What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?
The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).
Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.
Can you provide the shelf-life for the Pierce IP Lysis Buffer?
The Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Can you provide the shelf life for Pierce IP Lysis Buffer?
Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Can I store Pierce IP Lysis Buffer (Cat. No. 87787) at room temperature?
Pierce IP Lysis Buffer ships at ambient temperature and can be stored at room temperature. However, we recommend 4 degrees C for long term storage in order to prevent possible bacterial growth.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Which protein assays are compatible with the Thermo Scientific IP Lysis Buffer?
The Thermo Scientific IP Lysis Buffer is compatible with Thermo Scientific BCA Protein Assay (Cat. No. 23225) and Thermo Scientific 660 nm Protein Assay (Cat. No. 22660). It is not compatible with Thermo Scientific Coomassie Plus (Bradford) Assay (Cat. No. 23236) unless diluted 1:1 in TBS, PBS, or water.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.