IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.
Authors:Blom L, Poulsen LK,
Journal:J Immunol
PubMed ID:23028054
'The IL-1 family members IL-1ß, IL-18, and IL-33 are potent cytokines in relationship to amplifying the CD4(+) T cell cytokine production. To evaluate their impact on in vitro-differentiated human Th1 and Th2 cultures, such cultures were established from naive T cells, purified from healthy blood donors, and reactivated in the ... More
Multiparameter flow cytometry and bioanalytics for B cell profiling in systemic lupus erythematosus.
Authors:Kaminski DA, Wei C, Rosenberg AF, Lee FE, Sanz I
Journal:Methods Mol Biol
PubMed ID:22933067
B lymphocyte involvement in systemic lupus erythematosus has been recognized for several decades, mainly in the context of autoantibody production. Both mouse and human studies reveal that different types of antibody responses, as well as antibody-independent effector functions can be ascribed to distinct subpopulations (subsets) of circulating B cells. Characterizing ... More
Evaluation of pneumonia virus of mice as a possible human pathogen.
Pneumonia virus of mice (PVM), a relative of human respiratory syncytial virus (RSV), causes respiratory disease in mice. There is serologic evidence suggesting widespread exposure of humans to PVM. To investigate replication in primates, African green monkeys (AGM) and rhesus macaques (n = 4) were inoculated with PVM by the ... More
A novel aldehyde dehydrogenase-3 activator leads to adult salivary stem cell enrichment in vivo.
Authors:Banh A, Xiao N, Cao H, Chen CH, Kuo P, Krakow T, Bavan B, Khong B, Yao M, Ha C, Kaplan MJ, Sirjani D, Jensen K, Kong CS, Mochly-Rosen D, Koong AC, Le QT,
Journal:Clin Cancer Res
PubMed ID:21998334
To assess aldehyde dehydrogenase (ALDH) expression in adult human and murine submandibular gland (SMG) stem cells and to determine the effect of ALDH3 activation in SMG stem cell enrichment. Adult human and murine SMG stem cells were selected by cell surface markers (CD34 for human and c-Kit for mouse) and ... More
Targeting GLUT1 and the Warburg effect in renal cell carcinoma by chemical synthetic lethality.
Authors:Chan DA, Sutphin PD, Nguyen P, Turcotte S, Lai EW, Banh A, Reynolds GE, Chi JT, Wu J, Solow-Cordero DE, Bonnet M, Flanagan JU, Bouley DM, Graves EE, Denny WA, Hay MP, Giaccia AJ,
Journal:Sci Transl Med
PubMed ID:21813754
Identifying new targeted therapies that kill tumor cells while sparing normal tissue is a major challenge of cancer research. Using a high-throughput chemical synthetic lethal screen, we sought to identify compounds that exploit the loss of the von Hippel-Lindau (VHL) tumor suppressor gene, which occurs in about 80% of renal ... More