Chemical visualization of an attractant peptide, LURE.
AuthorsGoto H, Okuda S, Mizukami A, Mori H, Sasaki N, Kurihara D, Higashiyama T,
JournalPlant Cell Physiol
PubMed ID21149297
The pollen tube attractant peptide LUREs of Torenia fournieri are diffusible peptides that attract pollen tubes in vitro. Here, we report a method enabling the direct visualization of a LURE peptide without inhibiting its attraction activity by conjugating it with the Alexa Fluor 488 fluorescent dye. After purifying and refolding ... More
Bacterial particle endocytosis by epithelial cells is selective and enhanced by tumor necrosis factor receptor ligands.
AuthorsClark RT, Hope A, Lopez-Fraga M, Schiller N, Lo DD,
JournalClin Vaccine Immunol
PubMed ID19129472
Bacterial pathogens use virulence strategies to invade epithelial barriers, but active processes of epithelial cells may also contribute to the endocytosis of microbial particles. To focus on the latter, we studied the uptake of fixed and fluorescently labeled bacterial particles in intestinal and bronchoepithelial cell cultures and found it to ... More