Targeted Protein Degradation through Recruitment of the CUL4 Complex Adaptor Protein DDB1
AuthorsMeyers M, Cismoski S, Panidapu A, Chie-Leon B, Nomura DK.
JournalACS Chem Biol
PubMed ID38192078
Targeted protein degradation has arisen as a powerful therapeutic modality for eliminating proteins. Thus far, most heterobifunctional proteolysis targeting chimeras (PROTACs) have utilized recruiters against substrate receptors of Cullin RING E3 ubiquitin ligases, such as cereblon and VHL. However, previous studies have surprisingly uncovered molecular glue degraders that exploit a ... More
Generating high quality libraries for DIA MS with empirically corrected peptide predictions.
AuthorsSearle BC,Swearingen KE,Barnes CA,Schmidt T,Gessulat S,Küster B,Wilhelm M
JournalNature communications
PubMed ID32214105
Data-independent acquisition approaches typically rely on experiment-specific spectrum libraries, requiring offline fractionation and tens to hundreds of injections. We demonstrate a library generation workflow that leverages fragmentation and retention time prediction to build libraries containing every peptide in a proteome, and then refines those libraries with empirical data. Our method ... More
Proteomics of prostate cancer serum and plasma using low and high throughput approaches
Despite progress, MS-based proteomics in biofluids, especially blood, faces challenges such as dynamic range and throughput limitations in biomarker and disease studies. In this work, we used cutting-edge proteomics technologies to construct label-based and label-free workflows, capable of quantifying approximately 2,000 proteins in biofluids. With 70µL of blood and a ... More