Primary Mouse Hippocampal Neurons - FAQs

查看更多产品信息 Primary Mouse Hippocampal Neurons - FAQs (A15587)

3 个常见问题解答

How were the Primary Mouse Hippocampal Neurons isolated? How specific was the dissection area (does it include the dentate gyrus, CA1, CA2, and/or CA3)? How much glutamate should I use for culturing them?

The neurons isolated are likely from all the areas of the hippocampus you listed. The glutamate is used to establish the culture. If the media is being changed every day, the media should have glutamate supplemented through day 4 after recovery. Beyond day 4, it is not necessary and likely not preferred. Any glutamate can be used as long as it is of a higher purity such as FCC, USP, ACS, etc. grade.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.