CF1 小鼠胚胎成纤维细胞，经过辐照

Mouse (ICR) Inactivated Embryonic Fibroblasts and CF1 Mouse Embryonic Fibroblasts, irradiated are inactivated feeder layer fibroblast cells that are isolated from different mouse strains. Researchers often have a preference for one or the other. Neither of these can be used to reprogram fibroblasts.

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Either method will work in arresting cell division. However, the irradiation process will ensure that cell division will cease regardless of cell aggregation. Cell clumping can potentially not inactivate all cells when using mitomycin C, as cells within clumps may not be exposed to the mitomycin C. Irradiated cells are preferred by those who have concerns about chemical treatment. Mitomycin C-treated cells are preferred by those who have concerns about DNA damage from irradiation.

CF1 mouse embryonic fibroblasts do not have drug resistance. CF6 mouse embryonic fibroblasts are resistant to Neomycin/geneticin (G418). DR 4 mouse embryonic fibroblasts are resistant to geneticin (G418), puromycin, hygromycin, and 6-thioguanine.

We recommend seeding these cells at densities ranging from 2 x 10E4 to 5.5 x 10E4 cells/cm2. A good starting point is 3 x 10E4 cells/cm2. If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the feeder cells are too dense, the feeder layer may detach from the plate, and the culture will be lost.

These cells should be plated 24 hours prior to plating the ESCs or iPSCs and should be used for only 7-10 days.