Pierce™ IP Lysis Buffer, 250 mL - FAQs

View additional product information for Pierce™ IP Lysis Buffer - FAQs (87788, 87787)

12 product FAQs found

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Can you provide the shelf-life for the Pierce IP Lysis Buffer?

The Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can you provide the shelf life for Pierce IP Lysis Buffer?

Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I store Pierce IP Lysis Buffer (Cat. No. 87787) at room temperature?

Pierce IP Lysis Buffer ships at ambient temperature and can be stored at room temperature. However, we recommend 4 degrees C for long term storage in order to prevent possible bacterial growth.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Which protein assays are compatible with the Thermo Scientific IP Lysis Buffer?

The Thermo Scientific IP Lysis Buffer is compatible with Thermo Scientific BCA Protein Assay (Cat. No. 23225) and Thermo Scientific 660 nm Protein Assay (Cat. No. 22660). It is not compatible with Thermo Scientific Coomassie Plus (Bradford) Assay (Cat. No. 23236) unless diluted 1:1 in TBS, PBS, or water.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the basic protocol to use with the Thermo Scientific IP Lysis Buffer?

For suspension cells, use 500 µl of lysis buffer per 50 mg of wet cell pellet (10:1 v/w). Incubate lysate on ice for 5 minutes with periodic mixing. Remove cell debris by centrifugation at ~13,000 × g for 10 minutes at 4 degrees C. Transfer supernatant to a new tube.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Does the Thermo Scientific IP Lysis Buffer contain protease inhibitors?

The Thermo Scientific IP Lysis Buffer does not contain protease or phosphatase inhibitors. We recommend inhibitors, such as Thermo Scientific Halt Protease Inhibitor Cocktail (Cat. No. 87786) or Phosphatase Inhibitor Cocktail (Cat. No. 78420) to be added to the buffer just before use.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why is the Thermo Scientific IP Lysis Buffer the recommended lysis buffer for preparing samples for co-immunoprecipitation (Co-IP)?

The gentle formulation of the buffer helps maintain protein complexes for co-immunoprecipitation.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When should I use Thermo Scientific IP Lysis Buffer?

The Thermo Scientific IP Lysis Buffer is recommended for scientists working with mammalian cells in order to extract cellular proteins for Immunoprecipitation and Western Blot analysis. It is effective for lysing cultured mammalian cells from both plated cells and cells pelleted from suspension cultures, and is especially formulated for pull-down and immunoprecipitation assays, and as a wash buffer for affinity resins. This lysis buffer is also compatible with protein purification, immunoassays and ELISA.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the composition of the Thermo Scientific IP Lysis Buffer (Cat. No. 87787)?

The Thermo Scientific IP Lysis Buffer contains 25 mM Tris.HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol. This formulation is optimized for compatibility with immunoprecipitation and pull-down assays and does not contain SDS.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is the Thermo Scientific IP Lysis Buffer similar to RIPA buffer?

The Thermo Scientific IP Lysis Buffer (Cat. No. 87787) is a mammalian whole cell lysis buffer based on a modified RIPA buffer formulation without SDS. This moderate strength lysis buffer is able to effectively solubilize cellular proteins but does not liberate genomic DNA or disrupt protein complexes like RIPA buffer.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What lysis buffers do you offer for mammalian cells?

Please see the various lysis buffers we offer (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/cell-lysis-total-protein-extraction.html) for total protein extraction from mammalian cells.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.