Ion AmpliSeq ERCC Companion Panel - FAQs

View additional product information for Ion AmpliSeq ERCC Companion Panel - FAQs (A36552)

7 product FAQs found

How many reactions can I perform with the Ion AmpliSeq RNA ERCC Companion Panel?

The Ion AmpliSeq RNA ERCC Companion Panel provides enough reagents to perform 96 manual reactions or 48 automated reactions.

What software do I need to view data from the Ion AmpliSeq RNA ERCC Companion Panel?

To view data from the Ion AmpliSeq RNA ERCC Companion Panel, Torrent Suite Software version 5.10 or later is required and Version 5.10.0.3 or later of the ERCC_Analysis plugin for Torrent Suite Software is required.

Can the Ion AmpliSeq RNA ERCC Companion Panel be used with FFPE samples?

We do not recommend using the Ion AmpliSeq RNA ERCC Companion Panel with FFPE samples or with any panels besides human and mouse Ion AmpliSeq transcriptome gene expression panels.

How much of the Ion Ampliseq RNA ERCC Companion Panel should I add to the target amplification reactions?

We recommend adding 1 µL of the Ion Ampliseq RNA ERCC Companion Panel per manual target amplification reaction and 8 µL per primer pool for an automated target amplification reaction on the Ion Chef Instrument.

Why would one use the Ion AmpliSeq ERCC Companion Panel?

The Ion AmpliSeq ERCC Companion Panel is compatible with Ion AmpliSeq Transcriptome Human Gene Expression Kit and Ion AmpliSeq Transcriptome Mouse Gene Expression Kit (not recommended for use with FFPE samples or panels outside of Ion AmpliSeq Human or Mouse Transcriptome kits). With the addition of the Ion AmpliSeq RNA ERCC Companion panel to your Ion AmpliSeq RNA panel, dynamic range and sensitivity can be similarly evaluated in Ion AmpliSeq RNA libraries that are prepared from RNA samples containing the ERCC RNA Spike-In Mix

How many primer pairs are in the Ion AmpliSeq RNA ERCC Companion Panel?

The Ion AmpliSeq RNA ERCC Companion Panel consists of 10 primer pairs targeting 10 transcripts from the ERCC RNA Spike-In Mix representing a 13log2 dynamic range. Chosen for general GC balance and amenability for linear amplification (TaqMan qPCR), the selected ERCC targets produce R2 > 0.9 under normal experimental conditions.

Which kits are compatible with the Ion AmpliSeq RNA ERCC Companion Panel?

The Ion AmpliSeq RNA ERCC Companion Panel is compatible with both manual and chef-ready versions of human and mouse Ion AmpliSeq transcriptome gene expression kits:

- Ion AmpliSeq Transcriptome Human Gene Expression Kit (Cat. No. A26325, A26326, or A26327)
- Ion AmpliSeq Transcriptome Mouse Gene Expression Kit (Cat. No. A36553, A36554, or A36555)
- Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit (Cat. No. A31446)
- Ion AmpliSeq Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit (Cat. No. A36412)