Pierce 蛋白酶抑制剂片剂
Pierce 蛋白酶抑制剂片剂
Thermo Scientific™

Pierce 蛋白酶抑制剂片剂

Thermo Scientific Pierce 蛋白酶抑制剂片剂含有可高效防止细胞裂解和蛋白提取实验中蛋白水解降解的广谱蛋白酶抑制剂。蛋白酶抑制剂片剂的特点:•改进的配方—片剂快速溶解成澄清溶液• 经济实惠—相同体积的情况下,比其他市售配方更节约成本• 便利—即用型、完全披露、广谱配方• 兼容—直接与了解更多信息
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货号 A32963
价格(CNY)
3,406.00
Each
添加至购物车
价格(CNY)
3,406.00
Each
添加至购物车
Thermo Scientific Pierce 蛋白酶抑制剂片剂含有可高效防止细胞裂解和蛋白提取实验中蛋白水解降解的广谱蛋白酶抑制剂。

蛋白酶抑制剂片剂的特点:
改进的配方—片剂快速溶解成澄清溶液
经济实惠—相同体积的情况下,比其他市售配方更节约成本
便利—即用型、完全披露、广谱配方
兼容—直接与 Pierce BCA 测定配合使用

Pierce 蛋白酶抑制剂片剂通过抑制细胞裂解物样品中通常存在的丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶及氨基肽酶防止蛋白发生蛋白水解降解。该片剂含有 AEBSF、抑肽酶、苯丁抑制素、E-64、亮肽素和抑胃肽 A。在 4°C 下储存时可保持稳定长达 12 个月,每片片剂足以处理 50 mL 的溶液,它还适用于从组织和培养细胞中提取蛋白之前的试剂制备。

蛋白酶构成一大类酶,包括肽链内切酶和肽链外切酶,它们催化蛋白水解分解为肽或氨基酸。蛋白酶存在于细胞区室内,但细胞裂解后蛋白酶释放,从而导致有价值的蛋白降解。因此,通常需要添加多种已知化合物以灭活或抑制细胞裂解缓冲液或细胞提取物中的蛋白酶。

要使用 Pierce 蛋白酶抑制剂片剂,只需将 1 片片剂溶解于 50 mL 的缓冲液或裂解物。蛋白酶抑制剂配方兼容大多数基于去污剂的细胞裂解试剂,包括 Pierce 细胞裂解液。Pierce 蛋白酶抑制剂片剂不含磷酸酶抑制剂。如有必要,磷酸酶抑制剂(如 Pierce 磷酸酶抑制剂微型片剂)可与蛋白酶抑制剂片剂一起添加。

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Pierce 蛋白酶抑制剂片剂(无 EDTA)
Pierce 蛋白酶抑制剂微型片剂
Pierce 磷酸酶抑制剂微型片剂
Pierce 蛋白酶和磷酸酶抑制剂微型片剂

For Research Use Only. Not for use in diagnostic procedures.
规格
描述Pierce 蛋白酶抑制剂片剂
抑制剂蛋白酶抑制剂
数量20 片
运输条件室温
足够用于50 mL 溶液
形式实心
产品线Pierce™
产品类型抑制剂
Unit SizeEach

常见问题解答 (FAQ)

Do Pierce Protease Inhibitor Tablets (Cat. No. A32963) dissolve quickly?

Pierce Protease Inhibitor Tablets (Cat. No. A32963) dissolve quickly to form a clear solution. We recommend dissolving 1 tablet in 50 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957) can be added along with the protease inhibitor tablets.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What formats of protease inhibitors do you offer?

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer any of the components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets individually?

Yes, the following components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are offered separately:

- AEBSF (Cat. No. 78431)
- Aprotinin (Cat. No. 78432)
- Bestatin (Cat. No. 8433)
- E-64 (Cat. No. 78434)
- Leupeptin (Cat. No. 78435)
- Pepstatin A (Cat. No. 78436)
- EDTA (Cat. No. 17892).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the protease and phosphatase inhibitor tablets and mini tablets?

The final concentration of each component in the protease and phosphatase inhibitor tablets and mini tablets is proprietary. We release only the identities of the inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.