IonCode™ 条形码接头 1-384 试剂盒
IonCode™ 条形码接头 1-384 试剂盒
Ion Torrent™

IonCode™ 条形码接头 1-384 试剂盒

IonCode 条形码接头 1-384 试剂盒可提供一套 384 独特条形码接头,专门设计用于与 Ion GeneStudio S5、Ion了解更多信息
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货号数量
A297513840 Reactions
货号 A29751
价格(CNY)
-
数量:
3840 Reactions
IonCode 条形码接头 1-384 试剂盒可提供一套 384 独特条形码接头,专门设计用于与 Ion GeneStudio S5、Ion PGM 和 Ion Proton 半导体测序仪配合使用以实现理想性能。当与 Ion 条形码接头 385-768 和 Ion AmpliSeq 文库试剂 PLUS 或 Ion AmpliSeq 文库试剂盒 2.0 组合使用时,用户可通过该试剂盒混合至 768 扩增子文库,然后进行多重测序分析,从而简化了 Ion 半导体测序工作流程,适合多种应用,包括靶向富集。

主要产品特点:

•使用强大的分子条形码,可在单个测序芯片上对大量扩增子文库样品进行同时检测
•经序列和流式优化,可在池中平均显示所有条形码,同时进行更经济的多重测序运行
•通过设计内置的稳健误差校正功能增加了样品鉴别的可信度

通过条形码接头进行多重分析可实现更高通量
对条形码编码文库进行多重分析可分析多达 768 份样品/运行,从而带来更高的成本效益,并显著降低 PCR、富集和处理的成本和处理要求。对各条形码单独检测以使呈现偏倚降至较低。

进行序列和流式优化以提高性能和效率
条形码接头包含经过优化的序列,以在池中平均显示所有条形码,需要较低的流量即可进行查询,因此获得更经济的多重测序运行。

通过设计内置的稳健误差校正
内置误差校正可以消除错误的样品分配的可能性.
仅供科研使用。不可用于诊断程序。
规格
适用于(应用)动物疾病的检测和鉴别
适用于(设备)Ion GeneStudio S5 系统、Ion PGM™ 系统、Ion Proton™ 系统、Ion Chef™ 系统、Ion OneTouch™ 系统
扩增子文库
反应次数3840
产品线IonCode™
产品类型条形码接头试剂盒
数量3840 Reactions
样品类型DNA
运输条件干冰
工作流程步骤文库条形码
产品规格试剂盒
测序类型基因组和 DNA 测序
Unit SizeEach
内容与储存
4 x 96微孔板,每孔 20 μL

在冰箱(-5 至 -30°C)中储存。

常见问题解答 (FAQ)

What do I need to do to prep the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

When handling barcode adapters, be careful to avoid cross-contamination by changing gloves frequently and by carefully removing the plate seal.
- Pulse vortex the IonCode Barcode Adapters plate several times.
- Centrifuge the IonCode Barcode Adapters plate for 1 minute at 1000 x g to collect contents.
- Carefully remove, then discard the plate seal.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reactions are possible with the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The plate consists of 960 reactions in total with 10 reactions for each of the 96 barcodes.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

In what format are the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The barcodes are pre-diluted, ready-to-use. The 96-well plate format allows multichannel pipetting for more efficient work.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the shipping and storage conditions for IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit is shipped on dry ice and we recommend storing at -30 degrees C to -5 degrees C in an upright position.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples from the Oncomine Comprehensive Assay Plus, manual library preparation can I run on the Ion 550 Chip?

We recommend sequencing up to 5 DNA or RNA samples (4 samples and a non-template control (NTC)) on a single Ion 550 Chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.