1 mL 沉淀微珠。4 mL,以 25% 悬浮液(溶于含 0.02% 叠氮化钠的 PBS)形式提供。
在 2–8°C 下储存。
常见问题解答 (FAQ)
How does Pierce Anti-DYKDDDDK Magnetic Agarose (Cat. No. A36797, A36798) work for purification of DYKDDDK-tagged recombinant proteins?
The amino acid sequence DYKDDDDK, commonly known as the ''Flag'' epitope group, is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle.
For protein purification, the magnetic agarose is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or the C-terminus. Captured proteins are then magnetically separated from the supernatant, and non-specifically bound proteins can be washed away before dissociating bound DYKDDDDK-tagged proteins with elution buffer. The magnetic agarose is removed from the solution using a magnetic stand or an instrument such as the KingFisher Flex Magnetic Particle Processor. Automated instruments are especially useful for higher-throughput purifications and screening of purification conditions.
What is the slurry/suspension percentage for your anti-DYKDDDDK ("FLAG") products?
Anti-DYKDDDDK Magnetic Agarose (Cat. Nos. A36797, A36798, A36799B) is offered as a 25% suspension (1 mL of 25% suspension = 0.25 mL of settled beads). UltraLink-based Anti-DYKDDDDK Affinity Resin (Cat. Nos, A36801, A36803, A36804) is offered as a 50% slurry (1 mL of 50% slurry = 0.5 mL of settled resin).
How do I cleave off the DYKDDDDK ("FLAG") tag after purification?
An enterokinase cleavage site behind the DYKDDDDK (FLAG) tag can allow complete removal of the DYKDDDDK (FLAG) tag leaving no additional amino acids. We offer EKMax Enterokinase (Cat. Nos. E18001 and E18002) that can be used for this purpose. Subsequently, the EKMax Enterokinase can be removed using EK-Away Resin (Cat. No. R18001), a resin conjugated with soybean trypsin inhibitor, which has high affinity for enterokinase.