High-Select™ 去除离心柱
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High-Select™ 去除离心柱
High-Select™ 去除离心柱
High-Select™ 去除离心柱
Thermo Scientific™

High-Select™ 去除离心柱

Thermo Scientific High Select HSA/免疫球蛋白去除树脂可减少人血浆样品中的高丰度白蛋白和抗体组分,有助于为质谱分析或 1D 或 2D 电泳制备样品。树脂以方便的一次性微型离心柱形式提供,可用于处理了解更多信息
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货号数量产品类型纯化目标
A363696柱色谱柱Top14 Abundant Protein
A363656柱色谱柱HSA/Immunoglobulin
A3636624柱色谱柱HSA/Immunoglobulin
A3636710柱色谱柱HSA/Immunoglobulin
A3636850 mL树脂HSA/Immunoglobulin
A3637110柱色谱柱Top14 Abundant Protein
A3637250 mL树脂Top14 Abundant Protein
A3637024柱色谱柱Top14 Abundant Protein
货号 A36369
价格(CNY)
2,525.00
飞享价
Ends: 31-Dec-2025
3,270.00
共减 745.00 (23%)
Each
添加至购物车
数量:
6柱
产品类型:
色谱柱
纯化目标:
Top14 Abundant Protein
价格(CNY)
2,525.00
飞享价
Ends: 31-Dec-2025
3,270.00
共减 745.00 (23%)
Each
添加至购物车
Thermo Scientific High Select HSA/免疫球蛋白去除树脂可减少人血浆样品中的高丰度白蛋白和抗体组分,有助于为质谱分析或 1D 或 2D 电泳制备样品。树脂以方便的一次性微型离心柱形式提供,可用于处理 10 µL 样品。

HSA/IgG 去除树脂的特点包括:
•灵活 — 针对处理 10 µL 人血浆样品而调整和优化的微型柱形式
•优化 — 可去除 >95% 的 IgG 和 >95% 的白蛋白
•快速 — 在约10分钟内处理样品
•经济 — 极具性价比的离心柱针对单次使用定价
•减少差异性 — 一次性使用可防止蛋白残留和实验差异

High Select HSA/免疫球蛋白去除树脂使用高特异性固定化抗 HSA 和抗免疫异球蛋白抗体(IgG、IgA、IgM、IgD 和 IgE)去除血清和血浆中的人血清白蛋白 (HSA) 以及所有主要亚类免疫球蛋白。该树脂以经济方便的离心柱形式提供,专门设计用于一步处理和单次使用。

存在高浓度白蛋白和 IgG(可占总血清蛋白的 70% 以上)时,人体液分析通常会变得复杂。去除这些蛋白质通常对于低丰度蛋白的研究来说至关重要。High Select HSA/免疫球蛋白去除树脂能去除超过 95% 的 HSA 和 95% 的 IgG。将样品上样至预填充的一次性离心柱中,在大约10分钟内进行处理。去除高丰度蛋白有助于检测样品中的低丰度蛋白并通过质谱或 1D 或 2D 凝胶电泳进行后续鉴定。

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For Research Use Only. Not for use in diagnostic procedures.
规格
最终产品类型蛋白质
适用于(设备)质谱仪
标签或染料未标记
纯化目标Top14 Abundant Protein
数量6柱
运输条件湿冰
工作流程步骤蛋白去除
产品规格微型离心柱
产品线High Select™
产品类型色谱柱
原始材料血浆,血清, Serum
Unit SizeEach
内容与储存
储存在 2–8°C 下。

常见问题解答 (FAQ)

Can I use the High Select Top14 Abundant Protein Depletion Midi Spin Columns to deplete proteins from rodent samples?

The High Select Top14 Abundant Protein Depletion Midi Spin Columns have only been validated for human samples. We do not recommend using them for protein depletion in rodent samples. For protein depletion in rodent samples, we recommend the following multi-species depletion resins:

  • CaptureSelect MultiSpecies Albumin Depletion Product (Cat. No. 191085305)
  • Pierce Albumin Serum Depletion Kits (Cat. No. 89875)


  • Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are your High Select Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) compatible with cell culture media?

Our High Select Top14 Abundant Protein Depletion Mini Spin Columns have been tested and validated for protein depletion only on human plasma and serum, with each column optimized to bind up to 10 µL of sample.
We have not tested our product on cell culture media or with serum from different species, therefore we do not recommend using this product with cell culture media.

Is there a 96-well format available for the Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369)?

No. The Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) do not come in a 96-well format. However, we do have a Top14 Abundant Protein Depletion Resin (Cat. No. A36372) that can be purchased as a stand-alone product.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After depletion of abundant proteins from my sample, I still see albumin and other abundant proteins after LC-MS analysis. Did the depletion work?

Depletion does not remove all abundant proteins from the sample. Even with 99% depletion of a specific plasma protein, highly abundant proteins will still be the most abundant proteins in the sample detected by MS analysis. However, depletion significantly decreases the most abundant plasma proteins enabling identification of lower abundant proteins.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I proceed to downstream mass spec sample preparation after abundant plasma protein depletion?

After the depletion, samples are significantly diluted in buffer. Depleted samples should be concentrated by speed vac drying, solvent precipitation, or diafiltration. Samples dried using a speedvac are directly compatible with the EasyPep kit chemistry after resuspension using the Lysis Solution.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.