Novel Live Alkaline Phosphatase Substrate for Identification of Pluripotent Stem Cells.
AuthorsSingh U, Quintanilla RH, Grecian S, Gee KR, Rao MS, Lakshmipathy U,
JournalStem Cell Rev
PubMed ID22426885
Characterization of pluripotent stem cells.
AuthorsMartí M, Mulero L, Pardo C, Morera C, Carrió M, Laricchia-Robbio L, Esteban CR, Izpisua Belmonte JC,
JournalNat Protoc
PubMed ID23306458
Characterization of pluripotent stem cells is required for the registration of stem cell lines and allows for an impartial and objective comparison of the results obtained when generating multiple lines. It is therefore crucial to establish specific, fast and reliable protocols to detect the hallmarks of pluripotency. Such protocols should ... More
Immune Defense Protein Expression in Highly Purified Mouse Lung Epithelial Cells.
AuthorsSinha M, Lowell CA
JournalAm J Respir Cell Mol Biol
PubMed ID26574781
Lung epithelial cells play critical roles in initiating and modulating immune responses during pulmonary infection or injury. To better understand the spectrum of immune response-related proteins present in lung epithelial cells, we developed an improved method of isolating highly pure primary murine alveolar type (AT) II cells and murine tracheal ... More