PrimeView Human Gene Expression Array, 30 arrays - FAQs

View additional product information for PrimeView Human Gene Expression Array - FAQs (901838, 901837)

7 product FAQs found

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How are transcript IDs assigned for WT arrays if a gene has multiple transcript IDs?

The first assigned mRNA/gene that is listed in the annotation file for a given probe set or transcript cluster is considered the "best" assignment based on data source quality ranking and assignment scoring system. To get a single best mRNA/gene assignment you can simply take the first one in the list in the annotation file.

Sometimes, there might be multiple best hits between transcript clusters (or probe set) and a mRNA/gene, with identical score and data source quality. In that case, the transcript assignments are ranked based on their descriptions (e.g., title from the GenBank record).

For transcript clusters that detect genes that are alternatively spliced, the different spliced forms will be ranked by length with the longest ones listed first.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is an example of using ERCC controls in the PrimeView Human Gene Expression Array?

The following link is an example of how the ERCC control probes on the Primeview Human Gene Expression Array can be used: https://pubmed.ncbi.nlm.nih.gov/23101621/.
Please note that the ERCC controls, while tiled on the array itself, are not visible in the commercial library files. A CDF file does exist with these made visible, however. Please contact Technical Support to access a copy of this.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does the GeneChip miRNA 3.1 Array Strip require a different hyb/wash/stain kit than the GeneChip 3' IVT arrays and GeneChip ST array strips?

The hyb/wash/stain kit for the GeneChip miRNA 3.1 Array Strips is different than that for the 3' IVT arrays and GeneChip ST array strips. Additional wash A steps have been added to ensure adequate washing after the array strips have been stained.

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Are the PrimeView Human Genome U219 Array Strip and PrimeView Human Genome U219 Array Plate the same as the PrimeView Human Gene Expression Array?

Yes, the PrimeView Human U219 Array and the Primeview Human Gene Expression Array are indeed the same array (including strip and plate).

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What do the suffixes mean at the end of each probeset in GeneChip 3' IVT arrays?

The probe set names never change, but they can give you an idea of what was known about the sequence at the time of design.
_at: All probes hit one known transcript.
_a: All probes in the set hit alternate transcripts from the same gene.
_s: All probes in the set hit transcripts from different genes.
_x: Some probes hit transcripts from different genes.

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Can I store my GeneChip 3' IVT Array after hybridization?

Yes, but we recommend storing your GeneChip 3' IVT Array after hybridization and prior to the fluidics step, for no longer than 3 hours at 4 degrees C. Please remove the hybridization cocktail and fill with wash A before storing.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.