Not just fat: investigating the proteome of cetacean blubber tissue.
AuthorsKershaw JL,Botting CH,Brownlow A,Hall AJ
JournalConservation physiology
PubMed ID29479430
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This ... More
Comparison of methods to isolate proteins from extracellular vesicles for mass spectrometry-based proteomic analyses.
AuthorsSubedi P,Schneider M,Philipp J,Azimzadeh O,Metzger F,Moertl S,Atkinson MJ,Tapio S
JournalAnalytical biochemistry
PubMed ID31401005
Extracellular vesicles (EVs) are cell-derived membrane-bound organelles that have generated interest as they reflect the physiological condition of their source. Mass spectrometric (MS) analyses of protein cargo of EVs may lead to the discovery of biomarkers for diseases. However, for a comprehensive MS-based proteomics analysis, an optimal lysis of the ... More
Alternative direct-to-amplification cell lysis techniques for forensically relevant non-sperm cells.
AuthorsArya R,Hudson BC,Green TD
JournalJournal of forensic sciences
PubMed ID37779342
While efforts have been made to reduce the pervasive backlog of sexual assault evidence collection kits, the actual laboratory process remains very time-consuming due to the requirement of a differential lysis step before DNA purification, as well as intricate mixture analysis towards the end of the DNA workflow. Recently, an ... More
TGFB1-induced extracellular expression of TGFBIp and inhibition of TGFBIp expression by RNA interference in a human corneal epithelial cell line.
AuthorsYellore VS, Rayner SA, Aldave AJ
JournalInvest Ophthalmol Vis Sci
PubMed ID20881301
'To report the increased production of extracellular transforming growth factor ß-induced protein (TGFBIp) by human corneal epithelial cells (HCECs) after induction by TGFB1 and the inhibition of TGFBIp production in induced and noninduced HCECs by RNA interference (RNAi).' ... More
Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs.
AuthorsPei M, He F, Boyce BM, Kish VL
JournalOsteoarthritis Cartilage
PubMed ID19128988
Synovium-derived stem cells (SDSCs) have proven to be superior in cartilage regeneration compared with other sources of mesenchymal stem cells. We hypothesized that conventionally passaged SDSCs can be engineered in vitro into cartilage tissue constructs and the engineered premature tissue can be implanted to repair allogeneic full-thickness femoral condyle cartilage ... More
Adult hippocampal neural stem and progenitor cells regulate the neurogenic niche by secreting VEGF.
AuthorsKirby ED, Kuwahara AA, Messer RL, Wyss-Coray T
Journal
PubMed ID25775598
The adult hippocampus hosts a population of neural stem and progenitor cells (NSPCs) that proliferates throughout the mammalian life span. To date, the new neurons derived from NSPCs have been the primary measure of their functional relevance. However, recent studies show that undifferentiated cells may shape their environment through secreted ... More
Trehalose, an mTOR-Independent Inducer of Autophagy, Inhibits Human Cytomegalovirus Infection in Multiple Cell Types.
AuthorsBelzile JP, Sabalza M, Craig M, Clark E, Morello CS, Spector DH
JournalJ Virol
PubMed ID26559848
Human cytomegalovirus (HCMV) is the major viral cause of birth defects and a serious problem in immunocompromised individuals and has been associated with atherosclerosis. Previous studies have shown that the induction of autophagy can inhibit the replication of several different types of DNA and RNA viruses. The goal of the ... More