Automated Thermal Cycler (ATC), 96-well, 3-m cable, 1 each - FAQs

View additional product information for Automated Thermal Cycler (ATC), 96-well - FAQs (A31489, A31490, A31491)

6 product FAQs found

Do you offer a thermal cycler that will accommodate fully skirted plates?

Among the thermal cyclers we offer, the only one that will accommodate fully skirted plates is the Automated Thermal Cycler (Cat. No. A31489, A31490, A31491).

Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.

Do any of your thermal cyclers, other than the Automated Thermal Cycler, hold a full skirted PCR plate?

For full skirt plate, a raised heating block is required. None of our thermal cyclers have a raised heating block except the Automated Thermal Cycler.

Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.

Can I change out the block on my Automated Thermal Cycler (Cat. No. A31490)?

Yes, you can change out the ATC block.

For connecting the ATC block, please see the Automated Thermal Cycler System User Guide, page 15.

Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.

How can I monitor my thermal cycler remotely without being on the Cloud?

The Thermal Cycler Fleet Control Software allows monitoring of the following instruments from within a firewall:
SimpliAmp Thermal Cycler
ProFlex PCR System
Veriti Thermal Cycler
VeritiPro Thermal Cycler
Automated Thermal Cycler (ATC)

Which thermocyclers can be controlled using the Thermal Cycler Fleet Control Software?

The Thermal Cycler Fleet Control Software is compatible with the following instruments:
- SimpliAmp Thermal Cycler
- ProFlex PCR System
- Veriti Thermal Cycler
- VeritiPro Thermal Cycler
- Automated Thermal Cycler (ATC)
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler