HisPur™ Ni-NTA 树脂
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HisPur™ Ni-NTA 树脂
Thermo Scientific™

HisPur™ Ni-NTA 树脂

Thermo Scientific HisPur Ni-NTA 树脂是高容量、高性能镍-IMAC 树脂,用于常规亲和纯化 His 标签融合蛋白。HisPur了解更多信息
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货号数量
8822110 mL
88222100 mL
88223500 mL
货号 88221
价格(CNY)
1,205.00
飞享价
Ends: 31-Dec-2025
1,896.00
共减 691.00 (36%)
Each
添加至购物车
数量:
10 mL
请求批量或定制报价
价格(CNY)
1,205.00
飞享价
Ends: 31-Dec-2025
1,896.00
共减 691.00 (36%)
Each
添加至购物车
Thermo Scientific HisPur Ni-NTA 树脂是高容量、高性能镍-IMAC 树脂,用于常规亲和纯化 His 标签融合蛋白。

HisPur Ni-NTA 树脂的特点:

高容量—每毫升树脂可结合高达 60 mg 的 6xHis-标签蛋白
通用—可在天然或变性条件下纯化蛋白
兼容—与 Thermo Scientific 细胞裂解试剂和各种缓冲液添加剂配合使用
高性价比—一批树脂可重复使用至少五次
易于使用—试剂盒规格提供预配制缓冲液
灵活—具有多种形式,包括散装树脂、离心柱、色谱纯化柱和 96 孔过滤板

特殊制备的支持物含有微珠状琼脂糖,使用次氮基三乙酸 (NTA) 螯合部分进行衍生化,并带有螯合二价镍离子 (Ni2+)。固定化金属亲和色谱 (IMAC) 树脂拥有用于重组 His 标签蛋白纯化的绝佳结合容量和性能。

重组蛋白的表达和纯化对于蛋白调节、结构和功能研究至关重要。大部分的重组蛋白都以短亲和标记的融合表达,最流行的是聚组氨酸(6xHis)标记。用于纯化重组 His 标签蛋白的方法是固定化金属亲和色谱 (IMAC),由带镍或钴离子的螯合树脂组成,镍或钴离子与组氨酸侧链配位。

HisPur Ni-NTA 树脂能从细菌裂解物(如使用 Thermo Scientific B-PER 细菌蛋白提取试剂获得的裂解物)中有效纯化高水平过表达 His 标签融合蛋白。该树脂在各种容量的批连结和离心柱程序中表现很好。性能等同于或超过来自其他供应商的各种常用镍-NTA树脂。HisPur镍-NTA树脂是一种高品质、稳定而有弹性的亲和载体。各种试验证实在至少五次重复使用后,其性能不会降低。这些数据显示在正常使用中,该树脂能高度抵抗结构降解或镍离子浸出。

HisPur Ni-NTA 树脂和 HisPur 钴树脂是 IMAC 的替代形式,前者使用镍,后者使用钴作为负责 His-标签结合的螯合金属离子。由于其在螯合上的四个能高蛋白连结和低金属离子滤去的金属连结点,镍-NTA树脂已经成为6xHis标记蛋白纯化最常用的IMAC树脂选择。钴连结不太强健,因此更有区分性,使其在 His 标签蛋白纯化中有更高的纯度但常常更低的得率。

可用形式:
树脂浆—交联 6% 微珠状琼脂糖;10 mL、100 mL、500 mL 瓶
离心柱— 0.2 mL(微量离心机)、1 mL 和 3 mL 离心柱
纯化试剂盒—所有三种柱规格的全套试剂盒
色谱纯化柱—了解更多信息并参见 HisPur 色谱纯化柱数据
96孔离心板

相关产品
HisPur™ Ni-NTA 离心柱,0.2 mL
HisPur™ Ni-NTA 离心柱,1 mL
HisPur™ Ni-NTA 离心柱,3 mL
HisPur™ Ni-NTA 色谱纯化柱,1 mL
HisPur™ Ni-NTA Superflow 琼脂糖
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和,琼脂糖树脂
描述HisPur 镍-NTA树脂
产品规格瓶装
数量10 mL
固定相二价镍、金属螯合物
靶标His 标签蛋白
容量(公制)结合多达 60 mg 6xHis 标签蛋白/mL 树脂
形式悬浮液
产品线HisPur™
类型树脂
Unit SizeEach
内容与储存
在4°C条件下储存

常见问题解答 (FAQ)

Are there other sequences that can bind to nickel more tightly than 6xHis-tagged proteins and how can they be eluted.

Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can the HisPur Ni-NTA Spin plates (Cat. No. 88230) be regenerated and reused, or are they for single-use only?

The resin can be re-used at least five times; this is also for the resin in the plates. Regeneration is possible using this procedure (https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2FMAN0011700_HisPur_NiNTA_Resin_UG.pdf) Since the resin bed volume in each well is 50 µL, you can use 250 µL twice to give a total of 10 resin-bed volume.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do I perform Ni-NTA bead regeneration with the HisPur Ni-NTA Resin (Cat. No. 88221)?

Please refer to page 2 (buffer preparation) and page 3 (procedure) of the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011700_HisPur_NiNTA_Resin_UG.pdf) for information regarding bead regeneration.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.