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View additional product information for Slide-A-Lyzer™ MINI Dialysis Devices, 10K MWCO - FAQs (69576, 88404, 69570, 88401, 69574, 69572)
36 product FAQs found
The Slide-A-Lyzer MINI Dialysis Unit is compatible with alcohols, ketones, esters, oxides, and solvents containing nitrogen.
Alkalis: 1N sodium hydroxide or 1N ammonium hydroxide may cause pore swelling or shrinkage; not recommended to be used with 1N potassium hydroxide
Acids: Will survive weak acids (100% acetic or formic acids), or ~25% phosphoric or sulfuric acids; it should not be used with >25% strong acids, although a weak solution of a strong acid may work
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
In general, polypropylene and regenerated cellulose have very low nonspecific binding properties. The percent recovery of a sample will depend on the unique properties of the sample and on the initial concentration of the sample.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The membrane is regenerated cellulose and the plastic is a polypropylene copolymer. Both materials have very low protein and nucleic acid binding properties.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The Slide-A-Lyzer MINI Dialysis Unit can convert 100 µL of pH 2.8 buffer to pH 9.4 dialyzing against 1 liter bicarbonate buffer, pH 9.4 in less than 10 minutes.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
- Wearing gloves, apply sample (0.01-0.1 mL) using a pipette.
- OPTIONAL: Soak the Slide-A-Lyzer MINI Dialysis Unit to remove trace contaminants.
- Cap the Slide-A-Lyzer MINI Dialysis Unit (to minimize loss) and snap it into the float (sold separately).
- Insert the float into the beaker containing the dialysate.
- Recover sample.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
To remove glycerol, dialyze for 15 minutes against 1 liter of deionized water. Glycerol content is less than 3% in the 3K unit, approximately 15% in the 7K unit and 23% in the 10K unit. To remove metals, dialyze for 15 minutes against 1 liter of 1 mM EDTA. Metals present in the 3K, 7K and 10K unit include 2 ppb iron, 5 ppb magnesium, 1.5 ppb nickel, 0.2 ppb zinc, 0.2 ppb copper, 0.5 ppb chromium and 0.3 ppb cadmium. To remove sulfur, dialyze for 15 minutes against 1 liter of deionized water. Sulfur content should be minimal, less than 0.1%. Thermo Scientific quality control checks for sulfur on the membrane prior to the unit's assembly.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Always wear gloves when touching a Slide-A-Lyzer MINI Dialysis Unit If desired, soak the Slide-A-Lyzer MINI Dialysis Unit to remove contaminants For best recovery, tilt the tube and collect the sample in the bottom corner of the device.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The Slide-A-Lyzer MINI Dialysis Unit is ideal for:
- Salt reduction Buffer exchange (<10 min for pH change 2.8 to 9.5)
- Removal of small molecular weight compounds
- Micro-conjugation (reagent addition or sample evaluation anytime)
- Equilibrium dialysis (microtube, paraffin to adjust depth)
- Concentration (in a microtube concentrates @ 35-45 µl/hr; use at least a 3:1 v/v ratio of concentrator to sample.)
- Sample preparation for instrumentation analysis (HPLC, CE, MS, GC, electrophoresis, etc.)
- Sample preparation for proteins, peptides, oligonucleotides, DNA, RNA (~330 MW/base ~660 MW/bp)
- The “scavenger” technique in which a resin, membrane, polymer or protein is placed in a microtube with buffer in order to select a compound to remove from a sample in a Slide-A-Lyzer MINI Dialysis Unit.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
It is possible to add reagents to the Slide-A-Lyzer Dialysis Cassette at different times using different portholes, allow a reaction to occur, and then dialyze in the same Slide-A-Lyzer Dialysis Cassette, reducing sample loss through reduced sample handling.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The dialysis membranes are made of regenerated cellulose, which may cause some molecules to stick nonspecifically resulting in sample loss. The percent of total protein lost is partially dependent on the protein concentration. Protein loss caused by nonspecific binding to the membrane is negligible for concentrated samples (greater than 0.5 mg/mL) but may be significant with dilute protein samples (<0.1 mg/mL). Adding a “carrier” protein such as BSA to dilute protein sample before dialysis will prevent this loss.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
Water moves quickly and easily across the dialysis membrane. When dialyzing a high solute concentration against a dilute dialysis buffer, there will be a net movement of water (and possibly salts) into the dialysis unit through the membrane. Glycerol and some sugars are especially hygroscopic, and as rapidly as they diffuse across the membrane to reach equilibrium, they also significantly affect the osmosis of water across the membrane and so may cause a change in volume of the sample. Take care when dialyzing with large differences in glycerol or sugar concentration between sample and dialysis membrane. Prevent this movement of water and consequent change in sample volume by dialyzing in a “stepwise” fashion, minimizing the difference in water concentration between sample and dialysis buffer at each stage in the dialysis process (see more in Dialysis: An Overview - https://tools.thermofisher.com/content/sfs/brochures/TR0020-Dialysis-overview.pdf).
The smallest sizes of the Slide-A-Lyzer Mini Dialysis Units (10-100 µL) are inserted into a float. If the top of the sample is below the top of the buffer, then hydrostatic pressure of the buffer will force buffer into the unit, increasing the volume and diluting the sample. Making sure that the dialysis membrane is in contact with the buffer and the top of the sample is at or above the level of the buffer will prevent this.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
We do not recommend re-use.
Yes, but we‘d suggest pre-treatment of the Slide-A-Lyzer device with EDTA or other DNase/RNase pre-treatment.
Yes, the MINI Slide-A-Lyzer device can be used as a concentrating device as described in the following article: (https://assets.thermofisher.com/TFS-Assets/LSG/brochures/TR0078-SAL-Conc-Solution.pdf). The average concentration rate of 35 µL/hour was observed in house when the sample was in PBS. We recommend using at least a 3:1 v/v ratio of concentrator to sample. In general, increased salt concentration in the sample will reduce the rate of sample concentration.
The MINI Slide-A-Lyzer devices are not certified sterile but they are manufactured in a clean environment. HEPA filters keep out contaminants. From molding to packaging, the MINI components have never been touched with ungloved (powder-free latex or nitrile) hands.
In general, colder solutions take longer to dialyze. Dialysis completion will depend on the composition and volume of sample and dialysate. Simple pH exchange proceeds very rapidly; less than 10 minutes for 100 µL to change from pH 2.8 to pH 9.4. Dialysis of a 1 M salt solution against water proceeds very rapidly (less than 10 minutes for 10 µL). Dialysis of 100 µL of small compounds, 500-1,500 daltons, against a saline solution will be approximately 50% complete in 2-4 hours or 99% or higher complete after overnight when dialyzing against approximately 1 liter of buffer. Dialysis will proceed faster with more frequent buffer changes.
Yes, the 0.1 mL devices can be placed into a foam float during dialysis, while the 0.5 mL and 2 mL sizes are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively.
The MINI Dialysis Units are made of regenerated cellulose membrane, and the cup is made of polypropylene. The Units require 2 minutes‘ hydration time before use.
The Slide-A-Lyzer Dialysis Cassette is the same regenerated cellulose as other dialysis tubing so you can expect about the same amount of protein loss as with tubing. As a guide, a 1 mg/mL solution will have a recovery rate of greater than 95%; at 100 mg/mL the recovery drops to 75-80%; and in solutions as dilute 10 µg/mL users may observe only a 50% (or less) recovery.
Dialyze for the amount of time sufficient to remove low molecular weight compounds for the specific downstream application. A typical dialysis procedure is as follows:
1) Dialyze for 2 hrs at room temperature or 4 degrees C.
2) Change the dialysis buffer and dialyze for another 2 hrs.
3) Change the dialysis buffer and dialyze overnight at 4 degrees C.
Use the dialysis buffer at 200-500 times the volume of the sample. For example, if the starting sample volume is 1 mL, then use a dialysis buffer amount that is equal to 200 mL-500 mL for each dialysis buffer change.
The buoy plays two roles: it suspends the Slide-A-Lyzer Dialysis Cassette above the stirring bar during dialysis; it provides a stand to set the Slide-A-Lyzer Dialysis Cassette in on the bench-top
No, any 18 gauge needle at least one inch long, and syringes of sufficient volume will work.
The Slide-A-Lyzer Dialysis Cassette can be held at 56 degrees C for 2 hours. At temperatures and times above these, the ABS plastic gets soft and can deform. We recommend maintaining the pH range of 5-9.
No. The Slide-A-Lyzer Dialysis Cassette membrane is of a grade used for human kidney dialysis and requires no pretreatment. However, when using small volumes we do recommend pre-wetting the units in deionized water for 30 seconds. Trace metals and 12% glycerol are present in all Slide-A-Lyzer Dialysis Cassettes. During the dialysis process these will dialyze away to virtually no concentration remaining in the cassette.
The plastic casing is made of ABS plastic and the gasket is made from a non-leaching silicone-type material. The membrane is made from regenerated cellulose. We have successfully used 10% solutions of DMF, DMSO, acetonitrile, methanol, hexane, heptane, 100% acetic acid, and 70% ethanol with the device. The units are compatible with 75% acetonitrile and 1% TFA in the sample, however we recommend not dialyzing into a buffer of acetonitrile concentration greater than 10%.
There are 3.5 K, 7K, 10K, and 20K MWCOs available in 0.1-0.5 mL, 0.5-3 mL, 3-12 mL, and 12-30 mL. These units, especially at the smaller volumes, provide for greater sample recovery and shorter dialysis times than traditional tubing due to their greater surface area-to-volume ratio. For samples smaller than 100 µL, use the Slide-A-Lyzer MINI Dialysis Units and for larger volumes, refer to our SnakeSkin Dialysis Tubing.
No, the Slide-A-Lyzer Dialysis Cassette is designed as a single-use, disposable device. Once hydrated, the membrane immediately begins to dry after the sample is removed: and the whole membrane will be dry within a few minutes, changing the molecular weight cut-off (MWCO).
There is an unseen internal gasket which reseals as the needle is withdrawn. The most important items to remember are:
Fill with air first to check that system is airtight
Puncture each porthole only once
Do not allow the membrane to come in contact with the sharp bevel of the needle
Cassettes have easy handling and secure sample delivery, while traditional tubing is slippery when wet and can be difficult to handle, increasing risk of sample loss during sample addition/removal. Cassettes provide sample protection with a welded membrane and leak-proof cap, helping to minimize the risk of sample loss, whereas with traditional tubing, samples can leak out if the clamp is loose/falls off. Lastly, cassettes are fast and efficient with high surface/volume ratio allowing for more rapid dialysis than conventional tubing.
Please see the protocol summary below:
Insert syringe needle through the gasket via one of the corner ports. Inject the sample, withdraw the excess air and remove the syringe.
Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes).
Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, then withdraw the dialyzed sample.
The kit includes float buoys and syringes, and are only available for first-generation products. Please note that floats and syringes can also be purchased separately.
We do not test for or claim sterility.
Yes, we offer gamma-irradiated array Slide-A-Lyzer cassettes for both first-generation products (Cat. No. 66454, 66455, 66453, 66456) and second-generation products (Cat. No. 88250, 882551, 882552, 882553, 882554).
The first generation cassettes can require a syringe to insert the sample, and may require a float buoy. They can hold 0.5-30 mL solution. The second-generation cassettes can be loaded with a syringe or pipet, are self-floating, and can hold 0.5-70 mL.
Please view our selection table to choose the right dialysis device for your experiment - https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/dialysis-products.html.
Dialysis is the separation of small and large molecules in a solution by selective diffusion through a semi-permeable membrane. It is generally used for larger sample volumes, and can take hours to overnight for complete dialysis.