Pierce™ 蛋白 A 磁珠
Pierce™ 蛋白 A 磁珠
Thermo Scientific™

Pierce™ 蛋白 A 磁珠

Thermo Scientific Pierce 蛋白 A 磁珠为高容量、高通量亲和微粒,用于抗体纯化和免疫沉淀方法(采用手动或自动磁性分离器)。蛋白了解更多信息
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货号数量
888451 mL
888465 mL
货号 88845
价格(CNY)
2,641.00
Each
添加至购物车
数量:
1 mL
请求批量或定制报价
价格(CNY)
2,641.00
Each
添加至购物车
Thermo Scientific Pierce 蛋白 A 磁珠为高容量、高通量亲和微粒,用于抗体纯化和免疫沉淀方法(采用手动或自动磁性分离器)。

蛋白 A 磁珠的特点:

高效– IP 靶抗原得率与其他供应商的磁珠相当或高于后者
低非特异性结合– 稳定、预封闭的磁珠可提供高纯度产物(例如,IP 中与抗体一起洗脱的抗原中无来自复杂细胞裂解物的污染性蛋白)
一致– 磁珠可消除树脂损耗,与仅使用离心的传统 IP 方法相比,分离效率更高
通用– 微珠可与手动和自动工作流程(例如,Thermo Scientific KingFisher 仪器)兼容

重组蛋白 A 共价结合于封闭磁珠表面,可结合到来自多种不同物种的抗体上,从而可对粗提取物中的抗体进行纯化。用包被蛋白 A 的磁珠进行的免疫沉淀反应可获得高产量靶抗原和极低的背景使用我们的交联剂,您可以将抗体固定到磁珠上,防止 IP 或 Co-IP 实验中的 IgG 污染。Pierce 蛋白 A 磁珠可与磁力架配合用于手工操作,亦可用于自动化平台,如 Thermo Scientific KingFisher 仪器,以用于高通量工作流程。

应用:
• IP 和 Co-IP 实验
• 抗体纯化

Thermo Scientific Pierce 蛋白 A 磁珠用于血清、细胞培养物上清液或腹水中的抗体纯化以及对细胞或组织提取物中的抗原进行的 IP/Co-IP。蛋白 A 可结合多种不同物种来源的抗体,这些物种包括小鼠、人、兔、猪、犬和猫。Pierce 蛋白 A 磁珠的实验方案已经优化,以提供高回收率和高纯度的分离抗体或抗原。抗体或抗原/抗体复合物 (IP) 首先被捕获于磁珠上。洗涤微珠,随后以低 pH 值洗脱缓冲液洗脱靶标。免疫沉淀的性能与其他供应商提供的蛋白 A 磁珠相当或优于后者。
仅供科研使用。不可用于诊断程序。

Thermo Scientific™ Pierce 蛋白 A 磁珠用于血清、细胞培养物上清液或腹水中的抗体纯化以及对细胞或组织提取物中的抗原进行的 IP/Co–IP。蛋白 A 可结合多种不同物种来源的抗体,这些物种包括小鼠、人、兔、猪、犬和猫。Pierce 蛋白 A 磁珠的实验方案已经优化,以提供高回收率和高纯度的分离抗体或抗原。抗体或抗原/抗体复合物 (IP) 首先被捕获于磁珠上。洗涤微珠,随后以低 pH 值洗脱缓冲液洗脱靶标。免疫沉淀的性能与其他供应商提供的蛋白 A 磁珠相当或优于后者。

规格
最大浓度Slurry: 10mg/mL, 1% solids
配基类型蛋白 A
蛋白质形式重组
数量1 mL
靶标抗体
容量(公制)1 mL
形式Liquid
粒径1 μm
产品线Pierce™
类型磁珠
Unit SizeEach
内容与储存
接收后,在 4°C 下储存。产品在冰袋上运输。

常见问题解答 (FAQ)

We would like to crosslink our antibodies to Protein A magnetic beads rather than switch to the Protein A/G beads. Will the protocol and reagents described in the protocol for the Protein A/G beads work for the Protein A beads?

Protein A as well as Protein A/G bind to antibodies via the Fc portion. You can use DSS crosslinker to crosslink the antibody to Protein A as we described it for Protein A/G in our kit (Cat No. 88805). Please note that with this chemical crosslinking, there is the potential to lose antigen binding sites, and thus, the IP yield may be a less than when performing the IP without crosslinking.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the differences between Protein A, Protein G, Protein A/G, and Protein L Ig-binding proteins?

Protein A, Protein G, and Protein A/G bind almost exclusively to the IgG class of antibodies, but their binding properties differ among species and subclasses of IgG. Protein L binds in the variable fragment of some kappa light chains and can react with any immunoglobulin, not just IgG, as long as the correct kappa light chains are present. Protein L does not bind lambda light chains and certain kappa chains of different species.

-Protein A is generally preferred for rabbit, pig, dog, and cat IgG.
-Protein G has better binding capacity for a broader range of mouse and human IgG subclasses (e.g., IgG1 vs. IgG2)
-Protein A/G is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein G. Therefore, Protein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human, and other mammalian samples.
-Protein L binds to certain immunoglobulin kappa light chains. Because kappa light chains occur in members of all classes of immunoglobulin (i.e., IgG, IgM, IgA, IgE and IgD), Protein L can purify these different classes of antibody. However, only those antibodies within each class that possess the appropriate kappa light chains will bind. Generally, empirical testing is required to determine if Protein L is effective for purifying a particular antibody. It binds only Vk1 in mouse and VkI, VkIII and VkIV in human.
Read more about the general characteristics of Ig-binding proteins (https://www.thermofisher.com/us/en/home/life-science/antibodies/antibody-purification-kits-reagents.html) and (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0034-Ab-binding-proteins.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.