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View additional product information for Phalloidin Labeling Probes - FAQs (R415, A12379, A30104, B7474, A22282, A30105, A22281, A30106, A30107, A22286, P3457, F432, A22285, A22284, A22283, T7471, A22287, B3475, A34055, A12380, O7466, A12381, A34054)
6 product FAQs found
当细胞和组织经由二甲苯或丙酮之类的溶剂处理时（例如组织切片脱石蜡期间），它通过阻止鬼笔环肽结合的方式来影响F- actin。冰冻切片通常不使用有机溶剂洗涤，可以用来代替石蜡切片，也可以使用抗肌动蛋白抗体。
We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.
For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.
Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
If the F-actin is depolymerized, phalloidin will not bind. Any processing steps or any treatments/compound that can promote depolymerization would impact phalloidin binding. Also, any compounds that also bind to F-actin (without depolymerization) may affect phalloidin binding.
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No. They differ only in the dyes attached to the phalloidin. The phalloidin has the same structure in all of the products.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
No. They differ only in the dyes attached to the phalloidin. The phalloidin has the same structure in all of the products.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.