Evaluation of the tert-butyl group as a probe for NMR studies of macromolecular complexes
AuthorsVoleti R, Bali S, Guerrero J, Smothers J, Springhower C, Acosta GA, Brewer KD, Albericio F, Rizo J.
JournalJ Biomol NMR
PubMed ID34505210
The development of methyl transverse relaxation optimized spectroscopy has greatly facilitated the study of macromolecular assemblies by solution NMR spectroscopy. However, limited sample solubility and stability has hindered application of this technique to ongoing studies of complexes formed on membranes by the neuronal SNAREs that mediate neurotransmitter release and synaptotagmin-1, ... More
BPP43_05035 is a Brachyspira pilosicoli cell surface adhesin that weakens the integrity of the epithelial barrier during infection.
AuthorsRajan A,Gallego P,Dolan B,Patel P,Dwibedi C,Luis AS,Trillo-Muyo S,Arike L,van der Post S,Simrén M,Pelaseyed T
JournalGut microbes
PubMed ID39349383
The anaerobic spirochete Brachyspira causes intestinal spirochetosis, characterized by the intimate attachment of bacterial cells to the colonic mucosa, potentially leading to symptoms such as diarrhea, abdominal pain, and weight loss. Despite the clinical significance of Brachyspira infections, the mechanism of the interaction between Brachyspira and the colon epithelium is ... More
Effects of GDF6 on active protein synthesis by cells of degenerated intervertebral disc.
AuthorsTam V,Chopra N,Sima S,Chen P,Sharma R,Chan D,Diwan A
JournalEuropean spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
PubMed ID39920317
INTRODUCTION: Intervertebral disc degeneration (IVD) is a leading cause of low back pain, a prevalent musculoskeletal condition. IVD degeneration is characterized by the degradation of nucleus pulposus (NP), annulus fibrosus (AF), and cartilage endplates (EP). Growth Differentiation Factor 6 (GDF6), part of the bone morphogenetic protein family, has demonstrated potential ... More