TrueCut™ Cas9 Protein v2

The molecular weight of TrueCut Cas9 Protein v2 is ~163 kD

Both products are the same CRISPR-Cas9 protein, but the TrueCut Cas9 Protein is manufactured with a new production process and formulated to meet the standards for Ancillary Materials for Cell and Tissue-Based Products.

Although we do not have a specific antibody recommendation to test the delivery of TrueCut Cas9 Protein v2, we have tested the following antibodies against an older version of Cas9 (Platinum Cas9) using U2OS-Cas9 expression cell lines (generated using Cas9 lentivirus):
- Cas9 Recombinant Polyclonal Antibody (Cat. No. 711061)
- Cas9 Monoclonal Antibody (Cat.No. MA1-201)
- Cas9 Monoclonal Antibody (Cat. No. MA1-202)

Our TrueCut Cas9 Protein v2 and the Platinum Cas9 have similar domains, and the TrueCut Cas9 Protein v2 has better efficiencies. Therefore, these antibodies should work with TrueCut Cas9 Protein v2 as well.

The storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein is as follows:
15 mM Tris-HCl pH 8.0, 150 mM NaCl, 1.0 mM TCEP, 50% glycerol.

We recommend using Lipofectamine CRISPRMAX Transfection Reagent with TrueCut Cas9 Protein v2 or TrueCut HiFi Cas9 Protein.