培养细胞线粒体分离试剂盒

The Mitochondria Isolation Kit for Cultured Cells is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The typical yield is approximately 150 µg protein from 20 million starting cells after mitochondrial isolation and lysis, based on HeLa cells. This varies with cell type.

Yes, the kit offers an optimized Dounce homogenization procedure, which results in two-fold greater mitochondri recovery compared to reagent-based method.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

To obtain a more purified fraction of heavy mitochondria, centrifuge the post-nuclear supernatant at 3000g rather than 12,000g. Western blot analysis of purified versus crude mitochondrial fractions prepared with this kit result in a > 50% reduction in cathepsin S (lysosomal protein) and PMP70 (peroxisomal protein) in the purified fraction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

We offer the following kits for cell fractionation:

Mem-PER Plus Membrane Protein Extraction Kit, Cat. No. 89842
NE-PER Nuclear and Cytoplasmic Extraction Reagents, Cat. No. 78835
Subcellular Protein Fractionation Kit for Cultured Cells, Cat. No. 78840
Subcellular Protein Fractionation Kit for Tissues, Cat. No. 87790
Mitochondrial Isolation Kit for Cultured Cells, Cat. No. 89874
Mitochondrial Isolation kit for Tissue, Cat. No. 89801
Lysosome Enrichment Kit for Tissues and Cultured Cells, Cat. No. 89839

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.