Mitochondria Isolation Kit for Cultured Cells - FAQs

View additional product information for Mitochondria Isolation Kit for Cultured Cells - FAQs (89874)

6 product FAQs found

Can you provide the shelf-life for the Mitochondria Isolation Kit for Cultured Cells?

The Mitochondria Isolation Kit for Cultured Cells is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is a typical yield from the Mitochondria Isolation Kit for Cultured Cells?

The typical yield is approximately 150 µg protein from 20 million starting cells after mitochondrial isolation and lysis, based on HeLa cells. This varies with cell type.

Can I use a Dounce homogenization procedure in conjunction with the Mitochondria Isolation Kit for Cultured Cells (Cat. No. 89874)?

Yes, the kit offers an optimized Dounce homogenization procedure, which results in two-fold greater mitochondri recovery compared to reagent-based method.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I further purify my crude mitochondrial fraction isolated using your Mitochondria Isolation Kit for Cultured Cells (Cat. No. 89874)?

To obtain a more purified fraction of heavy mitochondria, centrifuge the post-nuclear supernatant at 3000g rather than 12,000g. Western blot analysis of purified versus crude mitochondrial fractions prepared with this kit result in a > 50% reduction in cathepsin S (lysosomal protein) and PMP70 (peroxisomal protein) in the purified fraction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kits do you offer for cell fractionation?

We offer the following kits for cell fractionation:

Mem-PER Plus Membrane Protein Extraction Kit, Cat. No. 89842
NE-PER Nuclear and Cytoplasmic Extraction Reagents, Cat. No. 78835
Subcellular Protein Fractionation Kit for Cultured Cells, Cat. No. 78840
Subcellular Protein Fractionation Kit for Tissues, Cat. No. 87790
Mitochondrial Isolation Kit for Cultured Cells, Cat. No. 89874
Mitochondrial Isolation kit for Tissue, Cat. No. 89801
Lysosome Enrichment Kit for Tissues and Cultured Cells, Cat. No. 89839

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Why is cell fractionation important?

Isolation of subcellular fractions and concentration of low-abundantce proteins allows for more efficient identification and study of the proteins of interest.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.