The increase in global climate variability has increased the frequency and severity of floods, profoundly affecting agricultural production and food security worldwide. Autophagy is an intracellular catabolic pathway that is dispensable for plant responses to submergence. However, the physiological role of autophagy in plant response to submergence remains unclear. In ... More
Downregulation of STAT1 improved learning and memory impairments in aging mice.
AuthorsLi X,Xu Y,Li T,Xiong B,Yang X,Feng Y
JournalNeuroscience letters
PubMed ID39933634
Cognitive impairment is a typical hallmark of aging in mice and humans. Here, we reported that downregulation of STAT1 improved learning and memory impairments in aging mice by enhancing the expression of synaptic protein and inhibiting the expression of inflammatory factors. Proteomic analysis revealed 139 differentially expressed proteins (DEPs) in ... More
Tandem Mass Tags for Comparative and Discovery Proteomics.
AuthorsPagel O,Kollipara L,Sickmann A
JournalMethods in molecular biology (Clifton, N.J.)
PubMed ID33950487
Relative or comparative proteomics provides valuable insights about the altered protein abundances across different biological samples in a single (labeled) or series (label-free) of LC-MS measurement(s). Chemical labeling of peptides using isobaric mass tags for identification and quantification of different proteomes simultaneously has become a routine in the so-called discovery ... More
A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development
AuthorsLy J, Blengini CS, Cady SL, Schindler K, Cheeseman IM.
JournalCurr Biol
PubMed ID39178843
The chromosome segregation and cell division programs associated with somatic mitosis and germline meiosis display dramatic differences such as kinetochore orientation, cohesin removal, or the presence of a gap phase.(1)(,)(2)(,)(3)(,)(4)(,)(5)(,)(6) These changes in chromosome segregation require alterations to the established cell division machinery.(5)(,)(6) It remains unclear what aspects of kinetochore ... More
A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in Candida albicans.
AuthorsShivarathri R,Chauhan M,Mazumdar R,Trinh PC,Reiter W,Hartl M,Kuchler K,Chauhan N
JournalMethods in molecular biology (Clifton, N.J.)
PubMed ID36008655
Candida albicans is a normal component of the human microflora that colonizes mucosal/epithelial surfaces and the gastrointestinal tract as a commensal organism. However, in an immunocompromised host, it can cause life-threatening infections of high mortality and morbidity. Virulence as well as antifungal drug resistance of C. albicans is often regulated ... More
Urinary Proteomics for Discovery of Gastric Cancer Biomarkers to Enable Precision Clinical Oncology.
AuthorsJoshi N,Bhat F,Bellad A,Sathe G,Jain A,Chavan S,Sirdeshmukh R,Pandey A
JournalOmics : a journal of integrative biology
PubMed ID37579183
For precision in clinical oncology practice, detection of tumor-derived peptides and proteins in urine offers an attractive and noninvasive alternative for diagnostic or screening purposes. In this study, we report comparative quantitative proteomic profiling of urine samples from patients with gastric cancer and healthy controls using tandem mass tags-based multiplexed ... More
DiDBiT-TMT: A Novel Method to Quantify Changes in the Proteomic Landscape Induced by Neural Plasticity.
Direct detection of biotinylated proteins (DiDBiT) is a proteomic method that can enrich and detect newly synthesized proteins (NSPs) labeled with bio-orthogonal amino acids with 20-fold improved detectability compared to conventional methods. However, DiDBiT has currently been used to compare only two conditions per experiment. Here, we present DiDBiT-TMT, a ... More
Proteomics of plasma-derived extracellular vesicles reveals S100A8 as a novel biomarker for Alzheimer's disease: A preliminary study.
AuthorsZhang Y,Zhao Y,Zhang J,Gao Y,Gao X,Li S,Chang C,Yang G
JournalJournal of proteomics
PubMed ID39159863
Extracellular vesicles (EVs) act as mediators for intercellular transfer of Aβ and tau proteins, promoting the propagation of these pathological misfolded proteins throughout the brain in Alzheimer's disease (AD). Levels of blood exosomal Aβ(42), total Tau (t-Tau) and phosphorylated Tau (p-Tau) had a high correlation with their concentrations in cerebrospinal ... More