Flow cytometric measurement of the production of reactive oxygen intermediate in activated rat mast cells.
AuthorsTsinkalovsky OR, Laerum OD
JournalAPMIS
PubMed ID7520710
The production of reactive oxygen intermediates (ROI) in compound 48/80- or calcium ionophore A 23187- activated pleural or peritoneal mast cells was monitored using flow cytometry and the fluorescence indicator dihydrorhodamine 123 (a derivate of rhodamine 123). Mast cell degranulation and ROI production were estimated by flow cytometric fluorescence and ... More
Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds.
AuthorsDiatchuk V, Lotan O, Koshkin V, Wikstroem P, Pick E
JournalJ Biol Chem
PubMed ID9148950
The elicitation of an oxidative burst in phagocytes rests on the assembly of a multicomponental complex (NADPH oxidase) consisting of a membrane-associated flavocytochrome (cytochrome b559), representing the redox element responsible for the NADPH-dependent reduction of oxygen to superoxide (O-2), two cytosolic components (p47(phox), p67(phox)), and the small GTPase Rac (1 ... More
Exocytotic insertion of calcium channels constrains compensatory endocytosis to sites of exocytosis.
AuthorsSmith RM, Baibakov B, Ikebuchi Y, White BH, Lambert NA, Kaczmarek LK, Vogel SS
JournalJ Cell Biol
PubMed ID10684256
'Proteins inserted into the cell surface by exocytosis are thought to be retrieved by compensatory endocytosis, suggesting that retrieval requires granule proteins. In sea urchin eggs, calcium influx through P-type calcium channels is required for retrieval, and the large size of sea urchin secretory granules permits the direct observation of ... More
Involvement of nuclear factor of activated T cells activation in UV response. Evidence from cell culture and transgenic mice.
AuthorsHuang C, Mattjus P, Ma WY, Rincon M, Chen NY, Brown RE, Dong Z
JournalJ Biol Chem
PubMed ID10734048
'Mammalian cells respond to UV radiation by signaling cascades leading to activation of transcription factors, such as activated protein 1, NFkappaB, and p53, a process known as the "UV response." Nuclear factor of activated T cells (NFAT) was first identified as an inducible nuclear factor in immune response and subsequently ... More
Potentiation of CD3-induced expression of the linker for activation of T cells (LAT) by the calcineurin inhibitors cyclosporin A and FK506.
AuthorsPeters D, Tsuchida M, Manthei ER, Alam T, Cho CS, Knechtle SJ, Hamawy MM
JournalBlood
PubMed ID10779414
'The activation of blood cells, including T cells, triggers intracellular signals that control the expression of critical molecules, including cytokines and cytokine receptors. We show that T-cell receptor (TCR) ligation increases the cellular level of the protein linker for activation of T cells (LAT), a molecule critical for T-cell development ... More
Galpha11 induces caspase-mediated proteolytic activation of Rho-associated kinase, ROCK-I, in HeLa cells.
'Expression of the constitutively active mutant of Galpha(11) (Galpha(11)QL) induces the formation of vinculin-containing focal adhesion-like structures in HeLa cells. This was found to be inhibited by Y-27632, a specific inhibitor of Rho-associated kinases (ROCK), but not by co-expression with a dominant negative mutant of RhoA, suggesting Rho-independent activation of ... More
Regulatory role of CD4/L3T4 molecules in IL-2 production by affecting intracellular Ca2+ concentration of T cell clone stimulated with soluble anti-CD3.
AuthorsTamura T, Mizuguchi J, Nariuchi H
JournalJ Immunol
PubMed ID1972723
'To elucidate the role of CD4 molecule in T cell activation, the effect of anti-CD4 on T cell IL-2 production was examined by using an alloreactive Th clone. The alloreactive T cell used in the present experiments produced IL-2 in response to soluble anti-CD3 epsilon-chain (anti-CD3) without accessory cell or ... More
Differential localization of 5- and 15-lipoxygenases to the nuclear envelope in RAW macrophages.
AuthorsChristmas P, Fox JW, Ursino SR, Soberman RJ
JournalJ Biol Chem
PubMed ID10464294
'Leukotriene formation is initiated in myeloid cells by an increase in intracellular calcium and translocation of 5-lipoxygenase from the cytoplasm to the nuclear envelope where it can utilize arachidonic acid. Monocyte- macrophages and eosinophils also express 15-lipoxygenase, which converts arachidonic acid to 15(S)-hydroxyeicosatetraenoic acid. Enhanced green fluorescent 5-lipoxygenase (5-LO) and ... More
Isosteric metal complexes of ionophore A23187: a basis for cation selectivity.
AuthorsPfeiffer DR, Deber CM
JournalFEBS Lett
PubMed ID385347
Prostaglandin E2 stimulates a Ca2+-dependent K+ channel in human erythrocytes and alters cell volume and filterability.
AuthorsLi Q, Jungmann V, Kiyatkin A, Low PS
JournalJ Biol Chem
PubMed ID8702518
'To understand the mechanism by which human red blood cells (RBCs) contribute to hemostasis and thrombosis, we have examined the effects of metabolites released by activated platelets on intact RBCs. Prostaglandin E2 (PGE2), a signal molecule produced by activated platelets, was observed to lower the filterability of human erythrocytes by ... More
Proteolytic processing of Alzheimer's disease beta A4 amyloid precursor protein in human platelets.
AuthorsLi QX, Evin G, Small DH, Multhaup G, Beyreuther K, Masters CL
JournalJ Biol Chem
PubMed ID7775475
'The processing of amyloid precursor protein (APP) and production of beta A4 amyloid are events likely to influence the development and progression of Alzheimer''s disease, since beta A4 is the major constituent of amyloid deposited in this disorder. Our previous studies showed that human platelets contain full-length APP (APPFL) and ... More
In vivo desensitization of glycogenolysis to Ca2+-mobilizing hormones in rat liver cells.
AuthorsTsujimoto G, Tsujimoto A, Kato K, Hashimoto K
JournalJ Clin Invest
PubMed ID2848864
'Rat hepatocytes contain several types of Ca2+-linked receptors, all of which stimulate glycogen breakdown by increasing cytosolic free Ca2+ concentration [( Ca2+]c). In vivo desensitization of this Ca2+ messenger system was studied in hepatocytes isolated from either pheochromocytoma (PHEO)-harboring and chronically norepinephrine (NE)-infused rats. Homologous desensitization for alpha 1-adrenergic receptor-mediated ... More
Regulation of cytosolic free calcium concentration by intrasynaptic mitochondria.
AuthorsMartínez-Serrano A, Satrústegui J
JournalMol Biol Cell
PubMed ID1550964
'By the use of digitonin permeabilized presynaptic nerve terminals (synaptosomes), we have found that intrasynaptic mitochondria, when studied "in situ," i.e., surrounded by their cytosolic environment, are able to buffer calcium in a range of calcium concentrations close to those usually present in the cytosol of resting synaptosomes. Adenine nucleotides ... More
Elimination of the hydroxyl groups in the ribose ring of ATP reduces its ability to phosphorylate the sarcoplasmic reticulum Ca(2+)-ATPase.
AuthorsCoan C, Amaral Júnior JA, Verjovski-Almeida S
JournalJ Biol Chem
PubMed ID8463222
'2''-Deoxyadenosine 5''-triphosphate, 3''-deoxyadenosine 5''-triphosphate, and 3''-amino-3''-deoxyadenosine 5''-triphosphate were substituted for ATP in the Ca2+ pumping cycle of the sarcoplasmic reticulum Ca(2+)-ATPase. The rate of phosphorylation of the enzyme decreased by more than an order of magnitude when either of the hydroxyl groups was eliminated from the ribose ring. This resulted ... More
A prelysosomal compartment sequesters membrane-impermeant fluorescent dyes from the cytoplasmic matrix of J774 macrophages.
AuthorsSteinberg TH, Swanson JA, Silverstein SC
JournalJ Cell Biol
PubMed ID3417786
'After the membrane impermeant dye Lucifer Yellow is introduced into the cytoplasmic matrix of J774 cells, the dye is sequestered within cytoplasmic vacuoles and secreted into the extracellular medium. In the present work we studied the intracellular transport of Lucifer Yellow in J774 macrophages and the nature of the cytoplasmic ... More
Beta-adrenergic modulation of the polymorphonuclear leukocyte respiratory burst is dependent upon the mechanism of cell activation.
AuthorsNielson CP
JournalJ Immunol
PubMed ID2821113
'Although inhibition of polymorphonuclear leukocyte activation by beta-adrenoceptor agonists has been recognized for over a decade, effects have only been observed at high drug concentrations and in the presence of theophylline. In this study, catecholamine and prostaglandin modulation of the respiratory burst was evaluated with respect to the mechanism of ... More
Prostacyclin is a specific effector of adipose cell differentiation. Its dual role as a cAMP- and Ca(2+)-elevating agent.
AuthorsVassaux G, Gaillard D, Ailhaud G, Négrel R
JournalJ Biol Chem
PubMed ID1317853
'The mitogenic-adipogenic activity of carbaprostacyclin (cPGI2), a stable analogue of prostacyclin (PGI2), has been proposed to be related to its ability to elicit cAMP production and to activate the protein kinase A cascade (Négrel, R., Gaillard, D., and Ailhaud, G. (1989) Biochem. J. 257, 399-405). In the present study, cPGI2 ... More
Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets.
AuthorsGreimers R, Trebak M, Moutschen M, Jacobs N, Boniver J
JournalCytometry
PubMed ID8974866
'A visible-light, dual-laser, flow cytometric method was developed for the simultaneous analysis of intracellular ionized calcium concentration ([Ca2+]i) and three cell-surface markers (CD4, CD8, and Thy-1.2 antigens) by using the calcium probe fluo-3 and using R-phycoerythrin (PE), peridinin chlorophyll-alpha protein (PerCP), and allophycocyanin (APC) conjugated monoclonal antibodies (MoAbs). This improved ... More
Actin assembly in electropermeabilized neutrophils: role of intracellular calcium.
AuthorsDowney GP, Chan CK, Trudel S, Grinstein S
JournalJ Cell Biol
PubMed ID2112547
'Assembly of microfilaments involves the conversion of actin from the monomeric (G) to the filamentous (F) form. The exact sequence of events responsible for this conversion is yet to be defined and, in particular, the role of calcium remains unclear. Intact and electropermeabilized human neutrophils were used to assess more ... More
Ca2+ mobilization primes protein kinase C in human platelets. Ca2+ and phorbol esters stimulate platelet aggregation and secretion synergistically through protein kinase C.
AuthorsSiess W, Lapetina EG
JournalBiochem J
PubMed ID3143357
'Low concentrations of Ca2+-mobilizing agonists such as vasopressin, platelet-activating factor, ADP, the endoperoxide analogue U44069 and the Ca2+ ionophore A23187 enhance the binding of [3H]phorbol 12,13-dibutyrate (PdBu) to intact human platelets. This effect is prevented by preincubation of platelets with prostacyclin (except for A23187). Adrenaline, which does not increase Ca2+ ... More
Distribution and dynamics of mouse sperm surface galactosyltransferase: implications for mammalian fertilization.
AuthorsCardullo RA, Wolf DE
JournalBiochemistry
PubMed ID7632675
'It has been proposed that a mouse sperm surface beta-1,4-galactosyltransferase functions as a receptor for the zona pellucida during fertilization. In this paper we used two monovalent fluorescent probes specific for galactosyltransferase: a trinitrophenylated derivative of UDP-galactose and rhodaminated alpha-lactalbumin. We found that galactosyltransferase was initially present over the posterior ... More
Prostacyclin inhibits platelet aggregation induced by phorbol ester or Ca2+ ionophore at steps distal to activation of protein kinase C and Ca2+-dependent protein kinases.
AuthorsSiess W, Lapetina EG
JournalBiochem J
PubMed ID2494992
'Suspensions of aspirin-treated, 32P-prelabelled, washed platelets containing ADP scavengers in the buffer were activated with either phorbol 12,13-dibutyrate (PdBu) or the Ca2+ ionophore A23187. High concentrations of PdBu (greater than or equal to 50 nM) induced platelet aggregation and the protein kinase C (PKC)-dependent phosphorylation of proteins with molecular masses ... More
Regulation of free and bound magnesium in rat hepatocytes and isolated mitochondria.
AuthorsCorkey BE, Duszynski J, Rich TL, Matschinsky B, Williamson JR
JournalJ Biol Chem
PubMed ID3081495
'Null point titration techniques have been developed for measurements of cytosolic free Mg2+ in isolated cells and matrix free Mg2+ in isolated mitochondria using antipyrylazo III as a spectrophotometric Mg2+ indicator. A cytosolic free Mg2+ of 0.37 +/- 0.02 mM was obtained with hepatocytes. This represented about 6% of the ... More
Calcium pretreatment induces the decrease in epidermal growth factor binding through the activation of transglutaminase in isolated liver membrane.
AuthorsKatoh S, Hashimoto M, Kohno H, Ohkubo Y
JournalArch Biochem Biophys
PubMed ID8099784
'Pretreatment of hepatocytes with Ca2+ and A23187 resulted in the decrease in epidermal growth factor (EGF) binding to the receptor. This decrease could be induced by only the pretreatment of the isolated liver membrane with Ca2+. By the pretreatment with 1 mM Ca2+ for 30 min, EGF binding was decreased ... More
Superoxide anion impairs contractility in cultured aortic smooth muscle cells.
AuthorsKimura C, Cheng W, Hisadome K, Wang YP, Koyama T, Karashima Y, Oike M, Ito Y
JournalAm J Physiol Heart Circ Physiol
PubMed ID12063312
'We examined the effects of superoxide anion (O) generated by xanthine plus xanthine oxidase (X/XO) on the intracellular Ca(2+) concentration ([Ca(2+)](i)) and muscle contractility in cultured bovine aortic smooth muscle cells (BASMC). Cells were grown on collagen-coated dish for the measurement of [Ca(2+)](i). Pretreatment with X/XO inhibited ATP-induced Ca(2+) transient ... More
Intracellular pH and the increase in protein synthesis accompanying activation of Xenopus eggs.
AuthorsGrandin N, Charbonneau M
JournalBiol Cell
PubMed ID2620165
'Metabolic activation following egg fertilization corresponds to an increase in protein synthesis and the initiation of DNA synthesis, which lead to cell division and development of the embryo. Since in several biological systems protein synthesis is regulated by intracellular pH (pHi), we have decided to investigate the situation during Xenopus ... More
The Na+/Ca2+ exchanger regulates cytolysin/perforin-induced increases in intracellular Ca2+ and susceptibility to cytolysis.
AuthorsKraut RP, Bose R, Cragoe EJ, Greenberg AH
JournalJ Immunol
PubMed ID1560205
'The Ca2+ dependency of NK cell-mediated and cytolysin-mediated cytolysis may be related to increases in target cell intracellular Ca2+. In a previous study we hypothesized that the Na+/Ca2+ exchanger can act as a counter-lytic mechanism by regulating the damaging increases in intracellular free calcium ([Ca2+]i) produced by cytolysin. We found ... More
Stimulation of lipid peroxidation increases the intracellular calcium content of isolated hepatocytes.
AuthorsAlbano E, Bellomo G, Parola M, Carini R, Dianzani MU
JournalBiochim Biophys Acta
PubMed ID2001414
'Lipid peroxidation induced in isolated rat hepatocytes by FeCl3 (0.1 mM) was associated with an increase in the cytosolic free Ca2+ and in the ionophore-mobilizable Ca2+ content of both mitochondrial and extramitochondrial (endoplasmic reticular) pools. Ca2+ accumulation was completely prevented by the antioxidants promethazine and vitamin E succinate and was ... More
Inhibition of HSP70 expression by calcium ionophore A23187 in human cells. An effect independent of the acquisition of DNA-binding activity by the heat shock transcription factor.
AuthorsElia G, De Marco A, Rossi A, Santoro MG
JournalJ Biol Chem
PubMed ID8663271
'Heat shock proteins (HSPs) are induced in mammalian cells in a variety of pathophysiological states and have an important role in cytoprotection in vitro and in vivo. In this study, we report that the calcium ionophore A23187, a glucose-regulated protein (GRP) inducer, dramatically inhibits HSP70 synthesis and HSP70 mRNA transcription ... More
The difference between platelet and plasma FXIII used to study the mechanism of platelet microvesicle formation.
AuthorsHolme PA, Brosstad F, Solum NO
JournalThromb Haemost
PubMed ID8115996
'The formation of microvesicles from platelets was induced either by activation of the complement system by a monoclonal antibody to CD9, or by incubation of platelets with the calcium ionophore A23187. A filter technique to isolate the microvesicles without plasma contamination is described. The microvesicles contained FXIIIa2 from the platelet ... More
Concanavalin A- and calcium-dependent phosphorylation of a protein of 80 kDa in mouse lymphocytes rendered permeable to exogenously added [gamma-32P]ATP.
AuthorsDenis GV, Toyoshima S, Osawa T
JournalBiochim Biophys Acta
PubMed ID3947677
'The phosphorylation of a protein of 80 kDa in permeable mouse lymphocytes is shown to be dependent both on exogenously added calcium and on concanavalin A. Lymphocyte plasma membranes are rendered permeable to exogenously added [gamma-32P]ATP and other small molecules by treatment with 20 micrograms/ml alpha-lysophosphatidylcholine for 1 min on ... More
A new method to detect acrosome-reacted spermatozoa using biotinylated soybean trypsin inhibitor.
AuthorsArts EG, Kuiken J, Jager S
JournalFertil Steril
PubMed ID7926117
'OBJECTIVE: To develop a method to detect acrosome-reacted spermatozoa on human zonae pellucidae using only commercially available reagents and without need for sperm fixation. DESIGN: Sperm head labeling with biotinylated soybean trypsin inhibitor (SBTI-biotin) was compared with results of a known method using fluorescein isothiocyanate (FITC)-conjugated Pisum sativum agglutinin. The ... More
Inhibitory effect of the intrauterine infusion of phorbol 12-myristate 13-acetate and 1-oleoyl-2-acetylglycerol on the decidual cell reaction in rats.
AuthorsFeyles V, Kennedy TG
JournalBiol Reprod
PubMed ID3115327
'In rats, prostaglandins (PGs) have an essential role in the decidual cell reaction (DCR), but their mechanism of action at the cellular level within the endometrium is at present uncertain. To test the hypothesis that both protein kinase C activation and calcium mobilization mediate the action of PGs within the ... More
Intracellular Ca2+ concentration and the antidiuretic hormone-induced increase in water permeability: effects of ionophore A23187 and quinidine.
AuthorsParisi M, Ibarra C, Porta M
JournalBiochim Biophys Acta
PubMed ID2825786
'The hydroosmotic responses induced by oxytocin and 8-bromo-cyclic AMP, in frog and toad urinary bladders, were recorded minute by minute. 3HHO and 45Ca unidirectional fluxes as well as prostaglandin B2 liberation were also measured. It was observed that: (1) Addition of the calcium ionophore A23187 or quinidine to the serosal ... More
Epidermal growth factor stimulation of prostacyclin production by cultured aortic smooth muscle cells: requirement for increased cellular calcium levels.
AuthorsBlay J, Hollenberg MD
JournalJ Cell Physiol
PubMed ID2544610
'We have examined the ability of epidermal growth factor (EGF) to regulate prostacyclin production by cultured A10 smooth muscle cells. EGF by itself had no effect on prostacyclin production, but it augmented the response to arg8-vasopressin. An AGF stimulation of prostacyclin production was also observed in the presence of the ... More
Ca(2+)-mediated damage to rabbit gastric mucosal cells: modulation by nitric oxide.
AuthorsTepperman BL, Soper BD
JournalEur J Pharmacol
PubMed ID8666044
'Pertubations in cellular Ca2+ homeostasis can lead to oxidative stress whereas nitric oxide has been shown to inactivate oxygen radicals. Therefore the effects of inhibition of nitric oxide (NO) synthase activity on Ca2+-mediated disruption to rabbit dispersed gastric mucosal cells have been examined. Addition of the Ca2+ ionophore A23187 (3-25 ... More
Production of hydrogen peroxide by rabbit articular chondrocytes. Enhancement by cytokines.
AuthorsTiku ML, Liesch JB, Robertson FM
JournalJ Immunol
PubMed ID2114447
'Recent evidence suggests that reactive oxygen intermediates may play a role in the etiology of cartilage matrix degradation in arthritis. We have previously established that normal articular chondrocytes can functionally act as macrophages. These functions include expression of class II MHC Ag, presentation of Ag and induction of mixed and ... More
Diacylglycerols modulate human polymorphonuclear neutrophil responsiveness: effects on intracellular calcium mobilization, granule exocytosis, and superoxide anion production.
AuthorsSmith RJ, Sam LM, Justen JM
JournalJ Leukoc Biol
PubMed ID2836539
'The synthetic diacylglycerols (DG), sn-1,2-dihexanoylglycerol (diC6), sn-1,2-dioctanoylglycerol (diC8), and 1-oleoyl-2-acetylglycerol (OAG) stimulated the release of granule constituents from and superoxide anion (O2-) generation by human polymorphonuclear neutrophils (PMN). The DGs did not induce a rise in the cytosolic-free calcium concentration ([Ca2+]i), as monitored by the fluorescence of PMNs loaded with ... More
Caldesmon inhibits nonmuscle cell contractility and interferes with the formation of focal adhesions.
AuthorsHelfman DM, Levy ET, Berthier C, Shtutman M, Riveline D, Grosheva I, Lachish-Zalait A, Elbaum M, Bershadsky AD
JournalMol Biol Cell
PubMed ID10512853
'Caldesmon is known to inhibit the ATPase activity of actomyosin in a Ca(2+)-calmodulin-regulated manner. Although a nonmuscle isoform of caldesmon is widely expressed, its functional role has not yet been elucidated. We studied the effects of nonmuscle caldesmon on cellular contractility, actin cytoskeletal organization, and the formation of focal adhesions ... More
Inhibitory effect of 8-bromo cyclic GMP on an extracellular Ca2+-dependent arachidonic acid liberation in collagen-stimulated rabbit platelets.
AuthorsMatsuoka I, Nakahata N, Nakanishi H
JournalBiochem Pharmacol
PubMed ID2544181
'The inhibitory effect of cyclic GMP on collagen-induced platelet activation was studied using 8-bromo cyclic GMP (8brcGMP) in washed rabbit platelets. Addition of collagen (1 micrograms/ml) to platelet suspension caused shape change and aggregation associated with thromboxane (TX) A2 formation. 8brcGMP (10-1000 microM) inhibited collagen-induced platelet aggregation and TXA2 formation ... More
Hyaluronic acid increases motility/intracellular CA2+ concentration in human sperm in vitro.
AuthorsBains R, Miles DM, Carson RJ, Adeghe J
JournalArch Androl
PubMed ID11554683
'This study investigated the mechanisms of the stimulatory effect of hyaluronic acid on motility in human sperm in vitro. A method, involving the measurement of forward progression through an agarose gel. was used to measure sperm motility quantitatively. Changes in intracellular Ca2+ concentrations in sperm were detected using the fluorescent ... More
Phorbol esters and oleoyl acetoyl glycerol enhance release of arachidonic acid in platelets stimulated by Ca2+ ionophore A23187.
AuthorsHalenda SP, Zavoico GB, Feinstein MB
JournalJ Biol Chem
PubMed ID3930496
'Washed human platelets prelabeled with [14C]arachidonic acid and then exposed to the Ca2+ ionophore A23187 mobilized [14C]arachidonic acid from phospholipids and formed 14C-labeled thromboxane B2, 12-hydroxy-5-8,10-heptadecatrienoic acid, and 12-hydroxy-5,8,10,14-eicosatetraenoic acid. Addition of phorbol myristate acetate (PMA) by itself at concentrations from 10 to 1000 ng/ml did not release arachidonic acid ... More
Trans to cis proton concentration gradients accelerate ionophore A23187-mediated net fluxes of Ca2+ across the human red cell membrane.
AuthorsVestergaard-Bogind B, Stampe P
JournalBiochim Biophys Acta
PubMed ID6432046
'Ionophore A23187-mediated net influx of Ca2+ in ATP-depleted human red cells was studied as a function of the pH and the proton concentration gradient across the membranes. Utilizing the Ca2+-induced increase in K+ conductance of the cell membranes, various CCCP-mediated proton gradients were raised across the membranes of cells suspended ... More
Cyclic AMP and calcium in mouse mammary tumour virus expression: effects and post-transcriptional site of action.
AuthorsBolander FF, Blackstone ME, Bradham BM
JournalJ Mol Endocrinol
PubMed ID2168707
'The role of cyclic AMP (cAMP), calcium, calmodulin and protein kinase C (PKC) in the expression of both mouse mammary tumour virus (MMTV) RNA and an MMTV glycoprotein, gp58, was investigated in normal mammary epithelium in culture. None of these second messengers had any effect on MMTV RNA. Dibutyryl cAMP ... More
Regulation of neuronal growth cone filopodia by intracellular calcium.
AuthorsRehder V, Kater SB
JournalJ Neurosci
PubMed ID1494951
'Filopodia have been regarded as the sensory extensions of neuronal growth cones. As such, filopodia assay distant environments and are important for directing growth cones toward their targets. Since the territory encountered by a growth cone depends on the area spanned by the filopodia, changes in filopodial length or number ... More
Quantitation of microparticles released from coated-platelets.
AuthorsDale GL, Remenyi G, Friese P,
JournalJ Thromb Haemost
PubMed ID16102115
'Dual agonist stimulation of platelets with thrombin and convulxin results in generation of coated-platelets, a sub-population of cells known formerly as COAT-platelets (collagen and thrombin). Coated-platelets retain several procoagulant proteins on their surface and express phosphatidylserine (PS). In this report, we utilize a new methodology to demonstrate that coated-platelets also ... More
Fluoride binding to the calcium ATPase of sarcoplasmic reticulum converts its transport sites to a low affinity, lumen-facing form.
AuthorsMurphy AJ, Coll RJ
JournalJ Biol Chem
PubMed ID1387398
'The sarcoplasmic reticulum CaATPase forms an inactive complex with fluoride (CaATPase-F), which in the absence of calcium reactivates very slowly (t1/2 approximately 40 h at 25 degrees C). Reactivation is greatly accelerated (greater than 10(3)) by calcium in the millimolar range provided it has access to luminal sites of the ... More
Intracellular free magnesium in excitable cells: its measurement and its biologic significance.
AuthorsAlvarez-Leefmans FJ, Giraldez F, Gamiño SM
JournalCan J Physiol Pharmacol
PubMed ID3113706
'As a necessary cofactor for hundreds of enzymes, intracellular Mg2+ influences a wide range of cellular functions such as transmembrane transport of other ions, glycolysis, respiration, muscle contraction, and phosphorylation of ion channels. Unlike Ca2+, Mg2+ does not seem to have a "trigger" function. However, the wide range of enzymes ... More
Differential lymphocyte growth-modifying effects of oxidants: changes in cytosolic Ca+2.
AuthorsDuncan DD, Lawrence DA
JournalToxicol Appl Pharmacol
PubMed ID2506676
'An increase in the concentration of cytosolic Ca+2 ([Ca-2]i) is among the earliest changes seen in mitogen-stimulated lymphocytes and is a consequence of signal transduction which usually results in the initiation of cell cycle progression. However, increased [Ca+2]i has also been correlated with cytotoxicity. We have determined whether modulations of ... More
Evaluation of mouse sperm acrosomal status and viability by flow cytometry.
AuthorsTao J, Critser ES, Critser JK
JournalMol Reprod Dev
PubMed ID8257567
'A procedure was developed to evaluate mouse sperm acrosomal status and viability simultaneously utilizing flow cytometry. Four fluorescein isothiocyanate (FITC)-conjugated lectins, peanut agglutinin (PNA), concanavalin agglutinin (ConA), Pisum sativum agglutinin (PSA), and soybean agglutinin (SBA), were investigated, with PNA providing the greatest sensitivity and specificity in distinguishing acrosome-present and acrosome-absent ... More
The possible involvement of the phospholipid phase of membranes in mediating the effects of verapamil on Ca2+ transport.
AuthorsErdreich A, Rahamimoff H
JournalBiochem Pharmacol
PubMed ID3107570
'The effect of verapamil in a model system of A23187-induced Ca2+-uptake into liposomes was studied. This was done in order to separate the effects of verapamil on the lipid phase of membranes from its effects on membraneous proteins. In the absence of A23187, the liposomes exhibited a very low Ca2+ ... More
Comparative investigations on the uptake of phallotoxins, bile acids, bovine lactoperoxidase and horseradish peroxidase into rat hepatocytes in suspension and in cell cultures.
AuthorsPetzinger E, Frimmer M
JournalBiochim Biophys Acta
PubMed ID3120787
'Two alternative uptake mechanisms for phallotoxins by liver cells are debated: carrier-mediated uptake and receptor-mediated endocytosis. We have compared the properties of hepatocellular uptake of the phallotoxins, phalloidin and demethylphalloin, with the uptake of cholate as a substrate for carrier-mediated uptake and compared with iodinated bovine lactoperoxidase or iodinated horseradish ... More
Ionophore 4-BrA23187 transports Zn2+ and Mn2+ with high selectivity over Ca2+.
AuthorsErdahl WL, Chapman CJ, Wang E, Taylor RW, Pfeiffer DR
JournalBiochemistry
PubMed ID8901524
'The cation transport selectivities of the Ca2+ ionophores A23187, Ionomycin, and 4-BrA23187 have been determined using a model system comprised of phospholipid vesicles loaded with the chelator/indicator Quin-2. At pH 7.00 and a 100 microM concentration of the cations, A23187 displays the transport selectivity sequence Zn2+ > Mn2+ > Ca2+ ... More
Modulation of calcium flux influences interleukin 1 beta effects on insulin release from isolated islets of Langerhans.
'The controlled flux of calcium across the cell membrane is intimately linked to the release of insulin from pancreatic beta-cells, but the uncontrolled influx of calcium is a common final denominator of cell death. Because interleukin 1 has been shown to be cytotoxic to beta-cells in isolated rat islets of ... More
Influence of the calcium ionophore A23187 on rat egg behavior and cortical F-actin.
AuthorsBattaglia DE, Gaddum-Rosse P
JournalGamete Res
PubMed ID3148541
'Rat eggs treated with the calcium ionophore A23187 and subjected to long-term observation by phase microscopy were found to undergo many developmental changes that are normally associated with fertilization. These included cortical granule exocytosis and the abstriction of the second polar body. In addition, time-lapse video microscopy revealed that, unlike ... More
Dynamics of intracellular free Mg2+ changes in a vascular smooth muscle cell line.
AuthorsQuamme GA, Dai LJ, Rabkin SW
JournalAm J Physiol
PubMed ID8342644
'Intracellular free Mg2+ concentration ([Mg2+]i) has been implicated in the pathogenesis of hypertension. It has been postulated that Mg2+ through its antagonistic effects on intracellular Ca2+ concentration may affect tension and contractility of vascular smooth muscle cells. An established cell line of rat thoracic aorta cells (A10) was cultured on ... More
The characterization and quantification of antigen-induced Ca2+ oscillations in a rat basophilic leukaemia cell line (RBL-2H3).
AuthorsNarenjkar J, Marsh SJ, Assem ES
JournalCell Calcium
PubMed ID10668564
'Using the ratiometric Ca2+ indicator, indo-1, the antigen-induced increase in intracellular Ca2+ concentration ([Ca2+]i) was measured in individual RBL-2H3 cells which had been passively sensitized with monoclonal antibody to the dintrophenyl (DNP) haptenic group. Antigenic stimulation using DNP-human serum albumin conjugate (DNP-HSA) induced concentration-dependent asynchronous Ca2+ oscillations, or irregular spikes. ... More
Fast axonal transport is modulated by altering trans-axolemmal calcium flux.
AuthorsBreuer AC, Bond M, Atkinson MB
JournalCell Calcium
PubMed ID1586941
'Factors involved in fast axonal transport (motor proteins, microtubules, organelles, etc.) have been identified but the molecular mechanism controlling transport is unknown. We used video enhanced microscopy to directly evaluate the effect of calcium on fast axonal transport (FAxT). FAxT alterations included rapid speed decreases (within minutes) in Ca2+ free ... More
Disparate effects of activators of protein kinase C on HL-60 promyelocytic leukemia cell differentiation.
AuthorsMorin MJ, Kreutter D, Rasmussen H, Sartorelli AC
JournalJ Biol Chem
PubMed ID3114251
'In previously published studies (Kreutter, D., Caldwell, A. B., and Morin, M. J. (1985) J. Biol. Chem. 260, 5979-5984), we demonstrated that the activation of the calcium- and phospholipid-dependent protein kinase C by phorbol esters was dissociable from the induction of monocytic differentiation by these agents in HL-60 promyelocytic leukemia ... More
Phospholipid scramblase activation pathways in lymphocytes.
AuthorsWilliamson P, Christie A, Kohlin T, Schlegel RA, Comfurius P, Harmsma M, Zwaal RF, Bevers EM
JournalBiochemistry
PubMed ID11434775
'In erythrocytes and platelets, activation of a nonspecific lipid flipsite termed the scramblase allows rapid, bidirectional transbilayer movement of all types of phospholipids. When applied to lymphoid cells, scramblase assays reveal a similar activity, with scrambling rates intermediate between those seen in platelets and erythrocytes. Scrambling activity initiated in lymphoid ... More
Inhibition of endocytosis from coated pits by acidification of the cytosol.
AuthorsSandvig K, Olsnes S, Petersen OW, van Deurs B
JournalJ Cell Biochem
PubMed ID2893802
'Binding and endocytosis of the ligands transferrin, epidermal growth factor (EGF), and ricin were measured in a number of different cell lines after treatment of cells with compounds that react with SH-groups and under conditions where the cytosolic pH was lowered. N-ethylmalemide and diamide irreversibly inhibited endocytosis of all ligands ... More
Regulation of receptor-mediated protein kinase C membrane trafficking by autophosphorylation.
AuthorsFeng X, Becker KP, Stribling SD, Peters KG, Hannun YA
JournalJ Biol Chem
PubMed ID10828076
'Signal transduction via protein kinase C (PKC) is closely regulated by its subcellular localization. In response to activation of cell-surface receptors, PKC is directed to the plasma membrane by two membrane-targeting domains, namely the C1 and C2 regions. This is followed by the return of the enzyme to the cytoplasm, ... More
A polymorphic protease-activated receptor 2 (PAR2) displaying reduced sensitivity to trypsin and differential responses to PAR agonists.
'Protease-activated receptor 2 (PAR2) is a trypsin-activated member of a family of G-protein-coupled PARs. We have identified a polymorphic form of human PAR2 (PAR(2)F240S) characterized by a phenylalanine to serine mutation at residue 240 within extracellular loop 2, with allelic frequencies of 0.916 (Phe(240)) and 0.084 (Ser(240)) for the wild-type ... More
Regulating the retention of T-cell receptor alpha chain variants within the endoplasmic reticulum: Ca(2+)-dependent association with BiP.
AuthorsSuzuki CK, Bonifacino JS, Lin AY, Davis MM, Klausner RD
JournalJ Cell Biol
PubMed ID1649196
'Immunoglobulin heavy chain binding protein (BiP, GRP 78) coprecipitates with soluble and membrane-associated variants of the T-cell antigen receptor alpha chain (TCR-alpha) which are stably retained within the ER. Chelation of Ca2+ during solubilization of cells leads to the dissociation of BiP from the TCR-alpha variants, which is dependent upon ... More
Ca2+ transport by reconstituted synaptosomal ATPase is associated with H+ countertransport and net charge displacement.
AuthorsSalvador JM, Inesi G, Rigaud JL, Mata AM
JournalJ Biol Chem
PubMed ID9660785
'The synaptosomal plasma membrane Ca2+-ATPase (PMCA) purified from pig brain was reconstituted with liposomes prepared by reverse phase evaporation at a lipid to protein ratio of 150/1 (w/w). ATP-dependent Ca2+ uptake and H+ ejection by the reconstituted proteoliposomes were demonstrated by following light absorption and fluorescence changes undergone by arsenazo ... More
The effects of A23187 on the phospholipid phase transition of large unilamellar vesicles (LUVs) as detected by ultrasound spectroscopy.
AuthorsMa LD, Magin RL, Dunn F
JournalBiochim Biophys Acta
PubMed ID2154261
'The effect of the hydrophobic Ca2+ ionophore, A23187, on the phospholipid dynamics of large unilamellar vesicle (LUVs: 4: 1 (w/w) mixture of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG] membranes, as a function of A23187 content, was investigated using techniques sensitive to the phospholipid phase transition. The ultrasonic absorption per wavelength, alpha ... More
Role of Ca2+ in H+ transport by rabbit gastric glands studied with A23187 and BAPTA, an incorporated Ca2+ chelator.
AuthorsMichelangeli F, Ruiz MC, Fernández E, Ciarrocchi A
JournalBiochim Biophys Acta
PubMed ID2503036
'The role of Ca2+ in stimulation of H+ gastric secretion by cAMP-dependent and -independent secretagogues was studied in isolated rabbit glands using Ca2+ ionophore, A23187, and an intracellular Ca2+ chelator (BAPTA, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid) incorporated as its acetoxymethyl ester (BAPTA-AM). Acetylcholine (ACh), tetragastrin (TG), histamine and forskolin induced a transitory increase ... More
Ca2+ ionophore A23187 and thrombin inhibit the pertussis-toxin-induced ADP-ribosylation of the alpha-subunit of the inhibitory guanine-nucleotide-binding protein and other proteins in human platelets.
AuthorsSiess W, Wilhelm B, Aepfelbacher M, Gennity JM
JournalEur J Biochem
PubMed ID1935970
'Inhibitory guanine-nucleotide-binding proteins (Gi proteins) are substrates for pertussis toxin and the decreased pertussis-toxin-dependent ADP ribosylation of Gi proteins upon prior specific hormonal stimulation of cells is thought to reflect the receptor-mediated activation of Gi proteins, leading to their subsequent dissociation into alpha i and beta/gamma subunits. In the present ... More
Targeting Janus kinase 3 in mast cells prevents immediate hypersensitivity reactions and anaphylaxis.
AuthorsMalaviya R, Zhu D, Dibirdik I, Uckun FM
JournalJ Biol Chem
PubMed ID10480916
'Janus kinase 3 (JAK3), a member of the Janus family protein-tyrosine kinases, is expressed in mast cells, and its enzymatic activity is enhanced by IgE receptor/FcepsilonRI cross-linking. Selective inhibition of JAK3 in mast cells with 4-(4''-hydroxylphenyl)-amino-6, 7-dimethoxyquinazoline) (WHI-P131) blocked the phospholipase C activation, calcium mobilization, and activation of microtubule-associated protein ... More
Intracellular free zinc and zinc buffering in human red blood cells.
AuthorsSimons TJ
JournalJ Membr Biol
PubMed ID1774775
'Zn2+ has been allowed to equilibrate across the red cell membrane using two agents that increase membrane permeability to this ion: the ionophore A23187 and the specific carrier ethylmaltol. Extracellular free Zn2+ was controlled with EGTA (1,2-di(2-aminoethoxy)ethane-NNN''N''tetra-acetic acid] buffers, except in the case of ethylmaltol, which itself acts as a ... More
CD28 utilizes Vav-1 to enhance TCR-proximal signaling and NF-AT activation.
AuthorsMichel F, Mangino G, Attal-Bonnefoy G, Tuosto L, Alcover A, Roumier A, Olive D, Acuto O
JournalJ Immunol
PubMed ID11034388
'The mechanism through which CD28 costimulation potentiates TCR-driven gene expression is still not clearly defined. Vav-1, an exchange factor for Rho GTPases thought to regulate, mainly through Rac-1, various signaling components leading to cytokine gene expression, is tyrosine phosphorylated upon CD28 engagement. Here, we provide evidence for a key role ... More
Intracellular Ca2+ and cytotoxicity.
AuthorsLynn WS, Mathews D, Cloyd M, Wallwork JC, Thompson A, Sachs C
JournalArch Environ Health
PubMed ID2573316
'Following injury or activation in some immune cell lines, elevation of intracellular Ca2+ concentration (Cai2+) is an early and major event that precedes cell death. Agents shown to elevate Cai2+ and to result subsequently in the death of some cells include human immunodeficiency virus (HIV) (in T4+ cells), 25-hydroxy cholesterol, ... More
Synergistic stimulation of luteinizing hormone (LH) release by protein kinase C activators and Ca2+-ionophore.
AuthorsNaor Z, Eli Y
JournalBiochem Biophys Res Commun
PubMed ID3161505
'When cultured pituitary cells were stimulated with synthetic diacylglycerol such as 1-oleoyl-2-acetylglycerol (OAG), or with a potent tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA), which are known stimulators of Ca2+-activated, phospholipid-dependent protein kinase (protein kinase C), enhanced release of luteinizing hormone (LH) was observed. Similarly, LH release was also stimulated by the Ca2+-ionophore, ... More
Quantitation of bovine sperm cytoplasmic calcium with Quin-2 and Fura-2: evidence that external calcium does not have direct access to the sperm cytoplasm.
AuthorsVijayaraghavan S, Hoskins D
JournalCell Calcium
PubMed ID2776189
'Internal calcium levels of sperm loaded with Quin-2 in the absence or presence of exogenous calcium were 63 +/- 5 and 189 +/- 19 nM, respectively. These values were similar to those determined by Fura-2. Surprisingly, however, dye loaded sperm depleted of internal calcium did not take up calcium from ... More
Signal transduction in monocytes and granulocytes measured by multiparameter flow cytometry.
AuthorsLund-Johansen F, Olweus J
JournalCytometry
PubMed ID1451600
'The novel calcium indicator fura red and the oxidative burst indicator dihydrorhodamine (both excited at 488 nm) were used in combination with multiparameter flow cytometry to allow simultaneous kinetic measurements of calcium fluxes and oxidative bursts in monocytes and granulocytes. Using this method it was possible to obtain direct evidence ... More
Role of calcium in thromboxane B2-mediated injury to rabbit gastric mucosal cells.
AuthorsWong HM, Soper BD, Tepperman BL
JournalDig Dis Sci
PubMed ID7555460
'A sustained increase in cytosolic Ca2+ can damage gastric mucosal cells. The present study has examined the role of Ca2+ in thromboxane B2 (TXB2)-mediated damage of rabbit isolated gastric mucosal cells. Cells were isolated from rabbit oxyntic mucosa by collagenase-EDTA digestion. Cell metabolic activity and cell damage were estimated by ... More
Role of phosphorylation sites and the C2 domain in regulation of cytosolic phospholipase A2.
AuthorsGijon MA, Spencer DM, Kaiser AL, Leslie CC
JournalJ Cell Biol
PubMed ID10366595
'Cytosolic phospholipase A2 (cPLA2) mediates agonist-induced arachidonic acid release, the first step in eicosanoid production. cPLA2 is regulated by phosphorylation and by calcium, which binds to a C2 domain and induces its translocation to membrane. The functional roles of phosphorylation sites and the C2 domain of cPLA2 were investigated. In ... More
BSF-1 action on resting B cells does not require elevation of inositol phospholipid metabolism or increased [Ca2+]i.
AuthorsMizuguchi J, Beaven MA, Ohara J, Paul WE
JournalJ Immunol
PubMed ID3093568
'B cell stimulatory factor-1 (BSF-1) acts on resting B cells to increase expression of class II major histocompatibility complex (MHC) molecules and to prepare for more prompt entry into S phase in response to anti-IgM and lipopolysaccharide. It also acts as a costimulant, with low concentrations of anti-IgM, to cause ... More
Lipopolysaccharide-mediated macrophage activation: the role of calcium in the generation of tumoricidal activity.
AuthorsDrysdale BE, Yapundich RA, Shin ML, Shin HS
JournalJ Immunol
PubMed ID3100622
'As we have reported, calcium ionophore A23187 activates macrophages for tumor cell killing, and the activated macrophages produced a soluble cytotoxic factor (M phi-CF) that is similar, if not identical, to tumor necrosis factor. Based on these observations, we have investigated whether calcium is involved in the activation mediated by ... More
Diacylglycerol causes Ca release from the platelet dense tubular system: comparisons with Ca release caused by inositol 1,4,5-triphosphate.
AuthorsBrass LF, Laposata M
JournalBiochem Biophys Res Commun
PubMed ID3493001
'Platelet activation is often associated with an increase in the cytosolic free Ca concentration that is due in part to Ca release from the dense tubular system. The present studies examine whether the diacylglycerol formed by phosphoinositide hydrolysis during platelet activation contributes to this process. The effect of diacylglycerol on ... More
Proton fluxes associated with the Ca pump in human red blood cells.
AuthorsMilanick MA
JournalAm J Physiol
PubMed ID2156439
'Ca fluxes and H fluxes were measured in human red blood cells at 37 degrees C to characterize the effects of extracellular protons (Hout) on the Ca pump and to determine the stoichiometry of Ca-H exchange. A pH-stat technique was used to measure the rate of H influx, and 45Ca ... More
Cholinergic desensitization of pepsinogen secretion and calcium mobilization of dispersed guinea pig chief cells.
AuthorsCherner JA, Naik L, Singh G
JournalJ Cell Physiol
PubMed ID2298823
'When dispersed chief cells from guinea pig stomach were first incubated with carbachol, washed, and then reincubated with carbachol in fresh incubation solution, the stimulation of pepsinogen secretion and the rise in intracellular calcium concentration during the second incubation were reduced. Carbachol did not cause residual enzyme secretion, but the ... More
Mechanism and specificity of lanthanide series cation transport by ionophores A23187, 4-BrA23187, and ionomycin.
AuthorsWang E, Taylor RW, Pfeiffer DR
JournalBiophys J
PubMed ID9726927
'A23187, 4-BrA23187, and ionomycin transport several lanthanide series trivalent cations at efficiencies similar to Ca2+, when compared at cation concentrations of approximately 10(-5) M, ionophore concentrations of approximately 10(-6) M, and a pH of 7.00. Selectivity sequences and the range of relative rates are as follows: A23187, Nd3+ > La3+ ... More
Chymotryptic-type protease inhibitors block the increase in Ca2+ and Il-2 production in activated Jurkat T cells.
AuthorsAuberger P, Mary D, Breittmayer JP, Aussel C, Fehlmann M
JournalJ Immunol
PubMed ID2521661
'Several chymotryptic-type protease inhibitors were found to inhibit both anti-CD3 mAb- and PHA-induced rise in Ca2+ and IL-2 production in Jurkat T cells. The magnitude of inhibition was a function of the effectors used to stimulate Ca2+ entry and depended on the concentration of the inhibitors. Neither tryptic-type protease inhibitors ... More
The initial phase of gastrulation in sea urchins is accompanied by the formation of bottle cells.
AuthorsNakajima Y, Burke RD
JournalDev Biol
PubMed ID8903358
'The morphogenetic processes responsible for the initial phase of gastrulation in sea urchins have yet to be satisfactorily defined. Using conventional and confocal microscopy we have analyzed the buckling of the vegetal plate to form the archenteron in embryos of Strongylocentrotus purpuratus. The cells of the vegetal plate elongate and ... More
High responsiveness of cytosolic free calcium concentration to angiotensin II in cultured pulmonary arterial myocytes from pulmonary hypertensive rats.
AuthorsLi Z, Zhang Q, Zhao S, Wei M, Cong H, Ouda H, Odajima K, Takemura H
JournalRes Commun Mol Pathol Pharmacol
PubMed ID9344225
'Pulmonary arterial myocytes were cultured from normotensive and pulmonary hypertensive rats. Microfluorimetry of Ca2+ signals in fluo-3-loaded single myocytes at day 7 of culture was performed by a laser-scanned confocal imaging system. The resting level of cytoplasmic Ca2+ concentration ([Ca2+]i) in vascular myocytes obtained from hypertensive rats was higher than ... More
Dynamic continuity of cytoplasmic and membrane compartments between plant cells.
AuthorsBaron-Epel O, Hernandez D, Jiang LW, Meiners S, Schindler M
JournalJ Cell Biol
PubMed ID3346323
'Fluorescence photobleaching was employed to examine the intercellular movement of fluorescein and carboxyfluorescein between contiguous soybean root cells (SB-1 cell line) growing in tissue culture. Results of these experiments demonstrated movement of these fluorescent probes between cytoplasmic (symplastic) compartments. This symplastic transport was inhibited with Ca2+ in the presence of ... More
Effects of quinine and apamin on the calcium-dependent potassium permeability of mammalian hepatocytes and red cells.
AuthorsBurgess GM, Claret M, Jenkinson DH
JournalJ Physiol
PubMed ID6273550
'1. K-sensitive electrodes placed in the extracellular fluid have been used to show that ATP and noradrenaline cause a rapid loss of up to 10% of the K content of isolated guinea-pig hepatocytes. 2. The hypothesis tha this response is a consequence of a rise in the K permeability of ... More
Increase in cytosolic calcium upregulates the synthesis of type 1 plasminogen activator inhibitor in the human histiocytic cell line U937.
AuthorsPeiretti F, Fossat C, Anfosso F, Alessi MC, Henry M, Juhan-Vague I, Nalbone G
JournalBlood
PubMed ID8547638
'In the U937 histiocytic cell line, we investigated the effect of calcium-mobilizing agents with or without tumor necrosis factor-alpha (TNF) on the regulation of the synthesis of plasminogen activator inhibitor-type 1 (PAI-1). Cultured U937 cells were stimulated with ionophore A23187 and thapsigargin with or without TNF. The response was analyzed ... More
Adenosine reduces the Ca2+ transients of isoproterenol-stimulated rat ventricular myocytes.
AuthorsFenton RA, Moore ED, Fay FS, Dobson JG
JournalAm J Physiol
PubMed ID1767814
'Adenosine in the heart attenuates the contractile and metabolic effects of beta-adrenergic stimulation. The effect of adenosine on changes in intracellular Ca2+ concentration [( Ca2+]i) elicited with electrical stimulation was studied in rat ventricular myocytes in the absence and presence of isoproterenol (ISO). Fura-2 was utilized as a Ca2+ indicator. ... More
Effect of an ectokinase inhibitor, K252b, on degranulation and Ca2+ signals of RBL-2H3 cells and human basophils.
AuthorsTeshima R, Saito Y, Ikebuchi H, Rajiva De Silva N, Morita Y, Nakanishi M, Sawada J, Kitani S
JournalJ Immunol
PubMed ID9218617
'We examined the effects of K252b, an ectoprotein kinase inhibitor of microbial origin, on the activation process of RBL-2H3 cells by cross-linking of IgE receptors by the endoplasmic reticulum Ca2+-ATPase inhibitor 2,5-di(tert-butyl)-1,4-hydroquinone or by the Ca2+ ionophore A23187. Analysis of phosphorylation of ectoproteins following IgE receptor cross-linking revealed that K252b ... More
Receptor-mediated phagocytosis by macrophages induces a calcium-dependent transient increase in c-fos transcription.
'The transcription of the c-fos gene and the level of c-fos mRNA in mouse peritoneal macrophages are rapidly, strongly and transiently increased after Fc- and C3b-mediated phagocytosis, but not after phagocytosis of latex particles. In order to induce both phagocytosis and a rise in c-fos mRNA, binding to receptors must ... More
Parathyroid hormone promotes the disassembly of cytoskeletal actin and myosin in cultured osteoblastic cells: mediation by cyclic AMP.
AuthorsEgan JJ, Gronowicz G, Rodan GA
JournalJ Cell Biochem
PubMed ID1848561
'Parathyroid hormone (PTH) alters the shape of osteoblastic cells both in vivo and in vitro. In this study, we examined the effect of PTH on cytoskeletal actin and myosin, estimated by polyacrylamide gel electrophoresis of Triton X-100 (1%) nonextractable proteins. After 2-5 minutes, PTH caused a rapid and transient decrease ... More
Presence of Na+/Ca2+ exchange activity and its role in regulation of intracellular calcium concentration in bovine adrenal chromaffin cells.
AuthorsChern YJ, Chueh SH, Lin YJ, Ho CM, Kao LS
JournalCell Calcium
PubMed ID1633612
'The presence of a Na+/Ca2+ exchanger in bovine adrenal chromaffin cells was demonstrated by measuring the efflux of 45Ca2+ which had been preloaded into cells by a brief depolarization. The efflux of 45Ca2+ was dependent on extracellular Na+ (Na+o); 45Ca2+ efflux was significantly decreased by replacing Na+o with N-methylglucamine (NMG), ... More
Ion diffusion potentials across mycoplasma membranes determined by a novel method using a carbocyanine dye.
AuthorsSchummer U, Schiefer HG
JournalArch Biochem Biophys
PubMed ID3947080
'The influence of transmembrane ion fluxes on mycoplasma membrane potentials was studied. Electric membrane potential was calibrated vs fluorescence intensity of a potential-sensitive carbocyanine dye according to delta psi = (RT/F) X log([aIN(1 - IN) - b]/Kint), where IN = I/I0, I0 = maximal fluorescence intensity (obtained for delta psi----infinity), ... More
Caspase independent/dependent regulation of K(+), cell shrinkage, and mitochondrial membrane potential during lymphocyte apoptosis.
AuthorsBortner CD, Cidlowski JA
JournalJ Biol Chem
PubMed ID10419518
'The loss of cell volume is a fundamental feature of apoptosis. We have previously shown that DNA degradation and caspase activity occur only in cells which have shrunken as a result of potassium and sodium efflux (Bortner, C. D., Hughes, F. M., Jr., and Cidlowski, J. A. (1997) J. Biol. ... More
Tumor necrosis factor-alpha stimulates prolactin release from anterior pituitary cells: a possible involvement of intracellular calcium mobilization.
AuthorsKoike K, Masumoto N, Kasahara K, Yamaguchi M, Tasaka K, Hirota K, Miyake A, Tanizawa O
JournalEndocrinology
PubMed ID2036963
'We investigated the effects of tumor necrosis factor-alpha (TNF alpha), a cytokine produced as one aspect of inflammatory reactions, on the intracellular free calcium concentration in single pituitary cells using a calcium-sensitive fluorescent dye, indo-1, and a digital imaging fluorescence microscopic system. TNF alpha induced an increase in intracellular free ... More
Increased aggregation and secretion responses of human platelets when loaded with the calcium fluorescent probes quin2 and fura-2.
AuthorsLanza F, Beretz A, Kubina M, Cazenave JP
JournalThromb Haemost
PubMed ID3672425
'Incorporation into human platelets of the calcium fluorescent indicators quin2 or fura-2 at low concentrations used to measure intracellular free calcium leads to the potentiation of the effects of agonists on platelets. This was shown by increased aggregatory and secretory responses of quin2 or fura-2 loaded platelets after stimulation with ... More
Depolarization of rat basophilic leukemia cells inhibits calcium uptake and exocytosis.
AuthorsMohr FC, Fewtrell C
JournalJ Cell Biol
PubMed ID2950123
'We have investigated the unusual observation that depolarization of rat basophilic leukemia cells in high potassium not only fails to induce secretion, but also inhibits the secretion induced when receptors for IgE are aggregated by antigen. Antigen-stimulated 45Ca uptake and the rise in cytoplasmic free ionized calcium measured with the ... More
Role of enhanced inositol phospholipid metabolism in neutrophil activation.
AuthorsTakenawa T, Ishitoya J, Homma Y, Kato M, Nagai Y
JournalBiochem Pharmacol
PubMed ID2988563
'When guinea pig neutrophils were stimulated with chemotactic peptide [formylmethionyl-leucyl-phenylalanine (fMLP)], a marked release of lysosomal enzyme and production of superoxide anion were detected. The breakdown of phosphatidylinositol 4,5-bisphosphate (TPI) and the subsequent formation of diacylglycerol, phosphatidic acid and free arachidonic acid also occurred during the processes. Ca2+ ionophore A23187 ... More