Pierce™ 磷酸酶抑制剂 Mini 片剂
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Pierce™ 磷酸酶抑制剂 Mini 片剂
Thermo Scientific™

Pierce™ 磷酸酶抑制剂 Mini 片剂

Thermo Scientific Pierce 磷酸酶抑制剂 Mini 片剂可以完全保护细胞裂解物和生物样品,使其免受广谱丝氨酸和苏氨酸磷酸酶的去磷酸化。Pierce 磷酸酶抑制剂 Mini 片剂的特点:•经济实惠—比其他市售配方更节约成本•了解更多信息
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货号数量
A3295720 片
货号 A32957
价格(CNY)
1,453.00
飞享价
Ends: 31-Dec-2025
2,255.00
共减 802.00 (36%)
Each
添加至购物车
数量:
20 片
价格(CNY)
1,453.00
飞享价
Ends: 31-Dec-2025
2,255.00
共减 802.00 (36%)
Each
添加至购物车
Thermo Scientific Pierce 磷酸酶抑制剂 Mini 片剂可以完全保护细胞裂解物和生物样品,使其免受广谱丝氨酸和苏氨酸磷酸酶的去磷酸化。

Pierce 磷酸酶抑制剂 Mini 片剂的特点:
经济实惠—比其他市售配方更节约成本
配方改进—片剂快速溶解成澄清溶液
全面—片剂可抑制酸、碱和蛋白磷酸酶
兼容—与国产或市售细胞裂解试剂和 Thermo Scientific 蛋白检测配合使用

Pierce 磷酸酶抑制剂 Mini 片剂可以保护磷蛋白不被丝氨酸/苏氨酸磷酸酶和酪氨酸磷酸酶去磷酸化。每种磷酸酶抑制剂片剂都含有氟化钠、原钒酸钠、焦磷酸钠和 β-磷酸甘油,可用于广谱抑制磷酸酶活性。片剂在 4°C 下可保持稳定 12 个月,每片剂足以处理 10 mL 溶液。Pierce 磷酸酶抑制剂片剂适合与任何细胞或组织提取物配合使用,以保留下游应用的蛋白磷酸化。

蛋白磷酸化和去磷酸化状态通常用作细胞内生物学通路中关键调节子的分子开/关开关,包括信号转导、细胞分裂和细胞凋亡。为了有效地研究磷酸化信号转导事件,必须将内源性磷酸酶灭活以防止细胞裂解后其不受控制的活性。Pierce 磷酸酶抑制剂片剂可通过显著降低内源性酸、碱和蛋白磷酸酶活性来保留磷蛋白修饰。

Pierce 磷酸酶抑制剂 Mini 片剂兼容大多数基于去污剂的细胞裂解试剂,包括 Pierce 细胞裂解液。如果发生不兼容情况,则在进行这些操作前,可通过透析或脱盐来去除抑制剂组分。

要使用 Pierce 磷酸酶抑制剂 Mini 片剂,只需将 1 片溶解在 10 mL 缓冲液或裂解物中。Pierce 磷酸酶抑制剂 Mini 片剂不含蛋白酶抑制剂。如有必要,蛋白酶抑制剂(如 Pierce 蛋白酶抑制剂片剂)可与磷酸酶抑制剂片剂一起添加。

相关产品
Pierce 蛋白酶抑制剂片剂
Pierce 蛋白酶抑制剂 Mini 片剂
Pierce 蛋白酶和磷酸酶抑制剂 Mini 片剂

仅供科研使用。不可用于诊断程序。
规格
组成氟化钠、正钒酸钠、焦磷酸钠、β-甘油磷酸盐
抑制剂磷酸酶抑制剂
数量20 片
运输条件湿冰
足够用于10 mL 溶液
形式实心
产品线Pierce™
产品类型抑制剂
Unit SizeEach
内容与储存
1 小瓶,在 2°C 至 8°C 下储存

常见问题解答 (FAQ)

After dissolving the Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957), for how long would the solution be stable at 4 °C?

We have no data on long-term stability of the inhibitors at 4°C in aqueous solution. We generally recommend dissolving the tablets just before use.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Would it be possible to dissolve the Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957) in less than 10 mL in order to have a solution of higer concentration?

As it is recommended to use a final inhibitor concentration of 2X - 3X for samples with a high phosphatase activity, it is indeed possible to prepare a solution at these concentrations. However, we would not recommend to go much higher than 3X.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

How should I dissolve Pierce Phosphatase Inhibitor Mini Tablets?

The Pierce Phosphatase Inhibitor Mini Tablets can be dissolved in the lysis reagent that is being used for protein extraction. We recommend dissolving each tablet in 10 mL of the lysis reagent to get a 1X final concentration. To dissolve completely, the solution may be vortexed or placed on a magnetic stir plate with a stir bar.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What formats of protease inhibitors do you offer?

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.