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查看更多产品信息 Tween™ 20 Surfact-Amps™ Detergent Solution - FAQs (28320, 85115, 28321, 85114, 85113)
5 个常见问题解答
Tween-20 Surfact-Amps Detergent Solution is a highly-purified Tween 20 detergent stabilized as a 10% solution and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring its stability and eliminating the accumulation of peroxides and degradation products.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The detergent is screened for:
- Visualization: clear, light yellow liquid, free of particulates
- Concentration: 10.0 +/- 1.0%
- Oxidant concentration: less than or equal to 1.0 µeq/mL
- Carbonyl concentration: less than or equal to 1.0 µeq/mL
- suspended solids: residue present must not exceed Residue Reference
See the COA for further details.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Thermo Scientific Pierce Surfact-Amps detergents are highly purified, precisely diluted (10%) formulations that are ideal for applications or assays that are sensitive to contaminants that are present in unpurified detergents. We test every batch to insure that our detergents contain less than 1.0 µeq/mL peroxides and carbonyls and package them under nitrogen, to prevent oxidization during storage.
Superior quality - lower measurable contaminant levels than other leading vendors
Accurate -precise 10% detergent solution in ultrapure water
Easy-to-use - solution is simple to dispense and dilute for use
Exceptionally pure - less than 1.0 µeq/mL peroxides and carbonyls
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head”. The ionic character of the polar head group forms the basis for broad classification of detergents as ionic, nonionic, or zwitterionic.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.