸盐水性缓冲液
反应性基团:无;与双链 DNA 的小沟结合

作为荧光成像方法的复染剂,DAPI 可兼容荧光素罗丹明染料标记的抗体及其他探针以及 Thermo Scientific DyLight Fluor。DAPI 的光稳定性高于 Hoechst 染料,但是 Hoechst 33342 可用于活细胞成像,而 DAPI 仅适用于固定细胞。DAPI 以粉末固体和水溶液形式提供。

DAPI 染色剂的应用:

•检测溶液中的 DNA(参考4)
• 诊断细胞培养物的支原体感染(参考5)
• 在流式细胞分析中测量细胞核含量并对染色体进行分类(参考6)
• 评估细胞凋亡(参考7)
• 检测免疫荧光和原位杂交程序中的细胞核和有机物 DNA(参考2、8)
• 更换琼脂糖凝胶中用于 DNA 染色的溴化乙锭(参考5、9)
• 在利用红色荧光抗体检测特异性靶标的组织化学方法中对细胞核进行复染(参考8)
• 报告还指出 DAPI 可与多聚磷酸盐和其他多聚阴离子(参考10)、硫酸葡聚糖(参考11)和 SDS(参考12)结合。

相关产品
DAPI(4,’6-二脒基-2-苯基吲哚二盐酸盐)
仅供科研使用。不可用于诊断程序。
规格
颜色蓝色
最大浓度1 mg/mL
检测方法荧光
染料类型细胞不可透过性
发射460 nm
激发波长范围360 nm
适用于(应用)细胞成像
适用于(设备)荧光显微镜、高内涵仪器
产品线Pierce
数量1 mL
运输条件室温
标签类型Fluorescent Dye
产品类型DAPI
亚细胞定位细胞核/核仁
Unit SizeEach
内容与储存
在 2-8°C 下避光储存 DAPI 溶液 (1 mg/mL)。

常见问题解答 (FAQ)

我注意到,当我在click反应后用DAPI染色细胞并检测EdU的掺入时,相比于我的无click反应对照样品,DAPI信号非常低。是什么原因导致DAPI信号降低?

click反应中的铜离子使得DNA少量变性(虽然没有BrdU检测所需要的程度),这会影响到包括DAPI和Hoechst染色剂在内的DNA染料的结合亲和力。这种影响只会在传统的EdU试剂盒中出现,而Click-iT Plus EdU试剂盒由于采用的铜离子浓度低,不会出现这种现象。 

DAPI和Hoechst 染料非常相似,该如何选用?

DAPI是一种非常普通的蓝色核复染荧光染料,能对固定和通透的细胞和组织的细胞核进行非常明亮的标记。遗憾的是,人们普遍认为它是介于半通透性到非通透性之间的染色剂,对活细胞的染色效果也不一致。Hoechst 33342染料是细胞通透性染料,与DAPI染色有相似的染色结合机制和荧光颜色;它是活细胞成像的首选,且对固定细胞的标记效果和DAPI一样好。

Is DAPI a good live-cell nuclear label?

DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label inconsistenly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function.

For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, available in CFP, GFP and RFP. With these reagents, the cells are transduced overnight in a single labeling step and the next day the nuclei will fluoresce. The label may be retained for 3-5 days and should not affect cell function. Cytoplasmic cell tracking dyes such as the CellTracker dyes may also be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I notice that when I post-stain my cells with DAPI after performing the click reaction to detect EdU incorporation, my DAPI signal is lower compared to my no-click reaction control samples. What causes the reduction in DAPI signal?

The copper in the click reaction denatures DNA to a small extent (although not as much as is required for efficient BrdU detection), which can affect the binding affinity of DNA dyes including DAPI and Hoechst stain. This effect should only be apparent with the classic EdU kits and not the Click-iT Plus EdU kits, which use a lower copper concentration.

Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.