Can I use the High Select Top14 Abundant Protein Depletion Midi Spin Columns to deplete proteins from rodent samples?
The High Select Top14 Abundant Protein Depletion Midi Spin Columns have only been validated for human samples. We do not recommend using them for protein depletion in rodent samples. For protein depletion in rodent samples, we recommend the following multi-species depletion resins:
- CaptureSelect MultiSpecies Albumin Depletion Product (Cat. No. 191085305)
- Pierce Albumin Serum Depletion Kits (Cat. No. 89875)
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Are your High Select Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) compatible with cell culture media?
Our High Select Top14 Abundant Protein Depletion Mini Spin Columns have been tested and validated for protein depletion only on human plasma and serum, with each column optimized to bind up to 10 µL of sample.
We have not tested our product on cell culture media or with serum from different species, therefore we do not recommend using this product with cell culture media.
Is there a 96-well format available for the Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369)?
No. The Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) do not come in a 96-well format. However, we do have a Top14 Abundant Protein Depletion Resin (Cat. No. A36372) that can be purchased as a stand-alone product.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
After depletion of abundant proteins from my sample, I still see albumin and other abundant proteins after LC-MS analysis. Did the depletion work?
Depletion does not remove all abundant proteins from the sample. Even with 99% depletion of a specific plasma protein, highly abundant proteins will still be the most abundant proteins in the sample detected by MS analysis. However, depletion significantly decreases the most abundant plasma proteins enabling identification of lower abundant proteins.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
How do I proceed to downstream mass spec sample preparation after abundant plasma protein depletion?
After the depletion, samples are significantly diluted in buffer. Depleted samples should be concentrated by speed vac drying, solvent precipitation, or diafiltration. Samples dried using a speedvac are directly compatible with the EasyPep kit chemistry after resuspension using the Lysis Solution.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.