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View additional product information for IonCode™ Barcode Adapters 1-384 Kit - FAQs (A29751)
11 product FAQs found
When handling barcode adapters, be careful to avoid cross-contamination by changing gloves frequently and by carefully removing the plate seal.
- Pulse vortex the IonCode Barcode Adapters plate several times.
- Centrifuge the IonCode Barcode Adapters plate for 1 minute at 1000 x g to collect contents.
- Carefully remove, then discard the plate seal.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The plate consists of 960 reactions in total with 10 reactions for each of the 96 barcodes.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The barcodes are pre-diluted, ready-to-use. The 96-well plate format allows multichannel pipetting for more efficient work.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
The IonCode Barcode Adapters 1-96 Kit is shipped on dry ice and we recommend storing at -30 degrees C to -5 degrees C in an upright position.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
We recommend sequencing up to 5 DNA or RNA samples (4 samples and a non-template control (NTC)) on a single Ion 550 Chip.
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The Oncomine Comprehensive Assay Plus, manual library preparation (Cat. No. A46721) is designed to prepare barcoded sample libraries from DNA and RNA. The kit consists of Oncomine Comprehensive Assay Plus DNA (2-pool) (Part No. A45615), RNA Oncomine Comprehensive Assay v3M (2-pool) (Part No. A33637), and two kits of Ion AmpliSeq Library Kit Plus (Cat. No. 4488990). Sufficient reagents are provided to prepare libraries from 24 samples.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
10 ng of nucleic acid input per pool (20 ng of DNA and 20 ng of RNA) isolated from FFPE samples, including fine needle biopsies, is needed for the Oncomine Comprehensive Assay Plus, manual library preparation.
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The assay is optimized for use with formalin-fixed paraffin embedded (FFPE) samples.
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The Oncomine Comprehensive Assay Plus, manual library preparation (Cat. No. A46721) is a targeted, next-generation sequencing (NGS) assay that enables the detection of single nucleotide variants (SNVs), insertions and deletions (INDELs), copy number variations (CNVs), gene fusions, splice variants, tumor mutational burden (TMB), and microsatellite instability (MSI) across 500+ unique genes. This version of the assay is designed for manual library preparation, not for automated library preparation using the Ion Chef System.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
8 samples can be processed per IonCode plate. The kit contains 4 IonCode plates, hence, a total of 32 samples can be processed using the Oncomine Focus Assay, Chef-Ready Library kit.
The sequences for the barcodes can be found in the Barcodes section of your Torrent Browser. You can also download a .csv file containing the full list of sequences in that set.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.