After dissolving the Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957), for how long would the solution be stable at 4 °C?
We have no data on long-term stability of the inhibitors at 4°C in aqueous solution. We generally recommend dissolving the tablets just before use.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.
Would it be possible to dissolve the Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957) in less than 10 mL in order to have a solution of higer concentration?
As it is recommended to use a final inhibitor concentration of 2X - 3X for samples with a high phosphatase activity, it is indeed possible to prepare a solution at these concentrations. However, we would not recommend to go much higher than 3X.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.
How should I dissolve Pierce Phosphatase Inhibitor Mini Tablets?
The Pierce Phosphatase Inhibitor Mini Tablets can be dissolved in the lysis reagent that is being used for protein extraction. We recommend dissolving each tablet in 10 mL of the lysis reagent to get a 1X final concentration. To dissolve completely, the solution may be vortexed or placed on a magnetic stir plate with a stir bar.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?
We do not recommend splitting the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
What formats of protease inhibitors do you offer?
We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.