OncoScan™ CNV 检测试剂盒
OncoScan™ CNV 检测试剂盒
Applied Biosystems™

OncoScan™ CNV 检测试剂盒

OncoScan CNV 检测试剂盒是一种基于全基因组拷贝数微阵列技术的检测方法，能够检测相关的拷贝数变异 (CNV)，如拷贝数增加和减少、杂合性缺失（loss of heterozygosity，以下简称 LOH）了解更多信息
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货号数量
90269524 samples
货号 902695
价格（CNY)
-
数量:
24 samples
OncoScan CNV 检测试剂盒是一种基于全基因组拷贝数微阵列技术的检测方法，能够检测相关的拷贝数变异 (CNV)，如拷贝数增加和减少、杂合性缺失（loss of heterozygosity，以下简称 LOH）、拷贝数平衡杂合性缺失（copy neutral loss of heterozygosity，以下简称 cnLOH）、倍性、等位基因特定变化、断裂点测定、嵌合、克隆异质性和染色体碎裂。它具有与 OncoScan CNV Plus 检测试剂盒相同的拷贝数覆盖度，但不覆盖体细胞突变。该试剂盒提供了福尔马林固定石蜡包埋 (FFPE) 肿瘤样品、微阵列芯片杂交及染色等样品制备步骤所需的试剂。OncoScan CNV 检测试剂盒覆盖整个基因组以及已知的癌症驱动基因，作为整个检测工作流程的一部分，它可以从每个样品中低至 80 ng 的 DNA 中生成深度拷贝数数据。

OncoScan CNV 检测试剂盒的主要特点包括：
•全基因组拷贝数分析—检测基因结构变异，如缺失、重复、LOH、cnLOH、断裂点测定、倍性、嵌合和不平衡易位，这些变异通过短读长测序或靶向测序不能很好地分析
• 全面覆盖—对癌症和肿瘤进展中具有确定意义和重要性的基因以及有新证据的基因进行全基因组分析，有助于“在未来验证”技术投入并较大限度地减少再验证负担
• 灵活性高—仅需一次检测就可确定染色体臂畸变、局部突变、LOH 和 cnLOH，有助于降低成本，缩短处理时间
• 可靠的性能—不同批次之间以及不同操作员之间均可得出标准化结果
• 起始样本量少，可快速得出结果—仅需 80 ng DNA（FFPE 来源），且能在 72 小时内获得结果
• 快速分析—免费软件可在数分钟内将数百个样本的数据直观显示出来
• 在特定癌症基因中进行高分辨率拷贝数检测—准确识别极小拷贝数 (50–125 kb) 至大 (Mb) 拷贝数范围内的变异

覆盖范围和性能
• ˜900 个癌症基因中 50–100 kb 拷贝数分辨率
• 癌症基因之外的 300 kb 全基因组拷贝数分辨率
• 全基因组 LOH 检测包括 cnLOH 检测
• 10+ 个拷贝数的高动态范围
• 证实为 FISH 确认的关键癌症基因（包括 ERBB2 (Her2)、EGFR、MDM2、MYC 和 FGFR1）扩增片段

分析软件
数据分析免费且简单易用，可选用三种分析软件中的一种：
•Chromosome Analysis Suite (ChAS)，对于少数样品的拷贝数调用
• Nexus Express 软件，对于数十到数百个样本的拷贝数和体细胞突变调用

仅供科研使用。不可用于诊断程序。
规格
包括OncoScan CNV 阵列（货号：902694），OncoScan CNV 试剂盒（货编号 902692TS）
样品数量24 samples
标记物数量220,000 个以上 SNP
数量24 samples
类型CNV 检测
数组细胞遗传学, 拷贝数
产品规格Genechip 探针阵列
阵列数量48 个阵列/24 个样品
Unit SizeEach
内容与储存
•OncoScan CNV 阵列（货号 902694）
• OncoScan CNV 试剂盒（货号 902692TS）

常见问题解答 (FAQ)

Can I view OncoScan CHP (OSCHP) files from both OncoScan CNV Assay (Cat. No. 902293) and OncoScan CNV Plus Assay (Cat. No. 902695) simultaneously, in Chromosome Analysis Suite (ChAS) Software?

Yes, OSCHP files from both OncoScan CNV Assay (Cat. No. 902293) and OncoScan CNV Plus Assay (Cat. No. 902695) can be opened and viewed simultaneously in Chromosome Analysis Suite (ChAS) Software.
Note: The OncoScan CNV Assay does not contain the somatic mutation probes that are in the OncoScan CNV Plus Assay.

What is the difference between the OncoScan CNV Plus Assay (Cat. No. 902293) and the OncoScan CNV Assay (Cat. No. 902695)?

The main difference between the two products is in the reagent kit. The OncoScan CNV Plus Assay contains both somatic mutation probes and copy number probes. The OncoScan CNV Assay contains only copy number probes.

- The OncoScan CNV Plus Reagent Kit contains the OncoScan CNV Plus Somatic Mutation Probe Mix 1.0 (Part No. 902247).
- The OncoScan CNV Reagent Kit does not contain the Somatic Mutation Probe Mix. Instead, it contains Buffer C (Part No. 902696).

The OncoScan CNV Plus Assay detects genome-wide copy number changes, loss of heterozygosity (LOH), and a panel of somatic mutations. The somatic mutation panel includes 74 mutations found in BRAF, KRAS, EGFR, IDH1, IDH2, PTEN, PIK3CA, NRAS, and TP53.

The OncoScan CNV Assay detects genome-wide copy number changes and loss of heterozygosity (LOH).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the format size and fill volume for Oncoscan CNV Assay (Cat. No. 902695)?

Oncoscan CNV Assay is 8 micron, 100 format. 160 µL Hyb mix is added to the array. There should be no air bubble after the hyb mix is added and the chamber should be filled completely.

Is there an equivalent metric for the "Offset Flag" in the QC and Sample Info tab in Chromosome Analysis Suite (ChAS) for OncoScan?

There is no available equivalent to the "Offset Flag" in the QC and Sample Info tab in ChAS. The "Offset Flag" is a QC metric that is found in the Analysis Dashboard for OncoScan products. This QC metric lets us know if the TuScan algorithm detected that the usual normalization method was off, and therefore required readjustment.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why is it important to use Tough-Spots® labels when using GeneChip cartridge arrays?

Tough-Spots® labels are small adhesive stickers used to temporarily seal the backs of cartridge arrays during the overnight hybridization step. They are required to prevent loss of volume due to evaporation through the septa. We recommend using Tough-Spots® labels on Rolls from USA Scientific (Item No. 9185-0000)

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

View all OncoScan™ CNV 检测试剂盒 FAQs