• 1 Oncomine 肿瘤突变负荷检测,可供 32 次反应,储存于 -5 至 -30°C。 • 4 卡盒 Ion AmpliSeq Chef Reagents DL8,储存于 -5 至 -30°C。 • 4 卡盒 Ion AmpliSeq Chef Solutions DL8,储存于 2–8°C。 • 4 盒 Ion AmpliSeq 耗材,室温储存。 •1 IonCode in PCR 孔板,室温储存。
常见问题解答 (FAQ)
With the Oncomine Tumor Mutation Load Assay, do I need to adjust the PCR cycles based on sample type?
No, the Oncomine Tumor Mutation Load Assay is optimized for use with FFPE tissue. The in-house development of the assay specifically focused on three tissue types: melanoma, lung, and colon.
In the Oncomine Tumor Mutation Load Assay, can I get the total variant calls (i.e., not just somatic)?
Yes, total variant calls are quantified and reported by the Oncomine Tumor Mutation Load Assay workflow and a .vcf file of all called variants is available to download in the user interface.
In the Oncomine Tumor Mutation Load Assay, does the germline filter cover across different ancestry types?
Yes, germline filters cover across different ancestry types. They are not ethnicity-based, but are global population-based.
Can the Oncomine Tumor Mutation Load Assay (TML) workflow 2.0 be edited to run on other panels (such as Oncomine Comprehensive Assay v3)?
Yes, by following the instructions in the appendix of the User guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0015885_OncomineComprehensiveAssay_v3_UG.pdf).
Can I run the Oncomine Comprehensive Assay BAM file through the Ion Reporter workflow for the Oncomine Tumor Mutation Load (TML) Assay?
Yes, Oncomine Comprehensive Assay BAM files can be used with the Oncomine TML workflow. Targets that are not in Oncomine TML workflow will just be discarded during the analysis.