With what chemicals is the Slide-A-Lyzer MINI Dialysis Unit compatible?
The Slide-A-Lyzer MINI Dialysis Unit is compatible with alcohols, ketones, esters, oxides, and solvents containing nitrogen.
Alkalis: 1N sodium hydroxide or 1N ammonium hydroxide may cause pore swelling or shrinkage; not recommended to be used with 1N potassium hydroxide
Acids: Will survive weak acids (100% acetic or formic acids), or ~25% phosphoric or sulfuric acids; it should not be used with >25% strong acids, although a weak solution of a strong acid may work
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
What percent of my sample should I expect to lose during dialysis when using the Slide-A-Lyzer MINI Dialysis Unit?
In general, polypropylene and regenerated cellulose have very low nonspecific binding properties. The percent recovery of a sample will depend on the unique properties of the sample and on the initial concentration of the sample.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
What materials are used to manufacture the Slide-A-Lyzer MINI Dialysis Unit?
The membrane is regenerated cellulose and the plastic is a polypropylene copolymer. Both materials have very low protein and nucleic acid binding properties.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
How long does it take to dialyze with the Slide-A-Lyzer MINI Dialysis Unit?
The Slide-A-Lyzer MINI Dialysis Unit can convert 100 µL of pH 2.8 buffer to pH 9.4 dialyzing against 1 liter bicarbonate buffer, pH 9.4 in less than 10 minutes.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
How do I use the Slide-A-Lyzer MINI Dialysis Unit?
- Wearing gloves, apply sample (0.01-0.1 mL) using a pipette.
- OPTIONAL: Soak the Slide-A-Lyzer MINI Dialysis Unit to remove trace contaminants.
- Cap the Slide-A-Lyzer MINI Dialysis Unit (to minimize loss) and snap it into the float (sold separately).
- Insert the float into the beaker containing the dialysate.
- Recover sample.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.