PrimeView Human Gene Expression Array
PrimeView Human Gene Expression Array
Applied Biosystems™

PrimeView Human Gene Expression Array

The PrimeView Human Gene Expression Array enables you to: • Measure gene expression of more than 36,000 transcripts and variantsRead more
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Catalog NumberNumber of Arrays
90183830 arrays
90183710 arrays
Catalog number 901838
Price (CNY)
-
Number of Arrays:
30 arrays
The PrimeView Human Gene Expression Array enables you to:
• Measure gene expression of more than 36,000 transcripts and variants per sample
• Get accurate and reproducible gene expression data by using multiple independent measurements for each transcript

Proven performance from the industry standard
The GeneChip PrimeView Human Gene Expression Array cartridge enables expression profiling using probe sets with an emphasis on established, well annotated content. Sequences used in the design of the array were selected from the RefSeq version 36, UniGene database 219, and full-length human mRNAs from GenBank™.

Key benefits
• Offers excellent gene expression specificity and reproducibility
• Produces reliable results with multiple independent measurements per transcript — 11 probes per set for well-annotated sequences, 9 probes per set for the remainder
• Provides complete coverage of the annotated genome
• Highest transcript coverage in the industry with the most recent annotation — covers all well- annotated genes and transcripts from RefSeq

Content profile
• The PrimeView Human Gene Expression Array is comprised of more than 530,000 probes covering more than 36,000 transcripts and variants, which represent more than 20,000 genes mapped through RefSeq or via UniGene annotation.
• The EST and mRNA sequences used in the design were clustered and assembled to create consensus sequences that represent alternative splice forms. Each assembly was then analyzed for orientation and alternative 3' end evidence.
• Content was chosen to cover all well-annotated genes and transcripts from RefSeq v36 and by leveraging all available EST and mRNA evidence that fall into the same clusters, to rigorously detect alternate 3' ends of those well-annotated genes.
• More than 1,000 probe sets represent transcripts that have no official gene symbol in UniGene, but are based on predicted RefSeq sequences and UniGene clusters with good evidence of actual transcription.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity30 arrays
TypeHuman Gene Expression Array
ArrayTranscriptome Profiling
FormatArray Cartridge
Number of Arrays30 arrays
SpeciesHuman
Unit SizeEach

Frequently asked questions (FAQs)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How are transcript IDs assigned for WT arrays if a gene has multiple transcript IDs?

The first assigned mRNA/gene that is listed in the annotation file for a given probe set or transcript cluster is considered the "best" assignment based on data source quality ranking and assignment scoring system. To get a single best mRNA/gene assignment you can simply take the first one in the list in the annotation file.

Sometimes, there might be multiple best hits between transcript clusters (or probe set) and a mRNA/gene, with identical score and data source quality. In that case, the transcript assignments are ranked based on their descriptions (e.g., title from the GenBank record).

For transcript clusters that detect genes that are alternatively spliced, the different spliced forms will be ranked by length with the longest ones listed first.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is an example of using ERCC controls in the PrimeView Human Gene Expression Array?

The following link is an example of how the ERCC control probes on the Primeview Human Gene Expression Array can be used: https://pubmed.ncbi.nlm.nih.gov/23101621/.
Please note that the ERCC controls, while tiled on the array itself, are not visible in the commercial library files. A CDF file does exist with these made visible, however. Please contact Technical Support to access a copy of this.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does the GeneChip miRNA 3.1 Array Strip require a different hyb/wash/stain kit than the GeneChip 3' IVT arrays and GeneChip ST array strips?

The hyb/wash/stain kit for the GeneChip miRNA 3.1 Array Strips is different than that for the 3' IVT arrays and GeneChip ST array strips. Additional wash A steps have been added to ensure adequate washing after the array strips have been stained.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Are the PrimeView Human Genome U219 Array Strip and PrimeView Human Genome U219 Array Plate the same as the PrimeView Human Gene Expression Array?

Yes, the PrimeView Human U219 Array and the Primeview Human Gene Expression Array are indeed the same array (including strip and plate).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.