CTS™ OpTmizer™ T-Cell Expansion SFM, no phenol red, bottle format - FAQs

View additional product information for CTS™ OpTmizer™ T-Cell Expansion SFM, no phenol red, bottle format - FAQs (A3705001)

6 product FAQs found

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer the CTS OpTmizer Expansion Basal Medium and CTS OpTmizer Expansion Supplement (included in the CTS OpTmizer T-Cell Expansion SFM (Cat. No. A3705001)) as stand-alone products?

No, the CTS OpTmizer Expansion Basal Medium and CTS OpTmizer Expansion Supplement are not sold separately.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there a difference in formulation of the CTS OpTmizer T Cell Expansion SFM between the bottle and bag packaging formats?

The bottle and bag packaging formats of the medium have the same formulation.

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Can I use CTS OpTmizer T Cell Expansion SFM for culture and expansion of mouse T cells? If not, which medium would you recommend?

We have not tested this medium on mouse T cells. If you're looking for a serum-free medium to culture mouse T cells, you can try AIM V Medium (Cat. No. 12055083 or 12055091). For mouse cells, we recommend adding 50 µM beta-mercaptoethanol (Cat. No. 21985023).

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Can GlutaMAX be used instead of Glutamine to supplement CTS OpTmizer T-Cell Expansion SFM?

GlutaMAX cannot replace Glutamine because cells may not initially use GlutaMAX so that T cells cannot grow. We recommend adding 4 mM GlutaMAX and 2 mM Glutamine (as stated in the manual) into the CTS OpTmizer T-Cell Expansion SFM if GlutaMAX is needed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of CTS OpTmizer T-Cell Expansion SFM?

The complete CTS OpTmizer T-Cell Expansion SFM is stable for 4 weeks when stored in the dark at 2-8 degrees C. However, the two individual medium components are stable for 18 months when stored in the dark at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.