Lectin affinity as an approach to the proteomic analysis of membrane glycoproteins.
AuthorsGhosh D, Krokhin O, Antonovici M, Ens W, Standing KG, Beavis RC, Wilkins JA
JournalJ Proteome Res
PubMed ID15359739
The aim was to determine the proportion of membrane glycoproteins captured using concanavalin A or wheat germ agglutinin lectin affinity chromatography. Digests of the isolated proteins were separated by reversed-phase liquid chromatography and analyzed by matrix-assisted laser desorption tandem mass spectrometry. The two lectins identified different groups of proteins with ... More
GlycoSuiteDB: a new curated relational database of glycoprotein glycan structures and their biological sources.
AuthorsCooper CA, Harrison MJ, Wilkins MR, Packer NH
JournalNucleic Acids Res
PubMed ID11125129
GlycoSuiteDB is a relational database that curates information from the scientific literature on glyco-protein derived glycan structures, their biological sources, the references in which the glycan was described and the methods used to determine the glycan structure. To date, the database includes most published O:-linked oligosaccharides from the last 50 ... More
Development of serum glycoproteomic profiling technique; simultaneous identification of glycosylation sites and site-specific quantification of glycan structure changes.
AuthorsUeda K, Takami S, Saichi N, Daigo Y, Ishikawa N, Kohno N, Katsumata M, Yamane A, Ota M, Sato TA, Nakamura Y, Nakagawa H
JournalMol Cell Proteomics
PubMed ID20811073
Characterization and interpretation of disease-associated alterations of protein glycosylation are the central aims of the emerging glycoproteomics projects, which are expected to lead to more sensitive and specific diagnosis and improve therapeutic outcomes for various diseases. Here we report a new approach to identify carbohydrate-targeting serum biomarkers, termed isotopic glycosidase ... More