重组人层粘连蛋白-521
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重组人层粘连蛋白-521
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重组人层粘连蛋白-521

重组人层黏连蛋白 521 (rhLaminin-521) 是一种重组人蛋白，用于为人多能干细胞 (PSC) 的无饲养层培养提供一层成分明确的表面。业已证实，配合 Essential 8™ 培养基一起使用时，即使没有小分子抑制剂，rhLaminin-521了解更多信息
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货号数量
A29248100 μg
A292491 mg
货号 A29248
价格（CNY)
2,180.25
飞享价
Ends: 31-Dec-2025
2,601.00
共减 420.75 (16%)
Each
添加至购物车
数量:
100 μg
价格（CNY)
2,180.25
飞享价
Ends: 31-Dec-2025
2,601.00
共减 420.75 (16%)
Each
添加至购物车
重组人层黏连蛋白 521 (rhLaminin-521) 是一种重组人蛋白，用于为人多能干细胞 (PSC) 的无饲养层培养提供一层成分明确的表面。业已证实，配合 Essential 8™ 培养基一起使用时，即使没有小分子抑制剂，rhLaminin-521 也可促进应激条件下的细胞生存。研究表明，rhLaminin-521 能够支持 PSC 生长达 >20 代而没有任何核型异常迹象，并保持 PSC 分化为所有三种生殖细胞系的能力。与 Essential 8 培养基联合使用时，rhLaminin-521 还可在饲养层依赖性培养到无饲养层培养过渡期间为人体细胞提供理想的重编程效率以及为人多能干细胞提供理想的存活率。有关结合培养基和底物的试剂盒，请参阅 Essential 8™ 适应试剂盒。

rhLaminin-521 具有下列优势：
•稳健 — 仅需极少的工作量，即可实现细胞在饲养层依赖性培养系统与无饲养层培养系统之间的过渡
• 高效 — 轻松将人成纤维细胞重编程为多能干细胞
• 生理相关 — 可在尽可能减少分化的情况下扩增细胞
• 可扩展 — 作为单细胞对干细胞系进行传代，不影响遗传稳定性

无故障过渡
配合 Essential 8 培养基一起使用时，rhLaminin-521 可提供稳健的培养基质，在从饲养层依赖性培养系统过渡期间支持多能干细胞的粘附和扩增。

高效的重编程
rhLaminin-521 已被证明能够增加人成纤维细胞重编程过程中获得的集落数量，而这对于构建体外疾病模型非常重要。

可靠的扩增
rhLaminin-521 在囊胚中表达，因此提供了一种模拟人干细胞生态位的环境。

易于扩大规模
rhLaminin-521 可在没有 ROCK 抑制剂的情况下促进多能干细胞作为单细胞的稳定维持。
仅供科研使用。不可用于诊断程序。
规格
细胞类型干细胞
培养类型干细胞（人、iPS - 诱导多能干细胞、胚胎）
纯度或质量等级hESC 合格
数量100 μg
有效期24 个月
运输条件干冰
来源无异种成分
分类无异种成分
产品类型细胞外基质
pH6 - 8
Unit SizeEach
内容与储存
在冷冻冰箱（-5 至 -20°C）中避光储存。

常见问题解答 (FAQ)

What is the alternative for rhLaminin-521 (Cat. No. A29249)?

The appropriate alternative for rhLaminin-521 (Cat. No. A29249) would be either Laminin Mouse Protein, Natural (Cat. No. 23017015) or Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A14700).

Find additional tips, troubleshooting help, and resources within our Stem Cell Culture Support Center.

Can I use the Poly-D-Lysine matrix with other coatings?

iPSC-derived neurons have been cultured successfully on Poly-D-Lysine with a secondary coating of mouse and human laminin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for blood cells?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A14700), Geltrex (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for human fibroblasts?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A14700), Geltrex matrix (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use Geltrex matrix instead of rhLaminin-521 for clonal expansion workflows where cells are seeded at 1-5 cells per well of a 96-well plate?

We have not yet evaluated the Geltrex matrix system for clonal expansion in the presence of StemFlex Medium. However, rhLaminin-521 does provide optimal survival of cells following single-cell passaging and thus this matrix is recommended for such critical applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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